General information for g10774.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g10774 is located on chr_1: 11895168..11897162 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10774 | g10774.t1 | isoform | g10774.t1 | 11895168 | 11897162 |
| chr_1 | g10774 | g10774.t1 | exon | g10774.t1.exon1 | 11895168 | 11896155 |
| chr_1 | g10774 | g10774.t1 | cds | g10774.t1.CDS1 | 11895168 | 11896155 |
| chr_1 | g10774 | g10774.t1 | exon | g10774.t1.exon2 | 11896210 | 11897162 |
| chr_1 | g10774 | g10774.t1 | cds | g10774.t1.CDS2 | 11896210 | 11897162 |
| chr_1 | g10774 | g10774.t1 | TSS | g10774.t1 | NA | NA |
| chr_1 | g10774 | g10774.t1 | TTS | g10774.t1 | NA | NA |
Sequences
>g10774.t1 Gene=g10774 Length=1941
ATGACTTTTTTACTTATAAATCTAATAATTTTTGGATCTTCATTTGCGCTGGACGCAAAT
AAATTTATTAGAGATACAACAAAACAGGTTAATTCAGATATGATTAAAGTAATTCATTTT
GTCTTCAAAGAAATTTATTTTAATCAATACCGAACTGTAAACGTAATGGATTGTGTTGAG
AATCCTAATGACCAATATTTTAAAGATTTTAAAAATGCTGTTTTTTTGATTGACAATTTG
ACTATACCATATCAATTTCGAAAAGCCAACAATTCATATATGGAACCAGGAAAAACTCGT
TTAAAAGTAAAGAATTTATTCATTATAGATACATACAACTCATATTTAAACATTGAACGA
TTCTTTTTGATTGATCGATTTAACTTTCGAGGTCAATATCTTTTTGCTTTCATTCATGGT
CGACTTGATGATGAAGCTACAGAGAAAATATTTAAAAGAATGTGGGAAAAAGGACTTTAC
AATGTAAATTTTTTGTATGAAGAAAATAATGTAGTAAAAATGACTACTTTTCAACCTTTT
CAAACAAATACTTGTGAGAGCATGGAAGAAATATTAATCGACACATTTGTGAATGGAACA
TTCATAAGAGGAGGAAATATTGAAATAATTTTCCCTAAAAATAAATTTAAAAATCTTTAC
AAATGTCCTATAAAAATTGTAACTTTTGAAAGATGTCCAGCAATGTGCTACAACAAGAAA
GAAAATAAATTTTCAGGATATGACTGGGAATTAATTCAAGAGATTTCCCAACAATTAAAT
TTTAAAATGGAAGTCAAATATTTAGAAGGAGAAGAGCAATGGGGAACAATTTTAAGCAAT
GGCTCGCTTACTGGAGGATTAAAAGAACTTGTTGAGAAGCGTGCTGAAATTGGTATTGGA
AATTATTTCTTGAGGGAAAGTCGAACAAGAATTGTTGATTATAGTGCTGCATATTTTAGC
ATTTCGATCGTGTTAGTAATTCCACCAGCCACAAAACTGTCACCATTTGAGAAACTTCTT
CGACCATTTGAAATAATTATTTGGATTTTATTATTCATCACATTTAGCATTGGTCTTCTT
GTCATTTTTATTATCAACTACAAATTAAAAAATTTTAAAAGTTTTGTATTTGGAAGAAAA
GTGAACACACCAATATTAAACATGTTTATTGCAATATTCGGTCAACAGCAAACAATTTTG
CCAGGAAGAAATTATGCGAGATTTATTTTAATGATGTTTCTTATTTTTTGTCTTGTTAAT
CGAAATGTTTATCAAGGTGCACTTTATATTTTCTTACAATCTGATGGACGTCATAAAGAC
ATTAAGAGTATTGATGAAATGATTGAAAAAGATTTTAAATTTTACGTTTATGAATCTTAT
ATTGATTTTGTTCATAATTCATCAGAAATTTATAAGAGAAAAATTATTATCAGAAATGAA
TCAGAATTGCCATTTTCCAAAGAAGTTGATGAAAATTTAAAAGCAGCTTTTATGACACCA
CAGACAAATGTTATTAACCGAAATCAAATGAATCAAGGAAAATTTTCACTAAAAGTTTGT
GATGAGCTGGTCTCAGTCATTAATATTGCAATATTTTTTAAAAGAAATTTCTTTCTAAAA
ACTGCAATTGATGAAAATTTAAAAAACTTTGATGCAGCAGGTTTTTTTACTTACTGGTTC
AATAAACATGCAGATAAGAAATTTTTGAATGCCAAAGATGGAATAACCGGTCCTAAAAAG
TTAAATATGGAACATTTATTTGGTGTTTTTAATATCATGCTGATTGGGTATGCAATAGCA
ACAATTTTTTTCATTCTTGAAATCTTTATTTTTAAGTTCAAAGGATTTCGAATGAATAAA
TATAAGAGCAGACTCATTTTCAATGTTTCAAATATAAATGAAGAAGATACAAATGAGAAA
ATGCATGAAATAATTACATAA
>g10774.t1 Gene=g10774 Length=646
MTFLLINLIIFGSSFALDANKFIRDTTKQVNSDMIKVIHFVFKEIYFNQYRTVNVMDCVE
NPNDQYFKDFKNAVFLIDNLTIPYQFRKANNSYMEPGKTRLKVKNLFIIDTYNSYLNIER
FFLIDRFNFRGQYLFAFIHGRLDDEATEKIFKRMWEKGLYNVNFLYEENNVVKMTTFQPF
QTNTCESMEEILIDTFVNGTFIRGGNIEIIFPKNKFKNLYKCPIKIVTFERCPAMCYNKK
ENKFSGYDWELIQEISQQLNFKMEVKYLEGEEQWGTILSNGSLTGGLKELVEKRAEIGIG
NYFLRESRTRIVDYSAAYFSISIVLVIPPATKLSPFEKLLRPFEIIIWILLFITFSIGLL
VIFIINYKLKNFKSFVFGRKVNTPILNMFIAIFGQQQTILPGRNYARFILMMFLIFCLVN
RNVYQGALYIFLQSDGRHKDIKSIDEMIEKDFKFYVYESYIDFVHNSSEIYKRKIIIRNE
SELPFSKEVDENLKAAFMTPQTNVINRNQMNQGKFSLKVCDELVSVINIAIFFKRNFFLK
TAIDENLKNFDAAGFFTYWFNKHADKKFLNAKDGITGPKKLNMEHLFGVFNIMLIGYAIA
TIFFILEIFIFKFKGFRMNKYKSRLIFNVSNINEEDTNEKMHEIIT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10774.t1 | Gene3D | G3DSA:3.40.190.10 | - | 242 | 579 | 9.9E-43 |
| 9 | g10774.t1 | Gene3D | G3DSA:3.40.190.10 | - | 322 | 529 | 9.9E-43 |
| 7 | g10774.t1 | Gene3D | G3DSA:1.10.287.70 | - | 332 | 607 | 9.9E-43 |
| 3 | g10774.t1 | PANTHER | PTHR42643:SF16 | IONOTROPIC RECEPTOR 11A-RELATED | 51 | 615 | 4.0E-60 |
| 4 | g10774.t1 | PANTHER | PTHR42643 | IONOTROPIC RECEPTOR 20A-RELATED | 51 | 615 | 4.0E-60 |
| 2 | g10774.t1 | Pfam | PF10613 | Ligated ion channel L-glutamate- and glycine-binding site | 238 | 327 | 3.5E-12 |
| 1 | g10774.t1 | Pfam | PF00060 | Ligand-gated ion channel | 347 | 596 | 2.0E-7 |
| 16 | g10774.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 17 | g10774.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 18 | g10774.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 24 | g10774.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 14 | g10774.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 313 | - |
| 22 | g10774.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 314 | 333 | - |
| 12 | g10774.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 334 | 344 | - |
| 21 | g10774.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 345 | 365 | - |
| 13 | g10774.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 366 | 407 | - |
| 19 | g10774.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 408 | 432 | - |
| 11 | g10774.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 433 | 514 | - |
| 20 | g10774.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 515 | 533 | - |
| 15 | g10774.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 534 | 585 | - |
| 23 | g10774.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 586 | 611 | - |
| 10 | g10774.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 612 | 646 | - |
| 28 | g10774.t1 | SMART | SM00918 | Lig_chan_Glu_bd_2 | 233 | 292 | 3.6E-4 |
| 5 | g10774.t1 | SUPERFAMILY | SSF53850 | Periplasmic binding protein-like II | 223 | 566 | 2.28E-24 |
| 6 | g10774.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 27 | g10774.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 345 | 367 | - |
| 25 | g10774.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 405 | 424 | - |
| 26 | g10774.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 589 | 611 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0015276 | ligand-gated ion channel activity | MF |
| GO:0004970 | ionotropic glutamate receptor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed