Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Nucleoporin Nup35.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10553 g10553.t1 isoform g10553.t1 10450606 10451874
chr_1 g10553 g10553.t1 exon g10553.t1.exon1 10450606 10450720
chr_1 g10553 g10553.t1 cds g10553.t1.CDS1 10450606 10450720
chr_1 g10553 g10553.t1 exon g10553.t1.exon2 10450839 10451718
chr_1 g10553 g10553.t1 cds g10553.t1.CDS2 10450839 10451718
chr_1 g10553 g10553.t1 exon g10553.t1.exon3 10451778 10451874
chr_1 g10553 g10553.t1 cds g10553.t1.CDS3 10451778 10451874
chr_1 g10553 g10553.t1 TSS g10553.t1 10451964 10451964
chr_1 g10553 g10553.t1 TTS g10553.t1 NA NA

Sequences

>g10553.t1 Gene=g10553 Length=1092
ATGGAGAAAATGCAGCTTGGAAGCCCTCAAAATACCTATTTACCTAATTTTCTAATGGGA
GAACAAACGCCGACTAATCAACATCATCGTTCTTTTTCAAATTCACCAACTGCATCACCT
TCTAGCATGCAACAAAAGTTTTTGACAAGTTTACAAAGTCCCAATCCTATTTTTAATTCA
TCAATGAACCAATCATTTAACAATATTCATCATCATCAACAACAAATTTTTCAAACACAA
AATCCAGCAAACAATTCAGCAGCAAATAACAATAATAATAGTATTTCTGGACCACCCATC
AATTCACTCTTTGATAATTTAAGAAACGAAATGAATTTTCAAACTCCCACAAAAAGCTTC
TATCATCAATCAATAATTCATCAGGATACTCCGCAACCTATGCATAATAATTCTGAATTT
AATCAATCACGTATATTATCACCTATTCCACTTTCTTTCCCATCTGGGGCTGTTAATAAT
GATTTTAATACTTCCACTTCCTTTCAAAACCTTATGCAGCAATCTGGTGCAATTTACAAC
AATATAAATCAATCAATGAATGGCTATAGTCAAAAGGATTTTTGGGTAACAGTTTTTGGC
TTTCCGTCTGAAGCTCTAACAATCGTTCTATCACATTTTTCTGGTTGTGGAACAATTCTT
GAGAAAGTCTGTTCATCGGGCAATTGGGTTCATTTAAGATATTCTTCTCGTGCCGAATGC
GATAAGTCTCTTCTTTATAATGGAAAAATTATTGGAAATAATTTAATGATTGGTGTTGTT
CGATGTGAAGACGAATCAATTACAGAAAAAGAAAACATGAATTATAATCCTCAAACTTCC
ATCACTAAAATTCGTTCATTGACTCAAGCTGCATACAAGTCGGTGCGTGATGAGACGGAA
GTTGTACCAAGTGGTATCGAAGAGCCAAAAAAGACAACAGGCATAGTTAATAAAGCGATG
GATCTTATTTTTGGATGTTGCATACGCTCTTTTCAATTCGGGACAACACTTGGAATGGAA
TTGAATGGAAAAAAAATTATTTCTATTCAGTCTTTCATTGAGAGAGGATCATCTTTTGAA
TCGAAATTATAA

>g10553.t1 Gene=g10553 Length=363
MEKMQLGSPQNTYLPNFLMGEQTPTNQHHRSFSNSPTASPSSMQQKFLTSLQSPNPIFNS
SMNQSFNNIHHHQQQIFQTQNPANNSAANNNNNSISGPPINSLFDNLRNEMNFQTPTKSF
YHQSIIHQDTPQPMHNNSEFNQSRILSPIPLSFPSGAVNNDFNTSTSFQNLMQQSGAIYN
NINQSMNGYSQKDFWVTVFGFPSEALTIVLSHFSGCGTILEKVCSSGNWVHLRYSSRAEC
DKSLLYNGKIIGNNLMIGVVRCEDESITEKENMNYNPQTSITKIRSLTQAAYKSVRDETE
VVPSGIEEPKKTTGIVNKAMDLIFGCCIRSFQFGTTLGMELNGKKIISIQSFIERGSSFE
SKL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g10553.t1 CDD cd12441 RRM_Nup53_like 193 264 4.07772E-29
5 g10553.t1 Gene3D G3DSA:3.30.70.330 - 190 262 4.2E-23
8 g10553.t1 MobiDBLite mobidb-lite consensus disorder prediction 24 47 -
2 g10553.t1 PANTHER PTHR21527 NUCLEOPORIN NUP35 4 325 4.8E-34
3 g10553.t1 PANTHER PTHR21527:SF6 NUCLEOPORIN NUP35 4 325 4.8E-34
6 g10553.t1 PIRSF PIRSF038119 NUP53 1 326 4.2E-55
1 g10553.t1 Pfam PF05172 Nup53/35/40-type RNA recognition motif 191 266 6.7E-20
9 g10553.t1 ProSiteProfiles PS51472 RNA-recognition motif (RRM) Nup35-type domain profile. 190 269 29.289
4 g10553.t1 SUPERFAMILY SSF54928 RNA-binding domain, RBD 192 267 5.45E-12

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0031965 nuclear membrane CC
GO:0006913 nucleocytoplasmic transport BP
GO:0017056 structural constituent of nuclear pore MF
GO:0003676 nucleic acid binding MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values