Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Heparan-alpha-glucosaminide N-acetyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10496 g10496.t4 TTS g10496.t4 10204496 10204496
chr_1 g10496 g10496.t4 isoform g10496.t4 10204623 10205638
chr_1 g10496 g10496.t4 exon g10496.t4.exon1 10204623 10205564
chr_1 g10496 g10496.t4 cds g10496.t4.CDS1 10204623 10205489
chr_1 g10496 g10496.t4 exon g10496.t4.exon2 10205623 10205638
chr_1 g10496 g10496.t4 TSS g10496.t4 10206652 10206652

Sequences

>g10496.t4 Gene=g10496 Length=958
AAAGAAAGTTATCAAGCGGTCAATCACATTATTTCTCCTTGGTTTATGTCTCAATACGCT
ATATGGTGCGTCTTTTCAAAGCATTCGTATAATGGGAGTTTTGCAACGATTTGGAATCGC
TTATTTGGTTATTTCACTTATTCATCTTGCTTTTGTCAGCAATTCAATCAATGCAGAAAC
AAGAATCCAACAGTTATTTTATGATGTTCGAATGATATGGAAACAATGGTTGATTGTTCT
TAGTTTTGTAATCTTCCACTTGATTATGATTTTTGGAATAAAAGCATCAAATTGTGAGCG
AGGATATTTTGGTCCAGGTGGTATTCATGAATACATGGCACATGAAAATTGCACGGGTGG
CATCGCTGGATTTGTTGATCGAAATGTTTTGGGTGAGAGTCACATGTATCAACGTGCGAA
AATTCGATCAGTTTATGATGCTCAAGTATTTGATCCTGAAGGAGTTTTTGGATGCTTGCT
GACATGTTTGCAAGTATTTTTGGGTCTCCAGTGTGGAGTTACTTTATTGGTTTACCCAAC
AGCAAAAGAAAGATTAAGACGATGGATAATTTGGGGAATTGGATTGCTGATTCTTACATG
TTTTTTAACTTTTTTCTCAGTTGAAAATGGTCTCATACCAATTAATAAGAATTTATGGTC
ATTATCATTTGTTACCATCACTGCAGGCTTAGCATTTCTTCTTCTTAGTTTAATTTATTT
TTTGGTTGATGTTAAAAACATTGGTGACGATTTTTGGACAATTTTCTTATATCCTGGAAT
GAATGCGATTGTTCTTTATGTAGGTCACTCTGTCTTTCACAGCATGTGGCCTTTTCATTG
GTCAGTTGATAATATGAACACTCATTTTCTTTTGCTACTAGAAAATTTATATACTGTCAT
GATTTGGGTACTCATTTCCCACTATTTGTATCACAAAGAACTGTTTTATAATATTTAA

>g10496.t4 Gene=g10496 Length=288
MGVLQRFGIAYLVISLIHLAFVSNSINAETRIQQLFYDVRMIWKQWLIVLSFVIFHLIMI
FGIKASNCERGYFGPGGIHEYMAHENCTGGIAGFVDRNVLGESHMYQRAKIRSVYDAQVF
DPEGVFGCLLTCLQVFLGLQCGVTLLVYPTAKERLRRWIIWGIGLLILTCFLTFFSVENG
LIPINKNLWSLSFVTITAGLAFLLLSLIYFLVDVKNIGDDFWTIFLYPGMNAIVLYVGHS
VFHSMWPFHWSVDNMNTHFLLLLENLYTVMIWVLISHYLYHKELFYNI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
1 g10496.t4 PANTHER PTHR31061 LD22376P 2 285 1.1E-70
2 g10496.t4 PANTHER PTHR31061:SF19 HEPARAN-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE 2 285 1.1E-70
14 g10496.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
21 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 26 -
11 g10496.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 27 45 -
24 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 46 63 -
17 g10496.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 64 123 -
20 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 124 146 -
12 g10496.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 147 157 -
18 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 158 177 -
16 g10496.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 178 188 -
23 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 189 212 -
13 g10496.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 213 223 -
22 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 224 246 -
15 g10496.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 247 257 -
19 g10496.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 258 280 -
10 g10496.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 281 288 -
5 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 4 26 -
9 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 46 63 -
7 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 124 146 -
3 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 158 177 -
8 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 192 214 -
4 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 221 243 -
6 g10496.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 258 280 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005765 lysosomal membrane CC
GO:0015019 heparan-alpha-glucosaminide N-acetyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values