General information for g10381.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative 5-hydroxytryptamine receptor 2A.
Parent gene
- The parent gene g10381 is located on chr_1: 9342587..9346944 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10381 | g10381.t1 | isoform | g10381.t1 | 9342587 | 9346944 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon1 | 9342587 | 9343742 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS1 | 9342587 | 9343742 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon2 | 9344598 | 9344822 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS2 | 9344598 | 9344822 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon3 | 9344908 | 9345049 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS3 | 9344908 | 9345049 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon4 | 9345204 | 9345435 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS4 | 9345204 | 9345435 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon5 | 9345519 | 9345751 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS5 | 9345519 | 9345751 |
| chr_1 | g10381 | g10381.t1 | exon | g10381.t1.exon6 | 9346500 | 9346944 |
| chr_1 | g10381 | g10381.t1 | cds | g10381.t1.CDS6 | 9346500 | 9346944 |
| chr_1 | g10381 | g10381.t1 | TSS | g10381.t1 | NA | NA |
| chr_1 | g10381 | g10381.t1 | TTS | g10381.t1 | NA | NA |
Sequences
>g10381.t1 Gene=g10381 Length=2433
ATGATGAAAGAAGAAGTGATAGAGCTATATGATAGCAATGACTTCTCATCAATGGTGCTT
TCAACGATCAACCTTCATAAACAGAATATTTTTACTTATAATCAGACAAATCATAGTATT
GCATATGATAATGAGACAGAAAATAATGAATTTAACAAATATTTAAATAACAACTCTGTT
TATGATGATAATTTTACATTTCAACAAGACGACAAAAGTTCGAAGATCATTGACCTAGAA
TATGTAGAAATAAATAATTACTGGGCACTTTTAGCACTGATATTAGTTTTTGGAACCGCT
GGCGGCAATATATTAGTTTGTCTTGCAATTGCATGGGAAAAGCGTCTACAAAATGTTACC
AATTATTTCCTAGCGTCATTAGCTATAACAGATTTAATGGTTGCTGTTCTTGTAATGCCT
TTAGGGATTGTAACGTTATACATGGGATATTTCCCATTACAATCAGTACATTGTTTAGTC
TGGATATGTTTGGACGTTCTCTTTTGTACAGCAAGTATCATGCACTTATGCACAATATCA
ATTGATCGATATCTATCATTACGATATCCTATGAGATTTGGACGAAATAAAACACGTAGA
AGAGTTGTTCTCAAAATCTTTTTTGTATGGTTATTGTCAATTGCAATGAGTCTACCATTG
AGCTTGATGTATTCAAAGAATCATGCATCTGTGCTAGTAGACGGAACATGCCAAATTCCT
GACCCTGTATACAAACTCGTTGGATCGATTGTATGTTTTTATATTCCTTTGGGTGTAATG
CTTCTAACTTACACCTTAACCGTTCGATTATTGGCGCGTCAAAGGCAAAATTTGGGCGGA
AGTGGATGCGGAATAAAAAGCACAAGCGGCTGGTCTAGTGGATGGCTCGGTATAGCTGCG
CCTCCATTAGCAGAGAGGAAAAATACATGGAAGCGGTTCATAAAAAGCAGTATACCATCG
ACACCGAATCATGCTCATTCTGCTGGCTCAACAGACACCGAGCTGTCGACTTTAGATATA
AATACACATGAACTATGGTTACCCGAGTCGAGCATACCAGAACCGACACCATCGACCATG
ACAGCATTACATCAGTTTGGTGCAGAAATGCTGAAGCTCTCAAGAGGCCTAGAATCATTT
ACTCCCATCAATGAACCGAAAAATGCTGATATTATTGAACCCTCGAAGGCAAATGATGAA
GATGATGACGTCTCAAATATTAATAAGTTTGAACCACCATCGTCAACCGATTTACAGACT
GAAATAGCAGCAAAGCGCACAACAACAAACGTTACAGCTAATAATAGAAAAAGACTTCCT
CAATTAACCAGAGTTACGACTAAACCACAAAAGAGAAGAGCATCACTAAGTGTTTTCCAC
AATAAAAAATCCTCGACACGTCTTCGGCGTTCTAATAGCATACAAACATTTGAACACGAT
GAACGTAGTGATAGTACAGATGGCAATTTTTTTGATGAAATTAATCAACTGAAGCACAGT
AACAAAAAGAAGAAACAAAAAAGTGGAAAGAATGACAGAGATACAATTTCATTAGAACTG
CCACCTCCATGTACTTGTCCTTATTTTGGTGATAAACCATGCGCAATGGTAAAACCAACT
GAAGTTAAAATCATAACAACATCCTCAAGCAATTATTTTGTACCAGAAAACAAACAACAT
CAATTTCATGACTCAAATCAATTTATCTCGTCATTATCGAGTAACAATTCCATATCAAAT
ACGACATCAACTAATAGCTTGCCACTAGCACAGGGTACAATTAAACGTGGACGACAAAAT
TATAGTACTAGCAGCAATAATGCTATAACAAAATGCACTTCTGTAGTAACATGGGACTCA
AGGCGTTATCAAAGACGAGGATCTTCGATAAGTGGTGCTAAAACAACACTCGTTCTGACA
CCTAACAAATCATCACTTGCAGCAACGCCACTTCGTAGATCTGTTACGCTCCGTCATAAT
ACAAACCTCAATTCAACCAATGATACAGAAAGTATTGCAGCGCACAAAGTTCCATCATCA
CCATGTATTCTTCAGAGAACTACAACAATTCGATCACATCATTCACGCAATTCAAGTGTT
ATTTCGCGAAATTCATCACGTCATGGACGCATCATCCGACTAGAGCAAAAAGCAACAAAA
GTATTGGGTGTTGTTTTTTTCACTTTTGTGATTTTATGGGCACCTTTTTTTGTACTGAAT
CTTCTACCTACTGTATGTCCAGACTGTGAATCGAATATAAGCCACGGAGTTTTTGATTTC
GTTACATGGCTCGGTTACGCTTCTTCAATGGTTAATCCAATTTTTTATACAATCTTCAAT
AAGGTATTTCGACAGGCATTCAAAAAAGTTTTGCTGTGTCGCTATAAGAGTAAAGTTATA
TGGCGTCCCTTACCAATAAACCAACATCGGTAG
>g10381.t1 Gene=g10381 Length=810
MMKEEVIELYDSNDFSSMVLSTINLHKQNIFTYNQTNHSIAYDNETENNEFNKYLNNNSV
YDDNFTFQQDDKSSKIIDLEYVEINNYWALLALILVFGTAGGNILVCLAIAWEKRLQNVT
NYFLASLAITDLMVAVLVMPLGIVTLYMGYFPLQSVHCLVWICLDVLFCTASIMHLCTIS
IDRYLSLRYPMRFGRNKTRRRVVLKIFFVWLLSIAMSLPLSLMYSKNHASVLVDGTCQIP
DPVYKLVGSIVCFYIPLGVMLLTYTLTVRLLARQRQNLGGSGCGIKSTSGWSSGWLGIAA
PPLAERKNTWKRFIKSSIPSTPNHAHSAGSTDTELSTLDINTHELWLPESSIPEPTPSTM
TALHQFGAEMLKLSRGLESFTPINEPKNADIIEPSKANDEDDDVSNINKFEPPSSTDLQT
EIAAKRTTTNVTANNRKRLPQLTRVTTKPQKRRASLSVFHNKKSSTRLRRSNSIQTFEHD
ERSDSTDGNFFDEINQLKHSNKKKKQKSGKNDRDTISLELPPPCTCPYFGDKPCAMVKPT
EVKIITTSSSNYFVPENKQHQFHDSNQFISSLSSNNSISNTTSTNSLPLAQGTIKRGRQN
YSTSSNNAITKCTSVVTWDSRRYQRRGSSISGAKTTLVLTPNKSSLAATPLRRSVTLRHN
TNLNSTNDTESIAAHKVPSSPCILQRTTTIRSHHSRNSSVISRNSSRHGRIIRLEQKATK
VLGVVFFTFVILWAPFFVLNLLPTVCPDCESNISHGVFDFVTWLGYASSMVNPIFYTIFN
KVFRQAFKKVLLCRYKSKVIWRPLPINQHR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g10381.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 82 | 288 | 2.3E-53 |
| 12 | g10381.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 663 | 797 | 2.1E-28 |
| 40 | g10381.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 385 | 417 | - |
| 37 | g10381.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 392 | 407 | - |
| 39 | g10381.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 446 | 488 | - |
| 38 | g10381.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 473 | 488 | - |
| 2 | g10381.t1 | PANTHER | PTHR24247:SF222 | 5-HYDROXYTRYPTAMINE (SEROTONIN) RECEPTOR 2B, ISOFORM E | 83 | 797 | 5.7E-121 |
| 3 | g10381.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 83 | 797 | 5.7E-121 |
| 10 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 87 | 111 | 1.9E-41 |
| 6 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 120 | 141 | 1.9E-41 |
| 9 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 165 | 187 | 1.9E-41 |
| 5 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 201 | 222 | 1.9E-41 |
| 4 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 245 | 268 | 1.9E-41 |
| 7 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 718 | 742 | 1.9E-41 |
| 8 | g10381.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 758 | 784 | 1.9E-41 |
| 1 | g10381.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 102 | 776 | 4.4E-64 |
| 20 | g10381.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 86 | - |
| 23 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 110 | - |
| 17 | g10381.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 111 | 121 | - |
| 28 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 147 | - |
| 19 | g10381.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 148 | 158 | - |
| 26 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 159 | 181 | - |
| 15 | g10381.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 182 | 201 | - |
| 27 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 202 | 224 | - |
| 21 | g10381.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 225 | 243 | - |
| 22 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 244 | 266 | - |
| 14 | g10381.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 267 | 720 | - |
| 25 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 721 | 740 | - |
| 18 | g10381.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 741 | 759 | - |
| 24 | g10381.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 760 | 779 | - |
| 16 | g10381.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 780 | 810 | - |
| 36 | g10381.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 171 | 187 | - |
| 41 | g10381.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 102 | 776 | 35.156 |
| 11 | g10381.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 81 | 798 | 1.92E-62 |
| 29 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 109 | - |
| 30 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 144 | - |
| 34 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 159 | 181 | - |
| 35 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 202 | 224 | - |
| 33 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 246 | 268 | - |
| 32 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 723 | 745 | - |
| 31 | g10381.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 760 | 779 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04157 (5-hydroxytryptamine receptor 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
