General information for g10490.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Chitooligosaccharidolytic beta-N-acetylglucosaminidase.
Parent gene
- The parent gene g10490 is located on chr_1: 10152746..10154901 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10490 | g10490.t1 | TSS | g10490.t1 | 10152724 | 10152724 |
| chr_1 | g10490 | g10490.t1 | isoform | g10490.t1 | 10152746 | 10154901 |
| chr_1 | g10490 | g10490.t1 | exon | g10490.t1.exon1 | 10152746 | 10152819 |
| chr_1 | g10490 | g10490.t1 | cds | g10490.t1.CDS1 | 10152746 | 10152819 |
| chr_1 | g10490 | g10490.t1 | exon | g10490.t1.exon2 | 10153046 | 10153968 |
| chr_1 | g10490 | g10490.t1 | cds | g10490.t1.CDS2 | 10153046 | 10153968 |
| chr_1 | g10490 | g10490.t1 | exon | g10490.t1.exon3 | 10154034 | 10154687 |
| chr_1 | g10490 | g10490.t1 | cds | g10490.t1.CDS3 | 10154034 | 10154687 |
| chr_1 | g10490 | g10490.t1 | exon | g10490.t1.exon4 | 10154765 | 10154901 |
| chr_1 | g10490 | g10490.t1 | cds | g10490.t1.CDS4 | 10154765 | 10154901 |
| chr_1 | g10490 | g10490.t1 | TTS | g10490.t1 | 10154986 | 10154986 |
Sequences
>g10490.t1 Gene=g10490 Length=1788
ATGTATACACCTAATTTTCTCATATTGCTGTTATTATTTCAACAGTCGATTCGTTTAGAT
GCCATTGATACGCGATATGTATGGGGTTATCAATGCCTCGAAAATAAATGCGTAAAAACA
AAAATAACGTCTGAAAATCAGAATTCAATTTTAGCTTTACAAGTCTGTCGTCTTTATTGC
TATGAAGACATTGGAACATTATGGCCTATTCCAACTGGAAATATTGTCAATTCGAAAACT
ATAGTTCAATTCAATTCACAGTCAATAAATGTCGAATCAGAATTGTTATTTGATAATAAA
TATTTTATTGAAGCTGTCAATCGATTTAAAGATATGCAATTGAAGAAAATTCCTTCTAAG
TATCCTTTGATCTCAGGTGGAAAACGATTAAATGTGACGATCAATGTTGAAACAAATGAT
ACAATCTTAAACTATGACACAAATGAAAGCTATCAATTGACATTAATTGAAGAATCAAAT
CAACTTAGAGTGACTATCACTGCTAAAAATTTTTTTGGTGCTAGACATGCTCTCGAAACA
CTTTCACAATTGATTGTTCATGATGAACTCAATAGGCAACTCGTTACTCTATCATCGGTA
TCAATAAGTGATGAACCAAAATTCAAGCATCGTGGCATTTCTATGGACACTTCGAGAACA
TTCTTTTCTGTTGATGTGATAAAACGAACAATCAACGGTTTAGCAATGGCAAAACTTAAC
AATTTTCATTGGCACATCACTGATGCTCAAAGTTTCCCTATGGAAATGAAAAAACATTCA
GATCTCACAAAATATGGCGCATATTCGCATAAAAAAATATACAAAGTTGAAGATATTCAA
GATATTGTTGAATATGCAAAAGCACGAGGTGTTAAAGTTATTCCAGAATTTGATTTGCCT
GCTCATATTGGTGAAGGATGGCAGAAAAAGAAAGAACATCTTACAACCTGTCTCAATTAC
AAGCCTTATCTTAAATATTGTTGGCAACCACCATGCGGTCATGTAAATCCAACTGTAGAT
GAAGTCTATGATGTACTTGAAGATATTTATAAAGAAATGATTGACATATTCAATCCACCG
GCTTTTCACACTGGTGGTGATGAGATCTTCTTCTCTTGTTGGAATTCCAGCGAGAGTTTG
AGACAGTGGATGACAGATAAAGGTTGGACATTAGATGAAGAACATTTTATGAAATTGTGG
GGTCAATTTCAGACAAGAGCACAAGAAAGACTTGACAAGGTTAGTAATGGAAATCTTCCG
TTGATCATTTGGACGAGTGAATTGACTGAAGAACCTTATGTAAGTGAATATTTGGATAAG
AGTAGATATATAATACAAGTTTGGACAGATTATAAAGATGTAAGAATTCAAAGACTCTTA
GAAAGTGGCTATCGATTGATCATTTCCAATTATGATGCGCTCTATCTTGACTGTGGTTAT
GGTTCATGGGCATCTGATGGCTATATTTGGTGCTCACCATATCATGAGTGGCAAAAAATC
TATCAAAATTCTCTCGATCAATTGGGCGGCGAGCGCGTCAATCAAATATTTGGTGCAGAA
GCAACAATTTGGTCTGAATCGATTGATGAACGAAACATTGATGCAAGAATATGGCCTCGA
GCTTCAGCATTAGCAGAGCGACTCTGGTCGAATCCGTCAGAACATTGGAGGCGAGCAGAA
CCAAGGCTATTGATGCACACAGATTATTTGAAAATTGTCGGGGATTTAACTGCTGAACCC
CTGCAGCATGAGTGGTGTATTCATAATGAAGGATTATGCCAATTATAA
>g10490.t1 Gene=g10490 Length=595
MYTPNFLILLLLFQQSIRLDAIDTRYVWGYQCLENKCVKTKITSENQNSILALQVCRLYC
YEDIGTLWPIPTGNIVNSKTIVQFNSQSINVESELLFDNKYFIEAVNRFKDMQLKKIPSK
YPLISGGKRLNVTINVETNDTILNYDTNESYQLTLIEESNQLRVTITAKNFFGARHALET
LSQLIVHDELNRQLVTLSSVSISDEPKFKHRGISMDTSRTFFSVDVIKRTINGLAMAKLN
NFHWHITDAQSFPMEMKKHSDLTKYGAYSHKKIYKVEDIQDIVEYAKARGVKVIPEFDLP
AHIGEGWQKKKEHLTTCLNYKPYLKYCWQPPCGHVNPTVDEVYDVLEDIYKEMIDIFNPP
AFHTGGDEIFFSCWNSSESLRQWMTDKGWTLDEEHFMKLWGQFQTRAQERLDKVSNGNLP
LIIWTSELTEEPYVSEYLDKSRYIIQVWTDYKDVRIQRLLESGYRLIISNYDALYLDCGY
GSWASDGYIWCSPYHEWQKIYQNSLDQLGGERVNQIFGAEATIWSESIDERNIDARIWPR
ASALAERLWSNPSEHWRRAEPRLLMHTDYLKIVGDLTAEPLQHEWCIHNEGLCQL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 24 | g10490.t1 | CDD | cd06562 | GH20_HexA_HexB-like | 208 | 574 | 9.82079E-152 |
| 16 | g10490.t1 | Gene3D | G3DSA:3.30.379.10 | Chitobiase | 24 | 205 | 2.2E-47 |
| 17 | g10490.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 206 | 595 | 6.4E-138 |
| 3 | g10490.t1 | PANTHER | PTHR22600 | BETA-HEXOSAMINIDASE | 7 | 593 | 6.7E-152 |
| 4 | g10490.t1 | PANTHER | PTHR22600:SF42 | BETA-HEXOSAMINIDASE | 7 | 593 | 6.7E-152 |
| 23 | g10490.t1 | PIRSF | PIRSF001093 | B-hxosamndse_ab_euk_ | 26 | 595 | 3.3E-154 |
| 11 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 164 | 184 | 6.2E-54 |
| 5 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 202 | 219 | 6.2E-54 |
| 7 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 231 | 252 | 6.2E-54 |
| 12 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 285 | 302 | 6.2E-54 |
| 8 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 335 | 353 | 6.2E-54 |
| 6 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 357 | 370 | 6.2E-54 |
| 10 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 516 | 532 | 6.2E-54 |
| 9 | g10490.t1 | PRINTS | PR00738 | Glycosyl hydrolase family 20 signature | 533 | 550 | 6.2E-54 |
| 1 | g10490.t1 | Pfam | PF14845 | beta-acetyl hexosaminidase like | 128 | 184 | 3.4E-11 |
| 2 | g10490.t1 | Pfam | PF00728 | Glycosyl hydrolase family 20, catalytic domain | 208 | 551 | 4.6E-93 |
| 19 | g10490.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 20 | g10490.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 21 | g10490.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 13 | - |
| 22 | g10490.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 18 | g10490.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 595 | - |
| 13 | g10490.t1 | SUPERFAMILY | SSF55545 | beta-N-acetylhexosaminidase-like domain | 67 | 207 | 1.79E-21 |
| 14 | g10490.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 208 | 563 | 2.92E-103 |
| 15 | g10490.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004563 | beta-N-acetylhexosaminidase activity | MF |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K12373 (hexosaminidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed