General information for g10883.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heat shock 70 kDa protein cognate 5.
Parent gene
- The parent gene g10883 is located on chr_1: 12439631..12440188 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g10883 | g10883.t21 | isoform | g10883.t21 | 12438775 | 12440188 |
| chr_1 | g10883 | g10883.t21 | exon | g10883.t21.exon1 | 12438775 | 12439528 |
| chr_1 | g10883 | g10883.t21 | cds | g10883.t21.CDS1 | 12439039 | 12439528 |
| chr_1 | g10883 | g10883.t21 | exon | g10883.t21.exon2 | 12439595 | 12440188 |
| chr_1 | g10883 | g10883.t21 | cds | g10883.t21.CDS2 | 12439595 | 12439611 |
| chr_1 | g10883 | g10883.t21 | TTS | g10883.t21 | 12440183 | 12440183 |
| chr_1 | g10883 | g10883.t21 | TSS | g10883.t21 | NA | NA |
Sequences
>g10883.t21 Gene=g10883 Length=1348
AGACAACCATCACGTTCAGTTAATCCAGATGAAGCTGTTGCAGTTGGTGCTGCAGTACAA
GGTGGTGTTTTGGCTGGAGATGTAACTGATGTACTTTTACTTGATGTTACACCATTGTCA
CTTGGTATTGAAACACTTGGCGGCGTCTTTACACGTTTGATCACAAGAAACACAACAATT
CCAACAAAGAAATCACAAGTATTCTCAACAGCAGCTGATGGACAAACACAAGTCGAAATC
AAAGTTCACCAGGGTGAACGTGAAATGGCATCTGATAACAAATTACTTGGAGCTTTTACA
CTTGTCGGCATTCCACCTGCTCCACGCGGTGTCCCACAAATCGAAGTTACATTTGATATT
GATGCAAACGGTATTGTACATGTATCAGCTCGTGACAAGGGAACTGGCAAGGAACAACAA
ATTGTCATTCAATCATCAGGTGGTCTCAGTAAAGATGAAATCGAAAACATGATAAAGAAT
GCCGAACAATATGCACAAGCTGATAAGACAAAGAAGGATCGAATTGAGGCTATCAATCAG
GCTGAGTCAATTGTGCACGATACAGAAACAAAAATGGAAGAATTCAAAGATCAATTACCA
AAGGAGGAATGCGATAAATTAAGAGAAGAAATTGCAAAAGTACGAACACTCCTTGCCGAT
AAAGAAAATGCAGATCCTGAAGTAGTACGTAAAGAAACTGGAGCTCTACAACAATCATCA
CTGAAACTCTTCGAAATGGCATACAAAAAAGTGAATGGCATCAGAATGTGAGAGCTCAGG
AAGCAAATCAAGTAGTGAAGAGGGTGGTGAAAAGAAGGAAGATAAAAATTAAACAAGTTC
AGACAACAACTTAAGAGGATAAATATATTTTCCCAGTAATTTTAAAAAGCATTGCATTAA
ATAAAAATCAGTTTCTGAATTAGAATTAAAACAAATACAAAAAAATAGACTTTAAACAAA
TAAAAAAACTATTAAAGGATCCAAAAAACAATTATTCAATGAATATCAATGTATTGCAAG
GATATAGAGAAAAGAGATATGACATCGACAATAATTGGAAAATAATCCTACAAATTTTCA
TTATTGCACGAGGATTTCAATTAAATCGATCAATTTCTCTTCAATCTTAATTTAATATTA
CCCCAAATGTTTTGTGAACGAAATAAAATTCGTATCTACCTTTAGCTAATTAGTTATGAT
TTTAAGTTAAGGAAAGATTTTTACTAAAATTTTCTAAGAAAATATTTGAAAGAAAAATTG
TAAAGAATGTGGCTAGAGATAGATGTGTTGAATATATAAATAATAATAAATACGAAAAAT
GAGAATTATAGAAAAATTTGAAATGAAA
>g10883.t21 Gene=g10883 Length=168
MASDNKLLGAFTLVGIPPAPRGVPQIEVTFDIDANGIVHVSARDKGTGKEQQIVIQSSGG
LSKDEIENMIKNAEQYAQADKTKKDRIEAINQAESIVHDTETKMEEFKDQLPKEECDKLR
EEIAKVRTLLADKENADPEVVRKETGALQQSSLKLFEMAYKKVNGIRM
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g10883.t21 | Coils | Coil | Coil | 90 | 110 | - |
| 9 | g10883.t21 | Coils | Coil | Coil | 116 | 136 | - |
| 6 | g10883.t21 | Gene3D | G3DSA:2.60.34.10 | Substrate Binding Domain Of DNAk; Chain A | 1 | 60 | 1.1E-24 |
| 7 | g10883.t21 | Gene3D | G3DSA:1.20.1270.10 | - | 61 | 164 | 1.4E-27 |
| 2 | g10883.t21 | PANTHER | PTHR19375 | HEAT SHOCK PROTEIN 70KDA | 1 | 132 | 1.6E-46 |
| 3 | g10883.t21 | PANTHER | PTHR19375:SF419 | BNAA03G55950D PROTEIN | 1 | 132 | 1.6E-46 |
| 1 | g10883.t21 | Pfam | PF00012 | Hsp70 protein | 1 | 160 | 6.0E-44 |
| 5 | g10883.t21 | SUPERFAMILY | SSF100920 | Heat shock protein 70kD (HSP70), peptide-binding domain | 1 | 94 | 9.16E-33 |
| 4 | g10883.t21 | SUPERFAMILY | SSF100934 | Heat shock protein 70kD (HSP70), C-terminal subdomain | 88 | 162 | 3.22E-5 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
