General information for g10249.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Octopamine receptor beta-2R.
Parent gene
- The parent gene g10249 is located on chr_1: 8522535..8525843 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10249 | g10249.t1 | TSS | g10249.t1 | 8522444 | 8522444 |
| chr_1 | g10249 | g10249.t1 | isoform | g10249.t1 | 8522535 | 8525843 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon1 | 8522535 | 8522858 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS1 | 8522535 | 8522858 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon2 | 8522979 | 8523098 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS2 | 8522979 | 8523098 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon3 | 8523185 | 8523445 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS3 | 8523185 | 8523445 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon4 | 8523498 | 8523867 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS4 | 8523498 | 8523867 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon5 | 8524764 | 8524894 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS5 | 8524764 | 8524894 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon6 | 8524952 | 8525068 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS6 | 8524952 | 8525068 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon7 | 8525122 | 8525376 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS7 | 8525122 | 8525376 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon8 | 8525433 | 8525573 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS8 | 8525433 | 8525573 |
| chr_1 | g10249 | g10249.t1 | exon | g10249.t1.exon9 | 8525634 | 8525843 |
| chr_1 | g10249 | g10249.t1 | cds | g10249.t1.CDS9 | 8525634 | 8525843 |
| chr_1 | g10249 | g10249.t1 | TTS | g10249.t1 | NA | NA |
Sequences
>g10249.t1 Gene=g10249 Length=1929
ATGTCTTTTATTAACGAGAACGAATTTTGTTGTGACAATGATTCTCATTCACAAATGAAT
ATTAACGGTTCGATAACATCAATCCATCATCATATGTCGTTTTTTGAAAGTGAACAATGG
ATAAAACGACATGAACGTGTAAATGTAATTTTTTTAGTGGCTGAATTTATTATAATGATT
ATTGCAATATTGGGAAATTCATTAGTTATAATAGTGTTTATTAAAAATCGAAAATTGCGT
AGAAAAACTAATTATTACATTGTATCTCTGTGTTTTGCCGATTTTTTGGTAGGCATCATT
GGCATTCCATTTTGTTTATTGTTGGTATCAACAAAACTTCCATCATCAAATTTTTTATTA
TGCCTGCTCCCGATCTCGACTCTCTTAACATTTTGTAATATATCAATTTTTAGTCTGTTT
TTGATTTCCATCGATCGCATTTTCTCAATAAACCTTCCTTTAAATTCAACGCTAAATTCA
ACTACAAAAAATGTAATGCTGGAAATAGCTTTTGTTTGGACATCTGGAATTTTTGTCGGT
TTTTTACCGATATTCTGGCATAAAGTACCACTTAATGATTCATGCATGCTTTATAATGTT
TTGGCTGATGGCTTTCAAATTTTTCGATTTATTTTTGTAATTCTTATTCCGGCATGCACA
ATGTTTGCGATTTATTGTAATATTTATAGAATAATTATCATACAGTTAAAAAATGAAGCT
ATTTTAAAAAGGCCACCGATCTCGTGTTGCGAAAAATCCAAAGAATCACTAGCTGTTTAT
CACACTTCTCAGCGCGTGCTTTTAAAACGAGAACTGAAAGCCACAGTCAACATATCTTTA
ATAATATTTATTTTTATCATTACATGGTTGCCGTTACATATAGTGAACCTTGTGACGATT
TTTTGTAAATCGTGTTTTATAAGTCGTGAAACTTTTTTACTTGTGTTAGCTATTGCACAC
AGTTCATCATCTTTGGATCCGCTGATTTATGCTTATCATATGAGAGATATAAGAAAATCT
ATACTTAAATTACTTGGTCTACAGATTCCTAAAACTTTGAACAGTAGACAATCAAGAAAC
ACATTCATCTTAATTGTGTTCTTCAAAGATAAAAATTTACAAAAAAGAAAAATAAATTTT
TTATTTGCATCACAAGCATTGGCTGATATTTTTGTTGCATCTATTGGAATTCCTTTGATT
TTATTATTATATACTGGTGTAATTAAGGAATACAAATTATGCACGTTTACAATTTCGACT
GTGATAGCAACACGCATTATTCCTATCAATAATATTGTTGCAATATCATATGATCGTTAT
AAGGCTCTTTCCAATTCAATTCAATATGCAATGACTAAACCATTCAAGTCAAAACATCCA
ATTTTATTGAGTTGGATTTTTGGAATTATCACAGGATATTTAATTTGTTTTTATAACACT
GGTGATGCTGAATTAGAATGCACATTTCGAAAGAAAATCACAAAAGAATACATGATTATT
CTTCAATTTATCTGCACAATAATTCCACTTTTGATAATATTCATCATTTACACATTAGTT
TATATCATGATGCGCAAAGTTTACATAAATCACTCATGCGATGAATGCACTTCTGATTCT
AAAATTTTGAAGAAAGCAAAATGTAAAATTAGGAGAAAAGAGTTTAGTGCAACAATCAAT
ATGCTTGCAATTGTAATAACTTTTGGCGTATGCAAATTGCCATTATTAATTATCCATTTA
ATTTATGTGTTTGGAAATCTACAACTTCACAAAGATATTCATTTTACTTTTATTGCAATT
AATCATTTGAACGCATTGATAAATCCTTTTTTGTATTCGTTTTTTCTAAAAGATTTTCGT
ACATCATGTAAAAAATTTTTAAATCTACCAGCTGAAAGAGAAAGCACAGCAATACATTCA
TCTCTTTAG
>g10249.t1 Gene=g10249 Length=642
MSFINENEFCCDNDSHSQMNINGSITSIHHHMSFFESEQWIKRHERVNVIFLVAEFIIMI
IAILGNSLVIIVFIKNRKLRRKTNYYIVSLCFADFLVGIIGIPFCLLLVSTKLPSSNFLL
CLLPISTLLTFCNISIFSLFLISIDRIFSINLPLNSTLNSTTKNVMLEIAFVWTSGIFVG
FLPIFWHKVPLNDSCMLYNVLADGFQIFRFIFVILIPACTMFAIYCNIYRIIIIQLKNEA
ILKRPPISCCEKSKESLAVYHTSQRVLLKRELKATVNISLIIFIFIITWLPLHIVNLVTI
FCKSCFISRETFLLVLAIAHSSSSLDPLIYAYHMRDIRKSILKLLGLQIPKTLNSRQSRN
TFILIVFFKDKNLQKRKINFLFASQALADIFVASIGIPLILLLYTGVIKEYKLCTFTIST
VIATRIIPINNIVAISYDRYKALSNSIQYAMTKPFKSKHPILLSWIFGIITGYLICFYNT
GDAELECTFRKKITKEYMIILQFICTIIPLLIIFIIYTLVYIMMRKVYINHSCDECTSDS
KILKKAKCKIRRKEFSATINMLAIVITFGVCKLPLLIIHLIYVFGNLQLHKDIHFTFIAI
NHLNALINPFLYSFFLKDFRTSCKKFLNLPAERESTAIHSSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 44 | g10249.t1 | CDD | cd00637 | 7tm_classA_rhodopsin-like | 360 | 615 | 5.32168E-20 |
| 16 | g10249.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 30 | 351 | 5.1E-55 |
| 15 | g10249.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 358 | 629 | 9.8E-39 |
| 4 | g10249.t1 | PANTHER | PTHR24247:SF239 | - | 48 | 344 | 3.9E-75 |
| 6 | g10249.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 48 | 344 | 3.9E-75 |
| 3 | g10249.t1 | PANTHER | PTHR24247:SF239 | - | 360 | 627 | 3.9E-75 |
| 5 | g10249.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 360 | 627 | 3.9E-75 |
| 11 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 50 | 74 | 3.6E-30 |
| 9 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 83 | 104 | 3.6E-30 |
| 12 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 128 | 150 | 3.6E-30 |
| 8 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 206 | 229 | 3.6E-30 |
| 10 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 274 | 298 | 3.6E-30 |
| 7 | g10249.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 312 | 338 | 3.6E-30 |
| 2 | g10249.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 65 | 330 | 4.2E-38 |
| 1 | g10249.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 360 | 612 | 2.4E-25 |
| 27 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 48 | - |
| 36 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 49 | 74 | - |
| 19 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 75 | 85 | - |
| 32 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 86 | 110 | - |
| 30 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 111 | 121 | - |
| 33 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 144 | - |
| 18 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 145 | 164 | - |
| 42 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 186 | - |
| 26 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 187 | 205 | - |
| 31 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 206 | 228 | - |
| 21 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 229 | 273 | - |
| 43 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 274 | 292 | - |
| 25 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 293 | 311 | - |
| 38 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 312 | 332 | - |
| 20 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 333 | 377 | - |
| 35 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 378 | 404 | - |
| 28 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 405 | 415 | - |
| 41 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 416 | 440 | - |
| 22 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 441 | 460 | - |
| 40 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 461 | 480 | - |
| 29 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 481 | 499 | - |
| 37 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 500 | 522 | - |
| 23 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 523 | 558 | - |
| 34 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 559 | 581 | - |
| 24 | g10249.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 582 | 592 | - |
| 39 | g10249.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 593 | 615 | - |
| 17 | g10249.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 616 | 642 | - |
| 59 | g10249.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 427 | 443 | - |
| 61 | g10249.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 65 | 330 | 35.337 |
| 60 | g10249.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 359 | 612 | 28.837 |
| 58 | g10249.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 59 | 345 | 6.2E-4 |
| 13 | g10249.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 33 | 358 | 2.93E-48 |
| 14 | g10249.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 360 | 629 | 1.01E-32 |
| 54 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 50 | 72 | - |
| 53 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 85 | 107 | - |
| 46 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 144 | - |
| 49 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 187 | - |
| 48 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 207 | 229 | - |
| 50 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 279 | 301 | - |
| 45 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 311 | 333 | - |
| 56 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 378 | 400 | - |
| 57 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 415 | 437 | - |
| 52 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 461 | 483 | - |
| 51 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 498 | 520 | - |
| 47 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 561 | 583 | - |
| 55 | g10249.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 593 | 615 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
