Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t2 isoform g10883.t2 12436979 12439672
chr_1 g10883 g10883.t2 exon g10883.t2.exon1 12436979 12437153
chr_1 g10883 g10883.t2 exon g10883.t2.exon2 12437660 12438215
chr_1 g10883 g10883.t2 cds g10883.t2.CDS1 12437704 12438215
chr_1 g10883 g10883.t2 exon g10883.t2.exon3 12438279 12439524
chr_1 g10883 g10883.t2 cds g10883.t2.CDS2 12438279 12439524
chr_1 g10883 g10883.t2 exon g10883.t2.exon4 12439595 12439672
chr_1 g10883 g10883.t2 cds g10883.t2.CDS3 12439595 12439672
chr_1 g10883 g10883.t2 TTS g10883.t2 12439790 12439790
chr_1 g10883 g10883.t2 TSS g10883.t2 NA NA

Sequences

>g10883.t2 Gene=g10883 Length=2055
TTTTTAATTTAATTTTGTATAATTAAGCATAATGAAAAACACGAAGAATTATTAAGGTTC
AAAAATATAAAAATTTCGTTAAAAATAACTGTCAATTAAGATTCAAAAGACGAGTTTCGA
AAAAAATATTGCGTCACATTGTTCGAATTCGTACTTCGCATTCTGTATAATTCTCTCATT
GGTATTGACTTAGGAACAACAAATTCATGTGTGGCTGTTATGGAAGGAAAACAGGCCAAG
GTCATTGAAAACGCAGAAGGTGCAAGAACAACACCATCTCATGTTGCATTTACAAAAGAC
GGAGAACGTTTAGTCGGTATGCCAGCAAAGAGACAAGCTGTGACAAATTCTGCAAATACC
TTTTATGCTACAAAACGTTTGATTGGACGCCGATTTGAAGATCCCGAGATTAAGAAAGAT
ATGAAGAACTTGTCATATAAAGTAGTCAAAGCTTCAAATGGTGATGCATGGGTTCAATCT
TCAGATGGTAAAGTCTATTCGCCTTCACAAATTGGTGCTTTCGTTTTAATGAAAATGAAG
GAAACTGCAGAAGCATATCTTAATCAACCAGTTAAGAATGCAGTTGTTACTGTTCCTGCA
TATTTTAATGATTCACAACGTCAAGCAACAAAAGATGCTGGTCAAATTTCTGGTCTCAAT
GTTTTGCGTGTTATTAACGAACCAACAGCTGCTGCTTTGGCTTATGGCATGGACAAGAGT
GAAGATAAAATAATTGCTGTTTATGATCTTGGCGGTGGAACATTCGATATTTCAATTCTT
GAAATTCAGAAAGGTGTCTTTGAAGTAAAATCAACAAATGGTGATACAATGTTGGGTGGT
GAGGATTTCGACAACGCAATTGTCAATTTCTTAGTTGATGAATTCAAGAAAGAGCAAGGT
ATTGATATTAGAAAGGATGCAATGGCAATGCAACGTCTTAAAGAAGCAGCTGAAAAAGCA
AAGTGTGAATTGTCATCATCAGTTCAAACTGACATCAATTTGCCATATCTTACAATGGAC
GCAAGTGGACCTAAACACATGAACCTCAAGATGACTCGTTCAAAATTAGAAAATCTTGTA
GGAGATCTCATTAAACGTACAATTGCACCTTGCCAGAAAGCTTTATCCGATGCCGAAGTA
TCGAAATCGGATGTCGGTGAAGTTTTGTTAGTCGGTGGTATGACAAGAATGCCCAAAGTT
CAAACAACAGTTCAAGATATTTTCGGTAGACAACCATCACGTTCAGTTAATCCAGATGAA
GCTGTTGCAGTTGGTGCTGCAGTACAAGGTGGTGTTTTGGCTGGAGATGTAACTGATGTA
CTTTTACTTGATGTTACACCATTGTCACTTGGTATTGAAACACTTGGCGGCGTCTTTACA
CGTTTGATCACAAGAAACACAACAATTCCAACAAAGAAATCACAAGTATTCTCAACAGCA
GCTGATGGACAAACACAAGTCGAAATCAAAGTTCACCAGGGTGAACGTGAAATGGCATCT
GATAACAAATTACTTGGAGCTTTTACACTTGTCGGCATTCCACCTGCTCCACGCGGTGTC
CCACAAATCGAAGTTACATTTGATATTGATGCAAACGGTATTGTACATGTATCAGCTCGT
GACAAGGGAACTGGCAAGGAACAACAAATTGTCATTCAATCATCAGGTGGTCTCAGTAAA
GATGAAATCGAAAACATGATAAAGAATGCCGAACAATATGCACAAGCTGATAAGACAAAG
AAGGATCGAATTGAGGCTATCAATCAGGCTGAGTCAATTGTGCACGATACAGAAACAAAA
ATGGAAGAATTCAAAGATCAATTACCAAAGGAGGAATGCGATAAATTAAGAGAAGAAATT
GCAAAAGTACGAACACTCCTTGCCGATAAAGAAAATGCAGATCCTGAAGTAGTACGTAAA
GAAACTGGAGCTCTACAACAATCATCACTGAAACTCTTCGAAATGGCATACAAAAAAATG
GCATCAGAATGTGAGAGCTCAGGAAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAG
GAAGATAAAAATTAA

>g10883.t2 Gene=g10883 Length=611
MEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLIGRRFE
DPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLNQPVKN
AVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVYDLGGG
TFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAMAMQRL
KEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTIAPCQK
ALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFGRQPSRSVNPDEAVAVGAAVQGGVL
AGDVTDVLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAADGQTQVEIKVHQ
GEREMASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDKGTGKEQQIVIQ
SSGGLSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKMEEFKDQLPKEEC
DKLREEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKMASECESSGSKSSS
EEGGEKKEDKN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
25 g10883.t2 CDD cd11733 HSPA9-like_NBD 1 360 0.0
23 g10883.t2 Coils Coil Coil 237 257 -
22 g10883.t2 Coils Coil Coil 514 534 -
24 g10883.t2 Coils Coil Coil 540 560 -
19 g10883.t2 Gene3D G3DSA:3.30.420.40 - 2 355 3.6E-150
20 g10883.t2 Gene3D G3DSA:3.30.30.30 - 49 101 3.6E-150
18 g10883.t2 Gene3D G3DSA:3.30.420.40 - 167 340 3.6E-150
17 g10883.t2 Gene3D G3DSA:3.90.640.10 Actin; Chain A 206 293 3.6E-150
16 g10883.t2 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 356 484 1.5E-59
21 g10883.t2 Gene3D G3DSA:1.20.1270.10 - 485 588 2.2E-26
4 g10883.t2 Hamap MF_00332 Chaperone protein DnaK [dnaK]. 1 609 32.800129
28 g10883.t2 MobiDBLite mobidb-lite consensus disorder prediction 589 611 -
2 g10883.t2 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 570 3.9E-273
3 g10883.t2 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 1 570 3.9E-273
7 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 13 25 5.2E-72
12 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 36 44 5.2E-72
8 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 121 141 5.2E-72
11 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 180 190 5.2E-72
5 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 312 328 5.2E-72
10 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 343 363 5.2E-72
6 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 366 385 5.2E-72
9 g10883.t2 PRINTS PR00301 70kDa heat shock protein signature 447 463 5.2E-72
1 g10883.t2 Pfam PF00012 Hsp70 protein 1 584 4.3E-259
27 g10883.t2 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 174 187 -
26 g10883.t2 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 315 329 -
15 g10883.t2 SUPERFAMILY SSF53067 Actin-like ATPase domain 3 167 5.18E-52
14 g10883.t2 SUPERFAMILY SSF53067 Actin-like ATPase domain 169 360 4.73E-66
13 g10883.t2 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 362 518 7.72E-63
29 g10883.t2 TIGRFAM TIGR02350 prok_dnaK: chaperone protein DnaK 1 584 1.8E-270

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values