Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t11 TSS g10883.t11 12437137 12437137
chr_1 g10883 g10883.t11 isoform g10883.t11 12437248 12439672
chr_1 g10883 g10883.t11 exon g10883.t11.exon1 12437248 12437337
chr_1 g10883 g10883.t11 cds g10883.t11.CDS1 12437248 12437337
chr_1 g10883 g10883.t11 exon g10883.t11.exon2 12437605 12438215
chr_1 g10883 g10883.t11 cds g10883.t11.CDS2 12437605 12438215
chr_1 g10883 g10883.t11 exon g10883.t11.exon3 12438279 12439524
chr_1 g10883 g10883.t11 cds g10883.t11.CDS3 12438279 12439524
chr_1 g10883 g10883.t11 exon g10883.t11.exon4 12439605 12439672
chr_1 g10883 g10883.t11 cds g10883.t11.CDS4 12439605 12439670
chr_1 g10883 g10883.t11 TTS g10883.t11 12439790 12439790

Sequences

>g10883.t11 Gene=g10883 Length=2015
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATTTTCTCATAAAAACATTCTCATTTGTCAAC
AGATCCGAAGGTGTAAAAGGAGCAGTCATTGGTATTGACTTAGGAACAACAAATTCATGT
GTGGCTGTTATGGAAGGAAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCAAGAACA
ACACCATCTCATGTTGCATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCAGCAAAG
AGACAAGCTGTGACAAATTCTGCAAATACCTTTTATGCTACAAAACGTTTGATTGGACGC
CGATTTGAAGATCCCGAGATTAAGAAAGATATGAAGAACTTGTCATATAAAGTAGTCAAA
GCTTCAAATGGTGATGCATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCTTCACAA
ATTGGTGCTTTCGTTTTAATGAAAATGAAGGAAACTGCAGAAGCATATCTTAATCAACCA
GTTAAGAATGCAGTTGTTACTGTTCCTGCATATTTTAATGATTCACAACGTCAAGCAACA
AAAGATGCTGGTCAAATTTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCAACAGCT
GCTGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTATGATCTT
GGCGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAAGTAAAA
TCAACAAATGGTGATACAATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTCAATTTC
TTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATGGCAATG
CAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTTCAAACT
GACATCAATTTGCCATATCTTACAATGGACGCAAGTGGACCTAAACACATGAACCTCAAG
ATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTCATTAAACGTACAATTGCACCT
TGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTTTTGTTA
GTCGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTCGGTAGA
CAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTACAAGGT
GGTGTTTTGGCTGGAGATGTAACTGATGTACTTTTACTTGATGTTACACCATTGTCACTT
GGTATTGAAACACTTGGCGGCGTCTTTACACGTTTGATCACAAGAAACACAACAATTCCA
ACAAAGAAATCACAAGTATTCTCAACAGCAGCTGATGGACAAACACAAGTCGAAATCAAA
GTTCACCAGGGTGAACGTGAAATGGCATCTGATAACAAATTACTTGGAGCTTTTACACTT
GTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAAATCGAAGTTACATTTGATATTGAT
GCAAACGGTATTGTACATGTATCAGCTCGTGACAAGGGAACTGGCAAGGAACAACAAATT
GTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAAATCGAAAACATGATAAAGAATGCC
GAACAATATGCACAAGCTGATAAGACAAAGAAGGATCGAATTGAGGCTATCAATCAGGCT
GAGTCAATTGTGCACGATACAGAAACAAAAATGGAAGAATTCAAAGATCAATTACCAAAG
GAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAAGTACGAACACTCCTTGCCGATAAA
GAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACTGGAGCTCTACAACAATCATCACTG
AAACTCTTCGAAATGGCATACAAAAAAAATGTGAGAGCTCAGGAAGCAAATCAAGTAGTG
AAGAGGGTGGTGAAAAGAAGGAAGATAAAAATTAA

>g10883.t11 Gene=g10883 Length=671
MLSATKHLLKNTNITQTLMNTRNLSSLMKNFLIKTFSFVNRSEGVKGAVIGIDLGTTNSC
VAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLIGR
RFEDPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLNQP
VKNAVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVYDL
GGGTFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAMAM
QRLKEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTIAP
CQKALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAVQG
GVLAGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAADGQTQVEIK
VHQGEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDKGTGKEQQI
VIQSSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKMEEFKDQLPK
EECDKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKNVRAQEANQVV
KRVVKRRKIKI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
28 g10883.t11 CDD cd11733 HSPA9-like_NBD 46 423 0.0
27 g10883.t11 Coils Coil Coil 300 320 -
25 g10883.t11 Coils Coil Coil 577 597 -
24 g10883.t11 Coils Coil Coil 603 623 -
26 g10883.t11 Coils Coil Coil 671 671 -
21 g10883.t11 Gene3D G3DSA:3.30.420.40 - 50 418 8.4E-161
22 g10883.t11 Gene3D G3DSA:3.30.30.30 - 112 164 8.4E-161
20 g10883.t11 Gene3D G3DSA:3.30.420.40 - 230 403 8.4E-161
19 g10883.t11 Gene3D G3DSA:3.90.640.10 Actin; Chain A 269 356 8.4E-161
18 g10883.t11 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 419 547 1.8E-59
23 g10883.t11 Gene3D G3DSA:1.20.1270.10 - 548 651 1.7E-26
4 g10883.t11 Hamap MF_00332 Chaperone protein DnaK [dnaK]. 46 663 34.851917
2 g10883.t11 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 24 632 3.8E-285
3 g10883.t11 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 24 632 3.8E-285
8 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 48 61 3.7E-80
12 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 76 88 3.7E-80
6 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 99 107 3.7E-80
11 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 184 204 3.7E-80
5 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 243 253 3.7E-80
9 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 375 391 3.7E-80
7 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 406 426 3.7E-80
13 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 429 448 3.7E-80
10 g10883.t11 PRINTS PR00301 70kDa heat shock protein signature 510 526 3.7E-80
1 g10883.t11 Pfam PF00012 Hsp70 protein 49 647 1.7E-270
29 g10883.t11 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 52 59 -
31 g10883.t11 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 237 250 -
30 g10883.t11 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 378 392 -
17 g10883.t11 SUPERFAMILY SSF53067 Actin-like ATPase domain 49 230 2.16E-63
16 g10883.t11 SUPERFAMILY SSF53067 Actin-like ATPase domain 232 423 5.37E-66
15 g10883.t11 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 425 581 9.16E-63
14 g10883.t11 SUPERFAMILY SSF100934 Heat shock protein 70kD (HSP70), C-terminal subdomain 548 653 7.19E-12
32 g10883.t11 TIGRFAM TIGR02350 prok_dnaK: chaperone protein DnaK 49 647 5.8E-282

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values