General information for g10478.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutathione synthetase.
Parent gene
- The parent gene g10478 is located on chr_1: 10098472..10101482 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10478 | g10478.t2 | TTS | g10478.t2 | 10098330 | 10098330 |
| chr_1 | g10478 | g10478.t2 | isoform | g10478.t2 | 10098472 | 10101482 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon1 | 10098472 | 10098530 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS1 | 10098472 | 10098530 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon2 | 10098602 | 10098752 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS2 | 10098602 | 10098752 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon3 | 10098806 | 10099504 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS3 | 10098806 | 10099504 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon4 | 10099576 | 10099618 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS4 | 10099576 | 10099618 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon5 | 10100652 | 10100836 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS5 | 10100652 | 10100836 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon6 | 10101004 | 10101216 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS6 | 10101004 | 10101216 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon7 | 10101270 | 10101330 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS7 | 10101270 | 10101330 |
| chr_1 | g10478 | g10478.t2 | exon | g10478.t2.exon8 | 10101394 | 10101482 |
| chr_1 | g10478 | g10478.t2 | cds | g10478.t2.CDS8 | 10101394 | 10101482 |
| chr_1 | g10478 | g10478.t2 | TSS | g10478.t2 | 10101611 | 10101611 |
Sequences
>g10478.t2 Gene=g10478 Length=1500
ATGAGTTATCAAAAAGTTCTACCATCAGCCATTGATTTGCCATTAAATCAAAAAGAACTT
GGTGAAATTATTTCGAAGGCTAAAGATTGGGCATTGATGCATGGGGTCTGTATGCGTTCA
AAGACTAACTTTAGTGCTGATGCATTACAGTTTGCACCATTTGTATTAACTCCATCTTTG
TATCCAAGAAAAGAATTTGAAAAAGCTGTGAATCTCCAAACAGTGTTAAATGAGTTAATG
CATAAAGTTGCTCATGACACTGATTTTTTGAGAGAAACTCTCGCAAGTACTATCCAAGTA
GATGAATTTACCGCTAATTTGTTCAAGATTTACGAAACTGTTTTAAATGAAGGATTTACA
CAGGAAATATCACTTGGTTTAATTCGATCGGATACAATGCTAGAGACAAAGAAAGGCTGT
ATAGAGAAGTGCAAAAATTTAAATCCAAATTGTCCTAAAAGATACTACTGTTGCGTTGCA
ATGGTTGAAATCAATACTATTGCTTCAGGTTTTGGCCATTTGGGACCAGCATCAAGAGCC
ATTCAAAGTTACATATTGAACGAGCTTGGTCATGTCGATAAGACTAAAAATCTGCCAGAG
AATAATGCACTATCTGGATTGTGTTCAGCTATGGTTGAAGCTTGGAATATAATTGGAAAT
CAAAAAGCTGCCATTCTATTTGTGATTGAAGATGTTACTTATAATATTTGTGATCAGCGT
TTTCATGAATTTGAAATACGTGAAAAGTATCCACATGTTAAAGTCATTCGTCGGACTTTG
ACAGAAATTTTCAATACTGGTGTATTGGGATCTAATAAAGAACTAATTGTCGATGGCATT
GTTGTAAGTGTTGTATATTTCCGTTCTGGTTATGAGCCAAATCAATATCCATCGAAAAAT
GAATGGGATGCAAGACTTATGATTGAGCGATCAAATGCAATAAAGTGTCCTTCTATACAT
TATCATCTTGCCGGAACAAAAAAAGTGCAACAAGCTCTTGCTAAAAAAGGAGTTCTAAAT
AGATTTCTTTCAAATGAGACAGATATACAAAAAGTTCAAGAGATTTTTACAGGACTTTAT
TCTCTTGATAAAAAAGAAGGCGGAGATGAAGTTGTTGATATGGTACTTAAAAATCCTGAG
AGTTTTGTTATGAAACCGCAACGAGAAGGCGGTGGAAATAATATTTATGGAGTTGATATT
CCAGAAAAACTTACAGCTATGAGCGATGACGAACGATCAGCTTATATTGTTATGGATCGT
ATTTTTCCACCAATCTCTAAAAGTTATATGATAAGACCAGGTGGACAAATGCAACCTGAA
ATTGTCGAAATGGTGAGTGAGCTTGGGATTTTTGGAGCAATAATTGGAACAAAAGATAAA
ATTTTATACAATCGTCAAGTCGGACACATGCTGAGAACAAAAGTGTCTAACGCTAATGAA
GGTGGAGTAGCTGCTGGTTTAGGTGCTCTTGACAGTCCATATCTCTTTGATTCAGAATAA
>g10478.t2 Gene=g10478 Length=499
MSYQKVLPSAIDLPLNQKELGEIISKAKDWALMHGVCMRSKTNFSADALQFAPFVLTPSL
YPRKEFEKAVNLQTVLNELMHKVAHDTDFLRETLASTIQVDEFTANLFKIYETVLNEGFT
QEISLGLIRSDTMLETKKGCIEKCKNLNPNCPKRYYCCVAMVEINTIASGFGHLGPASRA
IQSYILNELGHVDKTKNLPENNALSGLCSAMVEAWNIIGNQKAAILFVIEDVTYNICDQR
FHEFEIREKYPHVKVIRRTLTEIFNTGVLGSNKELIVDGIVVSVVYFRSGYEPNQYPSKN
EWDARLMIERSNAIKCPSIHYHLAGTKKVQQALAKKGVLNRFLSNETDIQKVQEIFTGLY
SLDKKEGGDEVVDMVLKNPESFVMKPQREGGGNNIYGVDIPEKLTAMSDDERSAYIVMDR
IFPPISKSYMIRPGGQMQPEIVEMVSELGIFGAIIGTKDKILYNRQVGHMLRTKVSNANE
GGVAAGLGALDSPYLFDSE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g10478.t2 | Gene3D | G3DSA:3.30.1490.80 | - | 20 | 439 | 0 |
| 7 | g10478.t2 | Gene3D | G3DSA:3.30.470.20 | - | 60 | 493 | 0 |
| 8 | g10478.t2 | Gene3D | G3DSA:1.10.1080.10 | Glutathione Synthetase; Chain A | 72 | 358 | 0 |
| 9 | g10478.t2 | Gene3D | G3DSA:3.40.50.1760 | - | 201 | 325 | 0 |
| 11 | g10478.t2 | Gene3D | G3DSA:3.30.1490.50 | - | 359 | 425 | 0 |
| 3 | g10478.t2 | PANTHER | PTHR11130:SF0 | GLUTATHIONE SYNTHETASE | 11 | 495 | 0 |
| 4 | g10478.t2 | PANTHER | PTHR11130 | GLUTATHIONE SYNTHETASE | 11 | 495 | 0 |
| 13 | g10478.t2 | PIRSF | PIRSF001558 | GSHase | 2 | 496 | 0 |
| 1 | g10478.t2 | Pfam | PF03917 | Eukaryotic glutathione synthase, ATP binding domain | 18 | 495 | 0 |
| 2 | g10478.t2 | Pfam | PF03199 | Eukaryotic glutathione synthase | 224 | 324 | 0 |
| 6 | g10478.t2 | SUPERFAMILY | SSF56059 | Glutathione synthetase ATP-binding domain-like | 12 | 495 | 0 |
| 5 | g10478.t2 | SUPERFAMILY | SSF52440 | PreATP-grasp domain | 222 | 326 | 0 |
| 12 | g10478.t2 | TIGRFAM | TIGR01986 | glut_syn_euk: glutathione synthetase | 21 | 495 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004363 | glutathione synthase activity | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
| GO:0016874 | ligase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K21456 (glutathione synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
