General information for g10020.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g10020 is located on chr_1: 6753264..6756214 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10020 | g10020.t1 | TSS | g10020.t1 | 6753252 | 6753252 |
| chr_1 | g10020 | g10020.t1 | isoform | g10020.t1 | 6753264 | 6756214 |
| chr_1 | g10020 | g10020.t1 | exon | g10020.t1.exon1 | 6753264 | 6755800 |
| chr_1 | g10020 | g10020.t1 | cds | g10020.t1.CDS1 | 6753264 | 6755800 |
| chr_1 | g10020 | g10020.t1 | exon | g10020.t1.exon2 | 6755923 | 6756214 |
| chr_1 | g10020 | g10020.t1 | cds | g10020.t1.CDS2 | 6755923 | 6756214 |
| chr_1 | g10020 | g10020.t1 | TTS | g10020.t1 | 6756248 | 6756248 |
Sequences
>g10020.t1 Gene=g10020 Length=2829
ATGTGGAAATTTTTAATAAAAATTCTCTTTATAGGACTGTTTGTAAGTTTCAGTACTTCT
GATGACTTTCAAAATTTTAAAGAAACAAAAATTCGTAAAACAGAAAGTAGTGCAATTCAA
TCAAGTGAAACTTTAACATATCGATTACCAAATAATTCTTTTCCACTTGCTTATGATATC
ATACTTAAAACAGACATCGACAAGGGTGAATTTGCTTTTACAGGCAAAGTGAAAATTCAT
GTCAAAGTTATTGAGGCAACAGATAAAATAACGTTACATTATAAACAAATAGAAATCGAT
ACAATAAATCTGTTACAAAATGATAGTCTAACTTTAATTTCTTCAAACCTGCAATTTTCG
CTAATTCAATCACATAGTTTTCTGATTATTTCATTACCATTCGTTTATCAATCTGATAGT
GAATTTGTGCTTGAAGTTTCATATCATGGTGAGTTACGCGACGATGCCGCTGGTTTTTAT
CGCGCAAACTACACTACAGGTGATGGAAATGTCATTTGGTTTGCCACAACACAGTTTGAG
ATTCACGATGCACGTCATGCTATGCCGTGTTATGATGAGCCTGCAATTCGAGCTCCAATG
GCATTGACAATTGTGCATGGAAAAAATTATTCGGCTGTTGCCAACATGGAAGTTGATGAA
GTACATGAAATTGATGCTGAATATGTTGAAACAAAATTTAAACCAACGCCATCAATGCAA
ACTTATTTATTGGCATTTTTAGTAAGTGATTTTGAATCAATTGATGCAATTGACTCAAGA
ATTCCTCAGAAAATTTATGCCAAACCTATGTCAATTAGAAACAATGAAGCTAACTTTTCA
GCTAATGTCGTTGGACCAATTTTGAGAGCACATGAGAATCATTTAGGTATCAATTATCCA
CTCAATAAAATGGACCATGCTGCAATAACACAATTCACATTCGGTGCTATGGAAAATTTT
GGTTTAATCACATATTTGGAGCGTGCTTTGCTTTTAAATCCAAACACATCAGCAGATTTC
GCTGAAGCTTCAAAATTTCTAATAATTCGCATCATTGCACATGAATATGCACACCAATAT
TTTGGTAATATTGTGTCACCACAATGGTGGTCTTACACATTTTTAAATGAAGGACTTGCT
ACGCTTTTTGAAGTTCTTATTCCGAATATGCTCTATCCTGAAATGAAGTTTATGGATCGC
TTTGTTACTCATAATATGCCTCAAGCATTTCTCGCTGACAGTATTAATGCTTGGTCAATG
AGTCACTATACTGAACATCCTGATGAATTATGGACAAAATTCAATAACATTGGCTATGAA
AAATCAGGTTGCATAATGAGAATGTTTCAAGAAGTTTTGACACCACAAACTTTTGCTAAA
GGATTAAATTTATATTTGAATGACATGTATTTTAAAGCAGCAACACCTGATGATTTGCAT
AGAAATTTGCAAGCTGTTTATGATGTTGACTTTCCATCAAATTCATTGGATATTGGTGAA
GCAATGCTTACATGGGAAAATCAAGCTGGCTATCCATTGATAACAGTGCAAATTTATGAC
AACCATTTCATTCTTACTCAACGTCGCTATCCTGTTAGCAACGGTGAAATCTATGCAGTT
CCAATAACAATAGCAACCAAAACTCGTCATGATTTTAATAAAAAGACACCGAAAGTTTGG
TTGACAACTGAAAATGCTGCATTTTCACACTCTGAATTTGAATTTAATGTTACAAACGAT
GACTGGATTATTTTGAATATTCAGCAAATCGGATATTATCGTATTGATTATGACATAAAT
CTATGGCATTCAATCATTGCACAGCTTATAGAAAGTCCTGAAACAATCAATTCAATAAAT
CGTGCTGTTTTGCAAGATGAAATTTATTTAGCATGGAGTGAATTAAATCGAGTAAAAGCA
GATGATGTTTTGAACATCTTAAGCTACTTTGACAGGGAAAACGAACCAATAGCTTGGAGC
AAAGCACAAACAACTATTGCAACACTCAATAATCGTCTCTTTGGTACTGAAATTTATGAA
AAGTTTTTGAATTATCTCATAAATATCACAACATCGCATGTCAAAGAGCTTGGCTATGAT
CGAATCACTGATGAGTCATCAACCATCAGTTCCTTAAGAAGTTCAACAAAATCATGGAAT
TGTATTGCATTAGATGAAAATTGCTTAAATCATGAACTGAAGAAGTTGCGAGATTATCTC
ACTTTACCGAGTGCAAGTGCAGCTTTCGACTATTGTCATGGTCTACGACTCATTGATGTA
AAAACTTTTTACAATTTAACACAAGCTGTACAATTTAATGAATCATTTGAAAATCGTGAA
AATTTTCTTAACAATCTTGGATGCACATTGAATGAAGAAAATTTAAAACTTTTGTTAAAT
ATTTCAATCTCAGTGAATAATAAACTGAGTTTGGCTGATAGAAGTAACTTGTTGATAAAC
ACAATGGGAAGAAGTGTAGTTGCATTGAATTTGACATTAGATTTTCTAATTTCTAATTTC
AATTTGGTCAACATGCGAATCCCATCATCTTTCAAAACTATTTTATCTTCAATGGCTTCC
TACATAAATGTACAACCGCATATTGAAAAAACTGAAGAACTACTAACAATTCTTTTGAGA
GATACAATTATCGATAAAAATGACGCAATTAATGTCAGAAATATTTACCTTAAGAATATC
AAATGGCAAATTGACAACTATGATGATATTGAAAAATGGTTTGCAATAAATAGCGGGAAG
AAATTGATGATTTCTATTGGAATTATTGTGATTTGTAATTTTGTAAGCTTTTTGAGACGA
TTTTTGTAA
>g10020.t1 Gene=g10020 Length=942
MWKFLIKILFIGLFVSFSTSDDFQNFKETKIRKTESSAIQSSETLTYRLPNNSFPLAYDI
ILKTDIDKGEFAFTGKVKIHVKVIEATDKITLHYKQIEIDTINLLQNDSLTLISSNLQFS
LIQSHSFLIISLPFVYQSDSEFVLEVSYHGELRDDAAGFYRANYTTGDGNVIWFATTQFE
IHDARHAMPCYDEPAIRAPMALTIVHGKNYSAVANMEVDEVHEIDAEYVETKFKPTPSMQ
TYLLAFLVSDFESIDAIDSRIPQKIYAKPMSIRNNEANFSANVVGPILRAHENHLGINYP
LNKMDHAAITQFTFGAMENFGLITYLERALLLNPNTSADFAEASKFLIIRIIAHEYAHQY
FGNIVSPQWWSYTFLNEGLATLFEVLIPNMLYPEMKFMDRFVTHNMPQAFLADSINAWSM
SHYTEHPDELWTKFNNIGYEKSGCIMRMFQEVLTPQTFAKGLNLYLNDMYFKAATPDDLH
RNLQAVYDVDFPSNSLDIGEAMLTWENQAGYPLITVQIYDNHFILTQRRYPVSNGEIYAV
PITIATKTRHDFNKKTPKVWLTTENAAFSHSEFEFNVTNDDWIILNIQQIGYYRIDYDIN
LWHSIIAQLIESPETINSINRAVLQDEIYLAWSELNRVKADDVLNILSYFDRENEPIAWS
KAQTTIATLNNRLFGTEIYEKFLNYLINITTSHVKELGYDRITDESSTISSLRSSTKSWN
CIALDENCLNHELKKLRDYLTLPSASAAFDYCHGLRLIDVKTFYNLTQAVQFNESFENRE
NFLNNLGCTLNEENLKLLLNISISVNNKLSLADRSNLLINTMGRSVVALNLTLDFLISNF
NLVNMRIPSSFKTILSSMASYINVQPHIEKTEELLTILLRDTIIDKNDAINVRNIYLKNI
KWQIDNYDDIEKWFAINSGKKLMISIGIIVICNFVSFLRRFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 25 | g10020.t1 | CDD | cd09601 | M1_APN-Q_like | 58 | 505 | 1.93579E-152 |
| 15 | g10020.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 36 | 251 | 1.8E-59 |
| 16 | g10020.t1 | Gene3D | G3DSA:1.10.390.60 | - | 255 | 510 | 3.3E-68 |
| 14 | g10020.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 511 | 597 | 9.9E-17 |
| 17 | g10020.t1 | Gene3D | G3DSA:1.25.50.20 | - | 598 | 916 | 1.8E-37 |
| 4 | g10020.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 34 | 908 | 4.0E-193 |
| 5 | g10020.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 34 | 908 | 4.0E-193 |
| 6 | g10020.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 185 | 200 | 3.0E-21 |
| 8 | g10020.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 233 | 248 | 3.0E-21 |
| 9 | g10020.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 312 | 322 | 3.0E-21 |
| 10 | g10020.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 351 | 366 | 3.0E-21 |
| 7 | g10020.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 370 | 382 | 3.0E-21 |
| 3 | g10020.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 55 | 243 | 3.6E-43 |
| 2 | g10020.t1 | Pfam | PF01433 | Peptidase family M1 domain | 279 | 505 | 1.4E-53 |
| 1 | g10020.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 582 | 875 | 2.2E-31 |
| 20 | g10020.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 21 | g10020.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 22 | g10020.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 24 | g10020.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 19 | g10020.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 921 | - |
| 23 | g10020.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 922 | 941 | - |
| 18 | g10020.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 942 | 942 | - |
| 12 | g10020.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 44 | 250 | 1.96E-47 |
| 11 | g10020.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 260 | 516 | 2.2E-62 |
| 13 | g10020.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 26 | g10020.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 27 | g10020.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 922 | 939 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K01256 (aminopeptidase N)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed