Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t18 isoform g10883.t18 12438337 12439672
chr_1 g10883 g10883.t18 exon g10883.t18.exon1 12438337 12439524
chr_1 g10883 g10883.t18 cds g10883.t18.CDS1 12438376 12439524
chr_1 g10883 g10883.t18 exon g10883.t18.exon2 12439595 12439672
chr_1 g10883 g10883.t18 cds g10883.t18.CDS2 12439595 12439672
chr_1 g10883 g10883.t18 TTS g10883.t18 12439790 12439790
chr_1 g10883 g10883.t18 TSS g10883.t18 NA NA

Sequences

>g10883.t18 Gene=g10883 Length=1266
AAAGGTGTCTTTGAAGTAAAATCAACAAATGGTGATACAATGTTGGGTGGTGAGGATTTC
GACAACGCAATTGTCAATTTCTTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATT
AGAAAGGATGCAATGGCAATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAA
TTGTCATCATCAGTTCAAACTGACATCAATTTGCCATATCTTACAATGGACGCAAGTGGA
CCTAAACACATGAACCTCAAGATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTC
ATTAAACGTACAATTGCACCTTGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCG
GATGTCGGTGAAGTTTTGTTAGTCGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACA
GTTCAAGATATTTTCGGTAGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCA
GTTGGTGCTGCAGTACAAGGTGGTGTTTTGGCTGGAGATGTAACTGATGTACTTTTACTT
GATGTTACACCATTGTCACTTGGTATTGAAACACTTGGCGGCGTCTTTACACGTTTGATC
ACAAGAAACACAACAATTCCAACAAAGAAATCACAAGTATTCTCAACAGCAGCTGATGGA
CAAACACAAGTCGAAATCAAAGTTCACCAGGGTGAACGTGAAATGGCATCTGATAACAAA
TTACTTGGAGCTTTTACACTTGTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAAATC
GAAGTTACATTTGATATTGATGCAAACGGTATTGTACATGTATCAGCTCGTGACAAGGGA
ACTGGCAAGGAACAACAAATTGTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAAATC
GAAAACATGATAAAGAATGCCGAACAATATGCACAAGCTGATAAGACAAAGAAGGATCGA
ATTGAGGCTATCAATCAGGCTGAGTCAATTGTGCACGATACAGAAACAAAAATGGAAGAA
TTCAAAGATCAATTACCAAAGGAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAAGTA
CGAACACTCCTTGCCGATAAAGAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACTGGA
GCTCTACAACAATCATCACTGAAACTCTTCGAAATGGCATACAAAAAAATGGCATCAGAA
TGTGAGAGCTCAGGAAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAGGAAGATAAA
AATTAA

>g10883.t18 Gene=g10883 Length=408
MLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAMAMQRLKEAAEKAKCELSSSVQTDINLPY
LTMDASGPKHMNLKMTRSKLENLVGDLIKRTIAPCQKALSDAEVSKSDVGEVLLVGGMTR
MPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAVQGGVLAGDVTDVLLLDVTPLSLGIETLG
GVFTRLITRNTTIPTKKSQVFSTAADGQTQVEIKVHQGEREMASDNKLLGAFTLVGIPPA
PRGVPQIEVTFDIDANGIVHVSARDKGTGKEQQIVIQSSGGLSKDEIENMIKNAEQYAQA
DKTKKDRIEAINQAESIVHDTETKMEEFKDQLPKEECDKLREEIAKVRTLLADKENADPE
VVRKETGALQQSSLKLFEMAYKKMASECESSGSKSSSEEGGEKKEDKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g10883.t18 Coils Coil Coil 34 54 -
14 g10883.t18 Coils Coil Coil 311 331 -
16 g10883.t18 Coils Coil Coil 337 357 -
11 g10883.t18 Gene3D G3DSA:3.90.640.10 Actin; Chain A 3 80 6.6E-34
12 g10883.t18 Gene3D G3DSA:3.30.420.40 - 81 137 7.5E-10
10 g10883.t18 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 153 281 7.4E-60
13 g10883.t18 Gene3D G3DSA:1.20.1270.10 - 282 385 1.1E-26
18 g10883.t18 MobiDBLite mobidb-lite consensus disorder prediction 386 408 -
2 g10883.t18 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 2 364 1.8E-163
3 g10883.t18 PANTHER PTHR19375:SF409 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 2 364 1.8E-163
7 g10883.t18 PRINTS PR00301 70kDa heat shock protein signature 109 125 1.0E-42
5 g10883.t18 PRINTS PR00301 70kDa heat shock protein signature 140 160 1.0E-42
4 g10883.t18 PRINTS PR00301 70kDa heat shock protein signature 163 182 1.0E-42
6 g10883.t18 PRINTS PR00301 70kDa heat shock protein signature 244 260 1.0E-42
1 g10883.t18 Pfam PF00012 Hsp70 protein 2 381 3.1E-151
17 g10883.t18 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 112 126 -
9 g10883.t18 SUPERFAMILY SSF53067 Actin-like ATPase domain 1 157 5.94E-50
8 g10883.t18 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 159 315 3.53E-63

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values