Gene loci information

Transcript annotation

  • This transcript has been annotated as Ubiquitin-conjugating enzyme E2 N.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10333 g10333.t4 TSS g10333.t4 8931397 8931397
chr_1 g10333 g10333.t4 isoform g10333.t4 8931467 8932825
chr_1 g10333 g10333.t4 exon g10333.t4.exon1 8931467 8931475
chr_1 g10333 g10333.t4 exon g10333.t4.exon2 8931543 8931660
chr_1 g10333 g10333.t4 exon g10333.t4.exon3 8931727 8932825
chr_1 g10333 g10333.t4 cds g10333.t4.CDS1 8931805 8932263
chr_1 g10333 g10333.t4 TTS g10333.t4 8933021 8933021

Sequences

>g10333.t4 Gene=g10333 Length=1226
TCTTGCGTGCAAGTCTCAAATAGGAAATTCATAGGAAAAATTGCCAACAAAGAATTTACT
TTGGATTGAAAAAAAATTAACAAAGCCATCCAGTGCAAACAAGAAGGGAAGAAAATCGAA
TAAAGTGTAAGCGAGCGTTAAAGGTTTATTTTAAAGACTTAAATAATTCAGCAAAGGAAA
AAAGCAAAATATTGCAACAAAAACAATGGCTGCATTGCCGAGAAGAATTATCAAAGAAAC
TCAACGATTAATGCAAGAACCAGTTCCGGGAATATCTGCAGTTCCCGATGAGAGCAATGC
TCGCTATTTCCATGTAATTGTCACTGGCCCAGAAGACTCTCCATTCGAAGGCGGTCTCTT
TAAATTGGAATTGTTCCTGCCAGAAGATTATCCTATGTCTGCGCCTAAAGTTCGTTTTAT
AACAAAAATTTATCATCCAAATATCGATAGACTAGGTAGAATCTGTTTAGACATTCTCAA
AGACAAATGGTCGCCGGCTCTGCAAATTCGCACTGTTTTGTTGTCGATTCAGGCCTTATT
AAGTGCTCCAAATCCTGATGATCCTCTTGCAAATGATGTTGCTGAATTGTGGAAGGTGAA
TGAGGCTGAAGCAATACGAAATGCCAAAGAATGGACACAACGGTATGCAATGGAGGATAA
TTAAAAGGAGTGCGAATGAAATACTAAATAATTAGAAACAGCAGCATCAATATAATCATC
ATTGCTATGTATGAATTGTTGCAAAAAGAAAAAAAAAACATGCAAAATTATCATTAATTT
TTATTATTTGAGAACGAGGAAAAAATCATTATTTTATCACTCTTAAATTTTTTATTTATT
GCCACCTATTCTTCACACACACACACATGTTACTTTTTTTTTCTTCTCTACTCTCTTAAA
AGTTCTCTTAAATGACACATATTTTTTGGTAGTGGATTTTTACTACTTTTGTGGTAGGTT
TTGATTTTGACACACATTGACACATATGAGATAAATTCCTCGTTTGAGAAAATGATTACA
TGGGATTACATATAAAAAATTAGCAAAAATATTAATTGCAATTGATTCTCATTTTATCAT
GTGTTTTAAAACAATGCATTGTCCTATTTTACCTACCAATCTACTTACTTTACTTATATA
TACATAGCTATTCTTATTACTTTGCTAAAATCAATGATGATATGATGATTTCTTAAGTTG
AAAAAATATGGTTTATTAATGAAGAA

>g10333.t4 Gene=g10333 Length=152
MAALPRRIIKETQRLMQEPVPGISAVPDESNARYFHVIVTGPEDSPFEGGLFKLELFLPE
DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP
LANDVAELWKVNEAEAIRNAKEWTQRYAMEDN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g10333.t4 CDD cd00195 UBCc 5 144 7.42509E-66
5 g10333.t4 Gene3D G3DSA:3.10.110.10 Ubiquitin Conjugating Enzyme 1 152 4.8E-68
2 g10333.t4 PANTHER PTHR24068:SF193 UBIQUITIN-CONJUGATING ENZYME E2 N-LIKE 2 148 4.1E-78
3 g10333.t4 PANTHER PTHR24068 UBIQUITIN-CONJUGATING ENZYME E2 2 148 4.1E-78
1 g10333.t4 Pfam PF00179 Ubiquitin-conjugating enzyme 7 143 2.8E-47
7 g10333.t4 ProSitePatterns PS00183 Ubiquitin-conjugating enzymes active site. 76 91 -
9 g10333.t4 ProSiteProfiles PS50127 Ubiquitin-conjugating enzymes family profile. 6 138 41.715
8 g10333.t4 SMART SM00212 ubc_7 6 149 2.2E-70
4 g10333.t4 SUPERFAMILY SSF54495 UBC-like 5 149 3.28E-56

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values