Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t10 TSS g10883.t10 12437137 12437137
chr_1 g10883 g10883.t10 isoform g10883.t10 12437248 12439672
chr_1 g10883 g10883.t10 exon g10883.t10.exon1 12437248 12437337
chr_1 g10883 g10883.t10 cds g10883.t10.CDS1 12437248 12437337
chr_1 g10883 g10883.t10 exon g10883.t10.exon2 12437431 12437468
chr_1 g10883 g10883.t10 cds g10883.t10.CDS2 12437431 12437468
chr_1 g10883 g10883.t10 exon g10883.t10.exon3 12437637 12438215
chr_1 g10883 g10883.t10 cds g10883.t10.CDS3 12437637 12438215
chr_1 g10883 g10883.t10 exon g10883.t10.exon4 12438279 12439672
chr_1 g10883 g10883.t10 cds g10883.t10.CDS4 12438279 12439551
chr_1 g10883 g10883.t10 TTS g10883.t10 12439790 12439790

Sequences

>g10883.t10 Gene=g10883 Length=2101
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATAATTTCAGTCACTTTAACCGATTGCAATGT
AGATATAAATCCGAAGGTGTAAAAGGAGCAGTCATTGGTATTGACTTAGGAACAACAAAT
TCATGTGTGGCTGTTATGGAAGGAAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCA
AGAACAACACCATCTCATGTTGCATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCA
GCAAAGAGACAAGCTGTGACAAATTCTGCAAATACCTTTTATGCTACAAAACGTTTGATT
GGACGCCGATTTGAAGATCCCGAGATTAAGAAAGATATGAAGAACTTGTCATATAAAGTA
GTCAAAGCTTCAAATGGTGATGCATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCT
TCACAAATTGGTGCTTTCGTTTTAATGAAAATGAAGGAAACTGCAGAAGCATATCTTAAT
CAACCAGTTAAGAATGCAGTTGTTACTGTTCCTGCATATTTTAATGATTCACAACGTCAA
GCAACAAAAGATGCTGGTCAAATTTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCA
ACAGCTGCTGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTAT
GATCTTGGCGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAA
GTAAAATCAACAAATGGTGATACAATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTC
AATTTCTTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATG
GCAATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTT
CAAACTGACATCAATTTGCCATATCTTACAATGGACGCAAGTGGACCTAAACACATGAAC
CTCAAGATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTCATTAAACGTACAATT
GCACCTTGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTT
TTGTTAGTCGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTC
GGTAGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTA
CAAGGTGGTGTTTTGGCTGGAGATGTAACTGATGTACTTTTACTTGATGTTACACCATTG
TCACTTGGTATTGAAACACTTGGCGGCGTCTTTACACGTTTGATCACAAGAAACACAACA
ATTCCAACAAAGAAATCACAAGTATTCTCAACAGCAGCTGATGGACAAACACAAGTCGAA
ATCAAAGTTCACCAGGGTGAACGTGAAATGGCATCTGATAACAAATTACTTGGAGCTTTT
ACACTTGTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAAATCGAAGTTACATTTGAT
ATTGATGCAAACGGTATTGTACATGTATCAGCTCGTGACAAGGGAACTGGCAAGGAACAA
CAAATTGTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAAATCGAAAACATGATAAAG
AATGCCGAACAATATGCACAAGCTGATAAGACAAAGAAGGATCGAATTGAGGCTATCAAT
CAGGCTGAGTCAATTGTGCACGATACAGAAACAAAAATGGAAGAATTCAAAGATCAATTA
CCAAAGGAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAAGTACGAACACTCCTTGCC
GATAAAGAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACTGGAGCTCTACAACAATCA
TCACTGAAACTCTTCGAAATGGCATACAAAAAAGTGAGTAAAATGAATCATAATTCATAA
TGAGTAAAGTTACTAATTAGCTTTTGTATCTCTTTTATTGCAGATGGCATCAGAATGTGA
GAGCTCAGGAAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAGGAAGATAAAAATTA
A

>g10883.t10 Gene=g10883 Length=659
MLSATKHLLKNTNITQTLMNTRNLSSLMKNNFSHFNRLQCRYKSEGVKGAVIGIDLGTTN
SCVAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLI
GRRFEDPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLN
QPVKNAVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVY
DLGGGTFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAM
AMQRLKEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTI
APCQKALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAV
QGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAADGQTQVE
IKVHQGEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDKGTGKEQ
QIVIQSSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKMEEFKDQL
PKEECDKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKVSKMNHNS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
26 g10883.t10 CDD cd11733 HSPA9-like_NBD 48 425 0.0
23 g10883.t10 Coils Coil Coil 302 322 -
24 g10883.t10 Coils Coil Coil 579 599 -
25 g10883.t10 Coils Coil Coil 605 625 -
19 g10883.t10 Gene3D G3DSA:3.30.420.40 - 52 420 8.0E-161
21 g10883.t10 Gene3D G3DSA:3.30.30.30 - 114 166 8.0E-161
20 g10883.t10 Gene3D G3DSA:3.30.420.40 - 232 405 8.0E-161
18 g10883.t10 Gene3D G3DSA:3.90.640.10 Actin; Chain A 271 358 8.0E-161
17 g10883.t10 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 421 549 1.7E-59
22 g10883.t10 Gene3D G3DSA:1.20.1270.10 - 550 653 1.9E-26
4 g10883.t10 Hamap MF_00332 Chaperone protein DnaK [dnaK]. 48 659 34.717762
2 g10883.t10 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 38 630 9.3E-286
3 g10883.t10 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 38 630 9.3E-286
6 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 50 63 3.2E-80
10 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 78 90 3.2E-80
12 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 101 109 3.2E-80
8 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 186 206 3.2E-80
13 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 245 255 3.2E-80
11 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 377 393 3.2E-80
7 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 408 428 3.2E-80
5 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 431 450 3.2E-80
9 g10883.t10 PRINTS PR00301 70kDa heat shock protein signature 512 528 3.2E-80
1 g10883.t10 Pfam PF00012 Hsp70 protein 51 649 1.6E-270
27 g10883.t10 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 54 61 -
29 g10883.t10 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 239 252 -
28 g10883.t10 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 380 394 -
15 g10883.t10 SUPERFAMILY SSF53067 Actin-like ATPase domain 51 232 2.16E-63
16 g10883.t10 SUPERFAMILY SSF53067 Actin-like ATPase domain 234 425 5.19E-66
14 g10883.t10 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 427 583 8.89E-63
30 g10883.t10 TIGRFAM TIGR02350 prok_dnaK: chaperone protein DnaK 51 649 5.4E-282

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values