Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t4 TSS g10883.t4 12437137 12437137
chr_1 g10883 g10883.t4 isoform g10883.t4 12437248 12438359
chr_1 g10883 g10883.t4 exon g10883.t4.exon1 12437248 12437337
chr_1 g10883 g10883.t4 cds g10883.t4.CDS1 12437248 12437337
chr_1 g10883 g10883.t4 exon g10883.t4.exon2 12437599 12438215
chr_1 g10883 g10883.t4 cds g10883.t4.CDS2 12437599 12438215
chr_1 g10883 g10883.t4 exon g10883.t4.exon3 12438279 12438359
chr_1 g10883 g10883.t4 cds g10883.t4.CDS3 12438279 12438357
chr_1 g10883 g10883.t4 TTS g10883.t4 NA NA

Sequences

>g10883.t4 Gene=g10883 Length=788
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATCATTCATTTCTCATAAAAACATTCTCATTT
GTCAACAGATCCGAAGGTGTAAAAGGAGCAGTCATTGGTATTGACTTAGGAACAACAAAT
TCATGTGTGGCTGTTATGGAAGGAAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCA
AGAACAACACCATCTCATGTTGCATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCA
GCAAAGAGACAAGCTGTGACAAATTCTGCAAATACCTTTTATGCTACAAAACGTTTGATT
GGACGCCGATTTGAAGATCCCGAGATTAAGAAAGATATGAAGAACTTGTCATATAAAGTA
GTCAAAGCTTCAAATGGTGATGCATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCT
TCACAAATTGGTGCTTTCGTTTTAATGAAAATGAAGGAAACTGCAGAAGCATATCTTAAT
CAACCAGTTAAGAATGCAGTTGTTACTGTTCCTGCATATTTTAATGATTCACAACGTCAA
GCAACAAAAGATGCTGGTCAAATTTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCA
ACAGCTGCTGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTAT
GATCTTGGCGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAA
GTAAAATC

>g10883.t4 Gene=g10883 Length=262
MLSATKHLLKNTNITQTLMNTRNLSSLMKNHSFLIKTFSFVNRSEGVKGAVIGIDLGTTN
SCVAVMEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLI
GRRFEDPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLN
QPVKNAVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVY
DLGGGTFDISILEIQKGVFEVK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g10883.t4 Gene3D G3DSA:3.30.30.30 - 114 166 3.4E-26
13 g10883.t4 Gene3D G3DSA:3.30.420.40 - 237 262 2.7E-11
2 g10883.t4 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 38 262 1.2E-108
3 g10883.t4 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 38 262 1.2E-108
7 g10883.t4 PRINTS PR00301 70kDa heat shock protein signature 50 63 2.8E-34
4 g10883.t4 PRINTS PR00301 70kDa heat shock protein signature 78 90 2.8E-34
5 g10883.t4 PRINTS PR00301 70kDa heat shock protein signature 101 109 2.8E-34
8 g10883.t4 PRINTS PR00301 70kDa heat shock protein signature 186 206 2.8E-34
6 g10883.t4 PRINTS PR00301 70kDa heat shock protein signature 245 255 2.8E-34
1 g10883.t4 Pfam PF00012 Hsp70 protein 51 262 3.9E-109
11 g10883.t4 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 54 61 -
12 g10883.t4 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 239 252 -
9 g10883.t4 SUPERFAMILY SSF53067 Actin-like ATPase domain 51 232 2.04E-64
10 g10883.t4 SUPERFAMILY SSF53067 Actin-like ATPase domain 233 260 8.78E-7

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values