General information for g10195.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamate–cysteine ligase.
Parent gene
- The parent gene g10195 is located on chr_1: 8167819..8169830 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10195 | g10195.t5 | isoform | g10195.t5 | 8166261 | 8169830 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon1 | 8166261 | 8166832 |
| chr_1 | g10195 | g10195.t5 | TTS | g10195.t5 | 8166281 | 8166281 |
| chr_1 | g10195 | g10195.t5 | cds | g10195.t5.CDS1 | 8166649 | 8166832 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon2 | 8166901 | 8167752 |
| chr_1 | g10195 | g10195.t5 | cds | g10195.t5.CDS2 | 8166901 | 8167752 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon3 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t5 | cds | g10195.t5.CDS3 | 8167819 | 8168250 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon4 | 8168308 | 8168614 |
| chr_1 | g10195 | g10195.t5 | cds | g10195.t5.CDS4 | 8168308 | 8168614 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon5 | 8168688 | 8168846 |
| chr_1 | g10195 | g10195.t5 | cds | g10195.t5.CDS5 | 8168688 | 8168817 |
| chr_1 | g10195 | g10195.t5 | exon | g10195.t5.exon6 | 8169597 | 8169830 |
| chr_1 | g10195 | g10195.t5 | TSS | g10195.t5 | NA | NA |
Sequences
>g10195.t5 Gene=g10195 Length=2556
TTATTCTATTTTTAATATATTTTTTTGCAGAAAAAAATCTTTTTATTATTTTTAAGAAAA
ATTTAGTAGGCGATCACACTGCATTGCACATCCCCCTCAGTAAACTTGATACCTCTCGTA
TTTAGACAGAAATTTCACAATACATAGTTTATATTTTGTTTGAAAATTTAATCATTATTT
GCAGATTTAGACTTTTAATCAAAACAATTGAAATAATTCGTGAATTGTCAAAAGAATATC
ACTACGTTTTAAATTAATTTGTAATGGGATTATTGACAGAAGGCAGTCCACTTTCATGGG
AGGAGACAAAAAATTTGGCACAGCATGTGAGAAAACATGGTATCAAGCAGTTTATTAACT
TATTTGCAAGATTAAAAGACCGTCAAGGTGATGTACTAAAATGGGGTGATGAAGTTGAGT
ATATGATTATAAGGTTTGATGACGATAAACAAATAGCACAAGCCTCTTTAAGAGCACTTG
AAATACTGAACGTGTTACAAGAACCGGAACTGCGCGATCCAGATGGAGTAAAGTCACTTT
GGCGCCCTGAATATGCGGCATATATGGTCGAAGGTACACCTGGTAAACCCTATGGAGGGC
TATTAGCTCACTTTAATGTTGTTGAATCATCAATGAAATACCGTAGAGAAGAAGTGTCCA
AGTTGTTAAAAGATGGTGAAGAAGTTATGTCGATTACAAGTTTTCCACGTTTGGGTTGTC
CACAATTTTCATATCCATCATATGATGTGCAACCAGACAATCCACAATCTGCAGCTCAAT
CATTATTCTTTCCTGATGAAGCTATATATTCGGGTCATCCACGTTTCAAAAATTTAACGC
GAAATATTAGATTAAGACGAGGTGAAAAGGTGTGCATCAACGTTCCAGTTTTTAAAGATG
AAAACACAAAGTATCCTATTCCTTATTCACTGCCTGAAACGCCTGATCATGTTTATATGG
ATGCGATGGGTTTCGGTATGGGAAATTGTTGCTTGCAATTAACATTTCAAGCGTGTAATA
TCAATGAAGCTAGAGTTCTTTATGATCAATTGACTCCTCTCTGTCCAATTATGTTAGCAT
TGACTGCTGCATCTCCCTTATATAGGGGCTTTTTAACTGATATCGATTGCAGATGGAATG
TTATTTCAGCATCTGTAGATTGTAGAACAAAAGAGGAAAGAGGTCTTGAACCTCTAAAAG
AAAATAAATTTAGAATCAACAAGTCACGCTATGATTCAATCGATTCTTATCTATCTGAGG
AGGGCGAAAAATACAACGACGTTCCTTTACTTTACAATGAAGAAGACTATAAAACTTTAC
GTGAAGGTGGAATTGATCATTTATTGGCACAGCATATTGCCCATTTATTTATTCGGGATA
CTGTTTCTTTATTCAGTGAAAAAATTCATCAAAATGATGAAGAAGAGACCGATCATTTCG
AAAACATTCAATCAACAAATTGGCAAACAATGCGATTTAAACCACCACCACCTTCGAGTA
GCATTGGCTGGCGAGTCGAATTCCGTCCTTGTGAACTCCAATTAACGGATTTTGAGAATG
CTGCAATTGTTGTTTTCATTGTATTGTTAACGCGTGTTATACTCTCATTCAAACTTAATT
TCCTTATACCAATCAGTAAAGTTGATGAGAATATGCAAAAGGCACAAAAACGAAATGCTT
GTAAAGAAGAAAAATTTTGGTTCCGAAGAAATTTCAACGGTAAAAAAGAAGACAATAATT
CCTCTGAAAATAATGAATGTCAAGATTGTGAGCTTTGGACTATTAATGAAATCATTAATG
GAAAAGAAAGTTTCCCTGGACTAATTAGATTAATAAATCAATATTTAGCTTCAATGGAGG
TCGACACTGACACACACTGCTCAATTCAGCAATATTTGAAATTAATACAGCGCAGAGCTG
CCGGAAAATTGTTAACAACTGCCTCTTGGATACGTAAAGAAGTATTAAATCATCCAGATT
ATAAGAAGGATTCTATTGTAAGCGAAAAAATCAACTATGATTTGTTAAAGAAAGCAAAGA
AAATTCAAGAAGGTCTCTTACCGTGTAATGAGCTTATAGGATGTTTCACAAATTCCAAAA
CGAAAGAAGAAATTCCCCCCGCCATTGCGAAACAACATCAACCAAAAATGCCTAATGGAA
CATCGTAATGGATTTGTAATGATAATTGAATGTCGCCTATGAATACTAGAAAAATAGAAA
GTGCACTTAATTATTAATTACATTTGATGATAGTGAACATGGCGATTAAATTTTTAAGAA
AGAGAGAGATAATATAAATGTATGTTAATGTTTCATATAGTAATGATATATGCATATTAT
ATATTTTAATAGAACTAACTACCTTCAAATATTAACTTTTTAAATTATGCTTATTGCTTA
TACAGTATAAATTTTTTTAGTATATAGTAGTACAAAAGACACATTCTTACATACTTATAT
GTTTCAATATACTTTTTCTTATAAAACTGTAACTTGATAAAATTTATAATAAAGTAGTTC
AAAGTTGGTATCATATAGAAACAGCATTATTTTATG
>g10195.t5 Gene=g10195 Length=634
MGLLTEGSPLSWEETKNLAQHVRKHGIKQFINLFARLKDRQGDVLKWGDEVEYMIIRFDD
DKQIAQASLRALEILNVLQEPELRDPDGVKSLWRPEYAAYMVEGTPGKPYGGLLAHFNVV
ESSMKYRREEVSKLLKDGEEVMSITSFPRLGCPQFSYPSYDVQPDNPQSAAQSLFFPDEA
IYSGHPRFKNLTRNIRLRRGEKVCINVPVFKDENTKYPIPYSLPETPDHVYMDAMGFGMG
NCCLQLTFQACNINEARVLYDQLTPLCPIMLALTAASPLYRGFLTDIDCRWNVISASVDC
RTKEERGLEPLKENKFRINKSRYDSIDSYLSEEGEKYNDVPLLYNEEDYKTLREGGIDHL
LAQHIAHLFIRDTVSLFSEKIHQNDEEETDHFENIQSTNWQTMRFKPPPPSSSIGWRVEF
RPCELQLTDFENAAIVVFIVLLTRVILSFKLNFLIPISKVDENMQKAQKRNACKEEKFWF
RRNFNGKKEDNNSSENNECQDCELWTINEIINGKESFPGLIRLINQYLASMEVDTDTHCS
IQQYLKLIQRRAAGKLLTTASWIRKEVLNHPDYKKDSIVSEKINYDLLKKAKKIQEGLLP
CNELIGCFTNSKTKEEIPPAIAKQHQPKMPNGTS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g10195.t5 | Coils | Coil | Coil | 457 | 477 | - |
| 5 | g10195.t5 | Gene3D | G3DSA:3.30.590.50 | - | 42 | 322 | 1.3E-108 |
| 6 | g10195.t5 | Gene3D | G3DSA:3.30.590.50 | - | 381 | 470 | 1.1E-32 |
| 4 | g10195.t5 | Gene3D | G3DSA:1.10.8.960 | - | 557 | 613 | 4.5E-18 |
| 2 | g10195.t5 | PANTHER | PTHR11164 | GLUTAMATE CYSTEINE LIGASE | 1 | 612 | 1.2E-279 |
| 1 | g10195.t5 | Pfam | PF03074 | Glutamate-cysteine ligase | 230 | 594 | 1.2E-179 |
| 9 | g10195.t5 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 432 | - |
| 10 | g10195.t5 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 433 | 455 | - |
| 8 | g10195.t5 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 456 | 634 | - |
| 3 | g10195.t5 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 39 | 583 | 1.96E-58 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004357 | glutamate-cysteine ligase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K11204 (glutamate–cysteine ligase catalytic subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
