Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 5.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10883 g10883.t8 TSS g10883.t8 12437137 12437137
chr_1 g10883 g10883.t8 isoform g10883.t8 12437248 12438775
chr_1 g10883 g10883.t8 exon g10883.t8.exon1 12437248 12437337
chr_1 g10883 g10883.t8 exon g10883.t8.exon2 12437439 12437468
chr_1 g10883 g10883.t8 exon g10883.t8.exon3 12437637 12438215
chr_1 g10883 g10883.t8 cds g10883.t8.CDS1 12437704 12438215
chr_1 g10883 g10883.t8 exon g10883.t8.exon4 12438279 12438775
chr_1 g10883 g10883.t8 cds g10883.t8.CDS2 12438279 12438774
chr_1 g10883 g10883.t8 TTS g10883.t8 12439790 12439790

Sequences

>g10883.t8 Gene=g10883 Length=1196
ATGTTGTCAGCAACAAAGCATTTATTGAAAAACACAAATATCACACAAACTCTAATGAAC
ACCAGAAATCTTTCGAGTTTGATGAAAAATTCACTTTAACCGATTGCAATGTAGATATAA
ATCCGAAGGTGTAAAAGGAGCAGTCATTGGTATTGACTTAGGAACAACAAATTCATGTGT
GGCTGTTATGGAAGGAAAACAGGCCAAGGTCATTGAAAACGCAGAAGGTGCAAGAACAAC
ACCATCTCATGTTGCATTTACAAAAGACGGAGAACGTTTAGTCGGTATGCCAGCAAAGAG
ACAAGCTGTGACAAATTCTGCAAATACCTTTTATGCTACAAAACGTTTGATTGGACGCCG
ATTTGAAGATCCCGAGATTAAGAAAGATATGAAGAACTTGTCATATAAAGTAGTCAAAGC
TTCAAATGGTGATGCATGGGTTCAATCTTCAGATGGTAAAGTCTATTCGCCTTCACAAAT
TGGTGCTTTCGTTTTAATGAAAATGAAGGAAACTGCAGAAGCATATCTTAATCAACCAGT
TAAGAATGCAGTTGTTACTGTTCCTGCATATTTTAATGATTCACAACGTCAAGCAACAAA
AGATGCTGGTCAAATTTCTGGTCTCAATGTTTTGCGTGTTATTAACGAACCAACAGCTGC
TGCTTTGGCTTATGGCATGGACAAGAGTGAAGATAAAATAATTGCTGTTTATGATCTTGG
CGGTGGAACATTCGATATTTCAATTCTTGAAATTCAGAAAGGTGTCTTTGAAGTAAAATC
AACAAATGGTGATACAATGTTGGGTGGTGAGGATTTCGACAACGCAATTGTCAATTTCTT
AGTTGATGAATTCAAGAAAGAGCAAGGTATTGATATTAGAAAGGATGCAATGGCAATGCA
ACGTCTTAAAGAAGCAGCTGAAAAAGCAAAGTGTGAATTGTCATCATCAGTTCAAACTGA
CATCAATTTGCCATATCTTACAATGGACGCAAGTGGACCTAAACACATGAACCTCAAGAT
GACTCGTTCAAAATTAGAAAATCTTGTAGGAGATCTCATTAAACGTACAATTGCACCTTG
CCAGAAAGCTTTATCCGATGCCGAAGTATCGAAATCGGATGTCGGTGAAGTTTTGTTAGT
CGGTGGTATGACAAGAATGCCCAAAGTTCAAACAACAGTTCAAGATATTTTCGGTA

>g10883.t8 Gene=g10883 Length=336
MEGKQAKVIENAEGARTTPSHVAFTKDGERLVGMPAKRQAVTNSANTFYATKRLIGRRFE
DPEIKKDMKNLSYKVVKASNGDAWVQSSDGKVYSPSQIGAFVLMKMKETAEAYLNQPVKN
AVVTVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGMDKSEDKIIAVYDLGGG
TFDISILEIQKGVFEVKSTNGDTMLGGEDFDNAIVNFLVDEFKKEQGIDIRKDAMAMQRL
KEAAEKAKCELSSSVQTDINLPYLTMDASGPKHMNLKMTRSKLENLVGDLIKRTIAPCQK
ALSDAEVSKSDVGEVLLVGGMTRMPKVQTTVQDIFG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g10883.t8 Coils Coil Coil 237 257 -
15 g10883.t8 Gene3D G3DSA:3.30.420.40 - 2 166 9.4E-141
16 g10883.t8 Gene3D G3DSA:3.30.30.30 - 49 101 9.4E-141
14 g10883.t8 Gene3D G3DSA:3.30.420.40 - 167 336 9.4E-141
13 g10883.t8 Gene3D G3DSA:3.90.640.10 Actin; Chain A 206 293 9.4E-141
2 g10883.t8 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 1 336 4.4E-163
3 g10883.t8 PANTHER PTHR19375:SF451 HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIAL 1 336 4.4E-163
5 g10883.t8 PRINTS PR00301 70kDa heat shock protein signature 13 25 6.2E-35
8 g10883.t8 PRINTS PR00301 70kDa heat shock protein signature 36 44 6.2E-35
7 g10883.t8 PRINTS PR00301 70kDa heat shock protein signature 121 141 6.2E-35
6 g10883.t8 PRINTS PR00301 70kDa heat shock protein signature 180 190 6.2E-35
4 g10883.t8 PRINTS PR00301 70kDa heat shock protein signature 312 328 6.2E-35
1 g10883.t8 Pfam PF00012 Hsp70 protein 1 336 3.6E-163
12 g10883.t8 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 174 187 -
11 g10883.t8 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 315 329 -
10 g10883.t8 SUPERFAMILY SSF53067 Actin-like ATPase domain 3 167 1.42E-52
9 g10883.t8 SUPERFAMILY SSF53067 Actin-like ATPase domain 169 336 2.11E-56

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values