Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t19 isoform g10883.t19 12438393 12439027
chr_1 g10883 g10883.t19 exon g10883.t19.exon1 12438393 12439027
chr_1 g10883 g10883.t19 cds g10883.t19.CDS1 12438469 12439026
chr_1 g10883 g10883.t19 TTS g10883.t19 12439790 12439790
chr_1 g10883 g10883.t19 TSS g10883.t19 NA NA

Sequences

>g10883.t19 Gene=g10883 Length=635
TTTCGACAACGCAATTGTCAATTTCTTAGTTGATGAATTCAAGAAAGAGCAAGGTATTGA
TATTAGAAAGGATGCAATGGCAATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTG
TGAATTGTCATCATCAGTTCAAACTGACATCAATTTGCCATATCTTACAATGGACGCAAG
TGGACCTAAACACATGAACCTCAAGATGACTCGTTCAAAATTAGAAAATCTTGTAGGAGA
TCTCATTAAACGTACAATTGCACCTTGCCAGAAAGCTTTATCCGATGCCGAAGTATCGAA
ATCGGATGTCGGTGAAGTTTTGTTAGTCGGTGGTATGACAAGAATGCCCAAAGTTCAAAC
AACAGTTCAAGATATTTTCGGTAGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGT
TGCAGTTGGTGCTGCAGTACAAGGTGGTGTTTTGGCTGGAGATGTAACTGATGTACTTTT
ACTTGATGTTACACCATTGTCACTTGGTATTGAAACACTTGGCGGCGTCTTTACACGTTT
GATCACAAGAAACACAACAATTCCAACAAAGAAATCACAAGTATTCTCAACAGCAGCTGA
TGGACAAACACAAGTCGAAATCAAAGTTCACCAGG

>g10883.t19 Gene=g10883 Length=186
MAMQRLKEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRT
IAPCQKALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAA
VQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAADGQTQV
EIKVHQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g10883.t19 Coils Coil Coil 3 23 -
11 g10883.t19 Gene3D G3DSA:3.90.640.10 Actin; Chain A 1 48 1.4E-17
12 g10883.t19 Gene3D G3DSA:3.30.420.40 - 49 106 2.0E-10
10 g10883.t19 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 118 186 3.0E-27
2 g10883.t19 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 186 1.4E-88
3 g10883.t19 PANTHER PTHR19375:SF356 OS09G0491772 PROTEIN 1 186 1.4E-88
4 g10883.t19 PRINTS PR00301 70kDa heat shock protein signature 78 94 9.8E-33
5 g10883.t19 PRINTS PR00301 70kDa heat shock protein signature 109 129 9.8E-33
6 g10883.t19 PRINTS PR00301 70kDa heat shock protein signature 132 151 9.8E-33
1 g10883.t19 Pfam PF00012 Hsp70 protein 1 186 6.7E-83
9 g10883.t19 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 81 95 -
8 g10883.t19 SUPERFAMILY SSF53067 Actin-like ATPase domain 2 126 1.57E-38
7 g10883.t19 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 128 186 1.12E-21

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed