Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit DAD1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g10886 g10886.t1 TSS g10886.t1 12445974 12445974
chr_1 g10886 g10886.t1 isoform g10886.t1 12446067 12446465
chr_1 g10886 g10886.t1 exon g10886.t1.exon1 12446067 12446274
chr_1 g10886 g10886.t1 cds g10886.t1.CDS1 12446067 12446274
chr_1 g10886 g10886.t1 exon g10886.t1.exon2 12446335 12446465
chr_1 g10886 g10886.t1 cds g10886.t1.CDS2 12446335 12446465
chr_1 g10886 g10886.t1 TTS g10886.t1 12446574 12446574

Sequences

>g10886.t1 Gene=g10886 Length=339
ATGGTTGCATTAAGTCAAGTGCTTGTCAAATTTTATGAAGAATATCAAAATACTACGCCA
AAGAAGCTAAAAATTGTAGATGCTTATTTAACATACATTTTATTAACTGGGATTTTACAA
TTTGTTTACTGCTGTTTAGTGGGCACTTTTCCATTCAATTCTTTTCTTTCTGGCTTCATC
TCATGTGTTAGCTGCTTTGTTCTTGGAGTATGTCTACGTCTTCAGTCTAATCCACAAAAT
AAACTTCAATTTCAAGGCATTTCACCAGAGAGAGGATTTGCAGATTTCATTTTTGCTCAT
ATCGTATTACATTTAGTTGTAGTCAATTTTATCGGTTAA

>g10886.t1 Gene=g10886 Length=112
MVALSQVLVKFYEEYQNTTPKKLKIVDAYLTYILLTGILQFVYCCLVGTFPFNSFLSGFI
SCVSCFVLGVCLRLQSNPQNKLQFQGISPERGFADFIFAHIVLHLVVVNFIG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g10886.t1 PANTHER PTHR10705 DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1 4 112 3.8E-43
3 g10886.t1 PANTHER PTHR10705:SF0 DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1 4 112 3.8E-43
14 g10886.t1 PIRSF PIRSF005588 DAD1_Ost2 2 112 3.1E-48
1 g10886.t1 Pfam PF02109 DAD family 5 112 8.6E-47
8 g10886.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 28 -
12 g10886.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 29 49 -
10 g10886.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 50 54 -
13 g10886.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 55 72 -
7 g10886.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 73 92 -
11 g10886.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 93 111 -
9 g10886.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 112 112 -
5 g10886.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 28 50 -
6 g10886.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 55 72 -
4 g10886.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 92 111 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0008250 oligosaccharyltransferase complex CC
GO:0016021 integral component of membrane CC

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values