General information for g10334.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein disulfide-isomerase A3.
Parent gene
- The parent gene g10334 is located on chr_1: 8934649..8935590 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10334 | g10334.t10 | TTS | g10334.t10 | 8933125 | 8933125 |
| chr_1 | g10334 | g10334.t10 | isoform | g10334.t10 | 8933413 | 8934919 |
| chr_1 | g10334 | g10334.t10 | exon | g10334.t10.exon1 | 8933413 | 8933710 |
| chr_1 | g10334 | g10334.t10 | cds | g10334.t10.CDS1 | 8933413 | 8933710 |
| chr_1 | g10334 | g10334.t10 | exon | g10334.t10.exon2 | 8933766 | 8933942 |
| chr_1 | g10334 | g10334.t10 | cds | g10334.t10.CDS2 | 8933766 | 8933942 |
| chr_1 | g10334 | g10334.t10 | exon | g10334.t10.exon3 | 8934000 | 8934407 |
| chr_1 | g10334 | g10334.t10 | cds | g10334.t10.CDS3 | 8934000 | 8934407 |
| chr_1 | g10334 | g10334.t10 | exon | g10334.t10.exon4 | 8934468 | 8934696 |
| chr_1 | g10334 | g10334.t10 | cds | g10334.t10.CDS4 | 8934468 | 8934679 |
| chr_1 | g10334 | g10334.t10 | exon | g10334.t10.exon5 | 8934759 | 8934919 |
| chr_1 | g10334 | g10334.t10 | TSS | g10334.t10 | 8935652 | 8935652 |
Sequences
>g10334.t10 Gene=g10334 Length=1273
TCGGTAAGTTTCGAGATTTCAAAATTGAAAGTTGATTAAAAACAGTTGAAATTATTAATT
TGCTTTATATTTAAAATCGCAAGGTGTTTCTGGATATCCCACTCTCAAAATTTTCAGAAA
CGGTGAAAATCCTCAGGATTATAATGGTCCACGTGAAGCAAGTGGTATTGTGAAATATAT
GAGAGCACAAGTTGGTCCATCATCAAAGGATCTTCTAACAGTTGAAGCATATGAAAAGTT
CTTGGGTGTTCAAGAGGGTGCCGTAGTTGGATTCTTTGAAAAAGAAACGGATTTGAAGAC
TGTTTTCTTAAAATATGCCGATTTGCAACGTGAGAAACTTCGCTTTGCACATTCAAGTGA
TCCAGAAATTCTCAAGAAAGTTGGCGAAACAAACGCTATTCTTCTTTATCGTGCACCACA
ATTGAGTAATAAGTTTGAACCATCTTTTGTTAAATTCACTGGAAAAGATTCAAGTGATCT
TAAAGAATTTGTTAAGAAGAACTTCCACGGTTTAGTTGGTGTACGTAGTCGTGATACTAT
TACTGATTTCAACAATCCATTGGTAACTGCATATTATGCTGTTGATTATATTAAAAATGC
AAAAGGCACAAACTACTGGCGCAATCGTGTACTTAAAGTTGCCAAAGAATGGGAAGGTAA
AATCAATTTTGCTATTGCTGCTAAAGATGAATTCCAACATGAATTGAATGAATATGGTTA
CGATTATGTCGGTGATAAACCAATAATTTTGGCTCGTGACGCTCAAAATCAAAAGTTCAT
CATGAAAGATGAGTTTTCTGTTGACAATCTTCAAGCATTTGTCAATGATCTTTATGAAGG
CAATCTGGAGCCTTACGTTAAATCAGAAGCAATTCCAGTCGATCAAGATGGCCCAGTTAC
AGTTGTAGTTGCAAAGAACTTTAATGAGGCTGTTCCAAAAGATAAGGATGTTTTAATCGA
ATTCTACGCACCATGGTGTGGTCATTGCAAAAAATTAGCTCCAACATTCGATGAATTAGG
AACAAAATTGAAGGGTGAAGATGTTGTTATTGCCAAAATGGATGCTACTGCAAACGATGT
TCCTCCTCAATTTGATGTTCGCGGATTCCCGACACTCTTCTGGTTGCCTAAGGGCGCTCA
TGATAAACCAGTTAGATACGATGGCGGTCGTGAATTGAACGATTTTATCAGCTACATCAG
TAAACACGCTACAAATGAATTAAAAGGATACGATCGCTCTGGAAACGAAAAGAAACAAAA
GACAGAATTGTAA
>g10334.t10 Gene=g10334 Length=364
MRAQVGPSSKDLLTVEAYEKFLGVQEGAVVGFFEKETDLKTVFLKYADLQREKLRFAHSS
DPEILKKVGETNAILLYRAPQLSNKFEPSFVKFTGKDSSDLKEFVKKNFHGLVGVRSRDT
ITDFNNPLVTAYYAVDYIKNAKGTNYWRNRVLKVAKEWEGKINFAIAAKDEFQHELNEYG
YDYVGDKPIILARDAQNQKFIMKDEFSVDNLQAFVNDLYEGNLEPYVKSEAIPVDQDGPV
TVVVAKNFNEAVPKDKDVLIEFYAPWCGHCKKLAPTFDELGTKLKGEDVVIAKMDATAND
VPPQFDVRGFPTLFWLPKGAHDKPVRYDGGRELNDFISYISKHATNELKGYDRSGNEKKQ
KTEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g10334.t10 | CDD | cd03073 | PDI_b’_ERp72_ERp57 | 113 | 218 | 2.92625E-38 |
| 15 | g10334.t10 | CDD | cd02995 | PDI_a_PDI_a’_C | 239 | 340 | 9.95179E-51 |
| 12 | g10334.t10 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 3 | 115 | 1.7E-30 |
| 13 | g10334.t10 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 118 | 221 | 1.1E-34 |
| 11 | g10334.t10 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 227 | 363 | 1.1E-40 |
| 3 | g10334.t10 | PANTHER | PTHR18929:SF60 | PROTEIN DISULFIDE-ISOMERASE | 2 | 348 | 4.5E-105 |
| 4 | g10334.t10 | PANTHER | PTHR18929 | PROTEIN DISULFIDE ISOMERASE | 2 | 348 | 4.5E-105 |
| 7 | g10334.t10 | PRINTS | PR00421 | Thioredoxin family signature | 258 | 266 | 3.7E-6 |
| 6 | g10334.t10 | PRINTS | PR00421 | Thioredoxin family signature | 266 | 275 | 3.7E-6 |
| 5 | g10334.t10 | PRINTS | PR00421 | Thioredoxin family signature | 306 | 317 | 3.7E-6 |
| 1 | g10334.t10 | Pfam | PF13848 | Thioredoxin-like domain | 34 | 217 | 1.8E-32 |
| 2 | g10334.t10 | Pfam | PF00085 | Thioredoxin | 240 | 341 | 7.7E-28 |
| 16 | g10334.t10 | ProSitePatterns | PS00194 | Thioredoxin family active site. | 259 | 277 | - |
| 18 | g10334.t10 | ProSiteProfiles | PS51352 | Thioredoxin domain profile. | 218 | 345 | 15.679 |
| 8 | g10334.t10 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 10 | 109 | 5.47E-12 |
| 10 | g10334.t10 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 111 | 237 | 5.18E-17 |
| 9 | g10334.t10 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 204 | 344 | 2.34E-33 |
| 17 | g10334.t10 | TIGRFAM | TIGR01126 | pdi_dom: protein disulfide-isomerase domain | 243 | 343 | 1.2E-34 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003756 | protein disulfide isomerase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
