General information for g10326.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative lipoyltransferase 2, mitochondrial.
Parent gene
- The parent gene g10326 is located on chr_1: 8922274..8923044 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10326 | g10326.t1 | TTS | g10326.t1 | 8922253 | 8922253 |
| chr_1 | g10326 | g10326.t1 | isoform | g10326.t1 | 8922274 | 8923044 |
| chr_1 | g10326 | g10326.t1 | exon | g10326.t1.exon1 | 8922274 | 8922818 |
| chr_1 | g10326 | g10326.t1 | cds | g10326.t1.CDS1 | 8922274 | 8922818 |
| chr_1 | g10326 | g10326.t1 | exon | g10326.t1.exon2 | 8922885 | 8923044 |
| chr_1 | g10326 | g10326.t1 | cds | g10326.t1.CDS2 | 8922885 | 8923044 |
| chr_1 | g10326 | g10326.t1 | TSS | g10326.t1 | 8923070 | 8923070 |
Sequences
>g10326.t1 Gene=g10326 Length=705
ATGAATTCAACAAAACTTATTCACATTTTTAATGTGGGTAAATTGCCATATTTAGAATCT
CTTAATCTTCAAAAATTTATCTCAACACAAATAATTCAAAATACTTGGAATTATAAAAAT
GTTTTAATTTTAACTGAACATTATCCAGTTTATACAATAGGAATCAGGACTAAAAATTAT
ACTATAGAAGATGAAAATAAGTTGAGAAACTTAGGTGCAGAGTTTATCAAGACAAATAGA
GGAGGTCTTATTACATTTCATGGACCTGGACAGCTTGTTGCATATCCAATTATCAATTTA
AAAAATTTCAAGCAAGGTGTTCGTTGGTACGTGAATAGTATTGAAAGAACGATAATAAAT
TTATGCCATAAATATGAATTAAACGCAACCACTACAACTGATACGGGTGTATGGATTAAT
GATAGAAAGATTTGTGCTATTGGCATTCATGTTAGTCGATATGTGACAATGCATGGACTT
GCATTGAATTGTAACACTGATTTAAGTTGGTTCAATCATATTGTACCTTGTGGAATCGAA
GACAAAGAAGTTACATCTCTTTCTAAAGAATGCAAAAGAGACATTCAAATAAATGATGTT
ATTCCAGAATTAATTGCAAGCTTCAGCACAATTTTTGAAAGCAAAACTATTTTAGTTAAT
CAAGAACAAGTTGATGAGATTAAAAAAAATTCTTTAGAGAAATAA
>g10326.t1 Gene=g10326 Length=234
MNSTKLIHIFNVGKLPYLESLNLQKFISTQIIQNTWNYKNVLILTEHYPVYTIGIRTKNY
TIEDENKLRNLGAEFIKTNRGGLITFHGPGQLVAYPIINLKNFKQGVRWYVNSIERTIIN
LCHKYELNATTTTDTGVWINDRKICAIGIHVSRYVTMHGLALNCNTDLSWFNHIVPCGIE
DKEVTSLSKECKRDIQINDVIPELIASFSTIFESKTILVNQEQVDEIKKNSLEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10326.t1 | CDD | cd16444 | LipB | 8 | 212 | 1.80449E-99 |
| 6 | g10326.t1 | Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | 6 | 213 | 4.4E-77 |
| 4 | g10326.t1 | Hamap | MF_00013 | Octanoyltransferase [lipB]. | 6 | 215 | 30.756613 |
| 2 | g10326.t1 | PANTHER | PTHR10993:SF7 | LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED | 6 | 220 | 8.5E-75 |
| 3 | g10326.t1 | PANTHER | PTHR10993 | OCTANOYLTRANSFERASE | 6 | 220 | 8.5E-75 |
| 7 | g10326.t1 | PIRSF | PIRSF016262 | LPLase | 1 | 227 | 3.8E-67 |
| 1 | g10326.t1 | Pfam | PF03099 | Biotin/lipoate A/B protein ligase family | 65 | 167 | 9.9E-10 |
| 9 | g10326.t1 | ProSitePatterns | PS01313 | Lipoate-protein ligase B signature. | 80 | 95 | - |
| 11 | g10326.t1 | ProSiteProfiles | PS51733 | Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) catalytic domain profile. | 36 | 216 | 60.504 |
| 5 | g10326.t1 | SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | 7 | 213 | 1.14E-61 |
| 10 | g10326.t1 | TIGRFAM | TIGR00214 | lipB: lipoyl(octanoyl) transferase | 30 | 212 | 2.3E-54 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0033819 | lipoyl(octanoyl) transferase activity | MF |
| GO:0006464 | cellular protein modification process | BP |
| GO:0009249 | protein lipoylation | BP |
KEGG
Orthology
- This transcript has been annotated with K23735 (lipoyl(octanoyl) transferase 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
