Gene loci information

Transcript annotation

  • This transcript has been annotated as Splicing factor 3B subunit 3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10306 g10306.t4 TTS g10306.t4 8868381 8868381
chr_1 g10306 g10306.t4 isoform g10306.t4 8868464 8869896
chr_1 g10306 g10306.t4 exon g10306.t4.exon1 8868464 8868474
chr_1 g10306 g10306.t4 exon g10306.t4.exon2 8868850 8869896
chr_1 g10306 g10306.t4 cds g10306.t4.CDS1 8868910 8869890
chr_1 g10306 g10306.t4 TSS g10306.t4 NA NA

Sequences

>g10306.t4 Gene=g10306 Length=1058
GTATCAATGTGTTTAGTTAAATTCAGCGTTGATGGTAAAAGTTATTTAGCATGCGGTGTT
GTTAAAGACATGCAGTTAAATCCACGACAAATTGGTTCTGGCTATATCGATTTATATCGA
GTCGATAATCAATGTGCAACGCTGAATTTGATTCATAGAACAGAAGTTGAAGATATTCCA
TCAGCAATAGCACCTTTTCAAGGTAAACTATTAGTTGGAGTTGGAAAGATTTTGCGAGTT
TATGATTTAGGCAAGAAAAAGTTATTAAGAAAATGCGAAAATAAGCACATCCCTAATCAA
ATTGTCAATATTCAAACGAGAGCTTTACGTGTATTTTGCTCAGATGTACAAGAAAGTGTT
TATTGTCTCACATACAAGCGAAATGAAAATCAATTGATTATTTTTGCTGATGACACTTTC
CCTCGATGGATAACAACAACAGCAATTTTGGATTATGATACAATAGCTACAGGTGACAAG
TTTGGAAATATTGCCATTTTGAGACTACCGCATTCTATCAATGATGATGTGGACGAAGAT
CCAACGGGAAACAAAGCACTTTGGGATCGAGGTTTGCTAAATGGAGCATCACAAAAAGCT
GAAAACATAATCACATTCCATATTGGTGAGATAGTAATGTCGCTACAAAAATCAATGTTG
ATTCCTGGTGGTCCAGAACTTTTAATTTATACAACAATTAGCGGTACAATTGGTGCATTA
GTTCCATTTACAGGAAGAGAAGATTTTGATTTCTTTCAACATTTAGAAATGCACATGAGA
AATGAAAATCCACCATTGTGTGGTAGAGATCATTTGAGTTATAGATCTTACTATTATCCA
GTAAAGAATGTCATTGATGGTGATTTGTGTGAACAGTTTACATCTTTAGATCCAGCAAAA
CAAAAGAGTATTGCATCGGATTTAAGTCGAAGTGCAACTGAGGTGGCAAAGAAGCTTGAA
GATATTAGAACAAGATACGCATTTTAAATTTTTTCACAGTCAAGTAAGTGTTTTATATGA
AAATAATTCTTTTTATGTAATTTTACTGTTTTCTTTAA

>g10306.t4 Gene=g10306 Length=326
MCLVKFSVDGKSYLACGVVKDMQLNPRQIGSGYIDLYRVDNQCATLNLIHRTEVEDIPSA
IAPFQGKLLVGVGKILRVYDLGKKKLLRKCENKHIPNQIVNIQTRALRVFCSDVQESVYC
LTYKRNENQLIIFADDTFPRWITTTAILDYDTIATGDKFGNIAILRLPHSINDDVDEDPT
GNKALWDRGLLNGASQKAENIITFHIGEIVMSLQKSMLIPGGPELLIYTTISGTIGALVP
FTGREDFDFFQHLEMHMRNENPPLCGRDHLSYRSYYYPVKNVIDGDLCEQFTSLDPAKQK
SIASDLSRSATEVAKKLEDIRTRYAF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
4 g10306.t4 Gene3D G3DSA:2.130.10.10 - 1 240 0.0e+00
5 g10306.t4 Gene3D G3DSA:3.30.980.30 - 244 323 5.3e-06
2 g10306.t4 PANTHER PTHR10644 DNA REPAIR/RNA PROCESSING CPSF FAMILY 1 319 0.0e+00
3 g10306.t4 PANTHER PTHR10644:SF1 SPLICING FACTOR 3B SUBUNIT 3 1 319 0.0e+00
1 g10306.t4 Pfam PF03178 CPSF A subunit region 1 292 0.0e+00

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005634 nucleus CC
GO:0005515 protein binding MF
GO:0003676 nucleic acid binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values