General information for g10216.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Thiamine transporter 1.
Parent gene
- The parent gene g10216 is located on chr_1: 8269859..8271651 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10216 | g10216.t1 | TSS | g10216.t1 | 8268233 | 8268233 |
| chr_1 | g10216 | g10216.t1 | isoform | g10216.t1 | 8268356 | 8269859 |
| chr_1 | g10216 | g10216.t1 | exon | g10216.t1.exon1 | 8268356 | 8268567 |
| chr_1 | g10216 | g10216.t1 | cds | g10216.t1.CDS1 | 8268356 | 8268567 |
| chr_1 | g10216 | g10216.t1 | exon | g10216.t1.exon2 | 8268630 | 8268770 |
| chr_1 | g10216 | g10216.t1 | cds | g10216.t1.CDS2 | 8268630 | 8268770 |
| chr_1 | g10216 | g10216.t1 | exon | g10216.t1.exon3 | 8268825 | 8268985 |
| chr_1 | g10216 | g10216.t1 | cds | g10216.t1.CDS3 | 8268825 | 8268985 |
| chr_1 | g10216 | g10216.t1 | exon | g10216.t1.exon4 | 8269051 | 8269859 |
| chr_1 | g10216 | g10216.t1 | cds | g10216.t1.CDS4 | 8269051 | 8269859 |
| chr_1 | g10216 | g10216.t1 | TTS | g10216.t1 | 8269917 | 8269917 |
Sequences
>g10216.t1 Gene=g10216 Length=1323
ATGCAGCAGTGGCTAAAAATTTCTCTAGCTCTTTGTTCTTTTGGATTCTTCAGAGAGTTT
AGACCTTCAGAGCCATTTGTTACTAATTTTTTAACATCAACTAAATGGAGAAATTTAACT
GAAGATCAAATAAATTTTGAAGTTTATCCAGTCGGCACATATTCGACACTTACACTACTT
GTTTTCGTATTTTTAGTGACTGATATATTAAGATATAAACCAATTATCATCGCTTCATCA
TGTGCAGGTATAATTATTTGGTCGATTCTACTCTGGACTACATCATTACTTGCATTACAA
ATTTCACAATTCTTTTATGGGTTCTATATGGCAGCTGAAGTTGCATATTATACTTACATG
TATGCTAAAGTAGAAAAAAGTAAATATCAAAAAGTTACGGGAAACGCTCGCGCTGCTATA
TTGACTGGACGTTTTACATCAAGTGTAATTGCTCAACTTTTATATTCATTCGATATTATG
GATTTTAGAGAATTAAATTATTTAACACTTGGAGCTCAATCCATATCATTGATTATTGCT
ATCTTTTTACCATCTGTTGGCATAAGCCTATATTTTTATAAAGTTCCTAATGAAGAACAA
AATGATTCAACAATAACTGATAGATCTTCATCAAGTACTAATGTAAAACCAAGATTTTCT
TATAATAGAGCTGGAACTCTTTTATGGAAGCATTTTCTTGAAGCTTACTCGAATCCAATA
GTAATTCAATATAGTTTTTGGTGGGCACTTGCTATGGCAGGATTTTTCATGGTACAATTT
TATGTACAATTTCTTTGGTTACTCATTGAAGATCGAGAAGTTTATAATGCAGGTGTTGAA
GCAGTTTTGACATTATTTGGTGCATTGACTGCTTTTTTTGCTGGTTATTTAACGAGTAGG
ACATTCAAAAAATATGATCTTTGGATTCTGGCAATATGTTCATTCTTGCAAGGAGCAATG
GTACTGGTGTCTTCACAATCAAACAATATTTGGGTCATGTACTCAATGTATGTTTTGTTT
GGCACCTTCTTTAGTTTTATGATAACGATTGCTACTGCTTTTGTTGCTCAACAACTTGCA
GACGATAGTTTTGCATTAATTTTTGGTATCAATACTTTAATTGCATTAATATTCCAAACT
ATTCTCACTTTTGTAATTATGACTTGGCTTAATTTACAGACTCGAATACAATTTTTTATT
TTTGGCTGTTACTTTATAGTATTAGCTGCAATCTACTTGATTACTGCAATAATAAAAGCA
GTATTTTGGGGAAATGATGCGTCGAATACATATCAGATTAATAATGATATAAAGGAAAAT
TAG
>g10216.t1 Gene=g10216 Length=440
MQQWLKISLALCSFGFFREFRPSEPFVTNFLTSTKWRNLTEDQINFEVYPVGTYSTLTLL
VFVFLVTDILRYKPIIIASSCAGIIIWSILLWTTSLLALQISQFFYGFYMAAEVAYYTYM
YAKVEKSKYQKVTGNARAAILTGRFTSSVIAQLLYSFDIMDFRELNYLTLGAQSISLIIA
IFLPSVGISLYFYKVPNEEQNDSTITDRSSSSTNVKPRFSYNRAGTLLWKHFLEAYSNPI
VIQYSFWWALAMAGFFMVQFYVQFLWLLIEDREVYNAGVEAVLTLFGALTAFFAGYLTSR
TFKKYDLWILAICSFLQGAMVLVSSQSNNIWVMYSMYVLFGTFFSFMITIATAFVAQQLA
DDSFALIFGINTLIALIFQTILTFVIMTWLNLQTRIQFFIFGCYFIVLAAIYLITAIIKA
VFWGNDASNTYQINNDIKEN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g10216.t1 | Gene3D | G3DSA:1.20.1250.20 | MFS general substrate transporter like domains | 25 | 418 | 8.5E-21 |
| 2 | g10216.t1 | PANTHER | PTHR10686:SF36 | AGAP006349-PA | 1 | 425 | 1.3E-161 |
| 3 | g10216.t1 | PANTHER | PTHR10686 | FOLATE TRANSPORTER | 1 | 425 | 1.3E-161 |
| 33 | g10216.t1 | PIRSF | PIRSF028739 | Folate/thiamine_transporter | 1 | 439 | 8.8E-137 |
| 1 | g10216.t1 | Pfam | PF01770 | Reduced folate carrier | 2 | 399 | 2.0E-139 |
| 18 | g10216.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 19 | g10216.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 20 | g10216.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 17 | - |
| 32 | g10216.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 13 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 45 | - |
| 27 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 46 | 66 | - |
| 11 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 67 | 74 | - |
| 28 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 75 | 98 | - |
| 12 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 99 | 103 | - |
| 29 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 104 | 122 | - |
| 7 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 123 | 133 | - |
| 22 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 134 | 155 | - |
| 14 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 156 | 174 | - |
| 30 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 175 | 193 | - |
| 10 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 194 | 245 | - |
| 23 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 246 | 269 | - |
| 16 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 270 | 280 | - |
| 25 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 281 | 298 | - |
| 9 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 299 | 304 | - |
| 26 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 305 | 325 | - |
| 15 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 326 | 330 | - |
| 31 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 331 | 356 | - |
| 6 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 357 | 362 | - |
| 21 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 363 | 390 | - |
| 17 | g10216.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 391 | 395 | - |
| 24 | g10216.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 396 | 418 | - |
| 8 | g10216.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 419 | 440 | - |
| 4 | g10216.t1 | SUPERFAMILY | SSF103473 | MFS general substrate transporter | 52 | 415 | 2.88E-23 |
| 45 | g10216.t1 | TIGRFAM | TIGR00806 | rfc: reduced folate carrier | 1 | 423 | 2.0E-99 |
| 40 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 44 | 66 | - |
| 35 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 76 | 98 | - |
| 42 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 105 | 122 | - |
| 43 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 135 | 157 | - |
| 44 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 170 | 192 | - |
| 41 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 246 | 268 | - |
| 34 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 275 | 297 | - |
| 36 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 307 | 325 | - |
| 37 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 332 | 354 | - |
| 39 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 364 | 386 | - |
| 38 | g10216.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 421 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0090482 | vitamin transmembrane transporter activity | MF |
| GO:0051180 | vitamin transport | BP |
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K14610 (solute carrier family 19 (thiamine transporter), member 2/3)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
