Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 313b1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1091 g1091.t1 isoform g1091.t1 8081892 8083612
chr_3 g1091 g1091.t1 exon g1091.t1.exon1 8081892 8082485
chr_3 g1091 g1091.t1 cds g1091.t1.CDS1 8081892 8082485
chr_3 g1091 g1091.t1 exon g1091.t1.exon2 8082617 8082887
chr_3 g1091 g1091.t1 cds g1091.t1.CDS2 8082617 8082887
chr_3 g1091 g1091.t1 exon g1091.t1.exon3 8082996 8083612
chr_3 g1091 g1091.t1 cds g1091.t1.CDS3 8082996 8083612
chr_3 g1091 g1091.t1 TSS g1091.t1 NA NA
chr_3 g1091 g1091.t1 TTS g1091.t1 NA NA

Sequences

>g1091.t1 Gene=g1091 Length=1482
ATGTACATAAGGGAAACTTTATTTGCAATAATTAGCACTTTTATTTTTCACTTGATTTGG
AAATGGAAAAGATTTTTTTATCTTGCCAGTAAAATTCCAAGAAGTCAGTTTGATTTTTCA
TGGCAAGGAGTTAAAGATGTGATCAATGCTGATAACAGAATTGCATTTAAAATGATTTAT
GAAAGTTTCGATGGTATTGATGGATTAGCAAAGACTTGGCTTGGTCCGATGCTGTTTGTG
ATTATAAGTTCACCAGAAGATGTAAAAATTGTGATGAATTCAAGAGATTGCTTTGATAAG
CCTTATTTTGTACGCTGGCCAGGCTCAATTCCGAAAGGATCACTTTTTGGAAGCCTTGAT
TATTGGCATAAACATCGAAAAATTCTCGATCCATACTTTGGACAGCAAAAATTGAAAAAG
TTTGCGACTTTGTTTGATGAAAAATCAAAAATTTTAACTGGAAACGTCGCACAAAAACTT
AACCAAGGTGATTTTGATATTTTTCATTACATGACAGCACTGACTCTTGAAATTATTTTA
AACGCTATGGAACTTGACGTTGACATTCAAAATCTTGAAACTAAATTGAGAGATGTGACA
ATCAAAGGATTAGAAACAGCAAGCAAATTAGTAGCAATTCGAATGTTTAAATTTTGGCTT
CATCCTGATTTCATTTACAAACGAACGAAAATGTACATTGATGAGACAAAGGCAAAGTCA
TACGCTTATACATATGCAGAAGATGTGATAGACAACATTCGTAAAAAGCTCAATGACGAT
ATTGATGAGGAAGATAAACCAACAACTTTTATGCGTGCACTTATGAGTCCAAAATTTAAT
TTGTCCGAAAGTGAAATTAGTGATGAAATCAAAACTCTTTTAGTTGCTGCACAAGATACG
ACTGGAATTTCAATGTCAGCAACACTTTTACTACTTGCAATGCACAAAGATAAACAAGCA
AAAGTCATTGATGAAATGCATCGAGTTATTGGCAATAATCCTGATATACCGTTTACAGAT
TTTGATAAGCTCAATGAATTGCATTATCTTGAAATGGTCATTAATGAAGCATTAAGACTC
ATGCCAGTTGTTCCTTTTGTATTTCGATCAGTTGACAGTGAAATTACTTCACATGAAGGT
TATATCATTCCACCAAATGCAAATCTCATTATTCCGATCTTTAAAATTCAACGAAATAAA
ACATTTTGGGGTGAAGATGCTGATAAGTTCAGACCTGAAAGATTTGAGAAAGAAAACATC
AAAAAAGTCAATTCTTATGCTTTTCTACCTTTTACAAGAGGACCACGAATGTGTTTAGGT
TGGCGATATGCAATGATGTTGATGAAAATTCAACTTTGTAACTTTCTGGTGCGCTATGAA
GTTGACACAAGTTTAAAATATGAAGAATTAGAGTTTGAGTTAAATGTGACCTTGAATATT
TGTCAAGGTTTTAAAATTAGCATTAAAGAAAGAAACATTTGA

>g1091.t1 Gene=g1091 Length=493
MYIRETLFAIISTFIFHLIWKWKRFFYLASKIPRSQFDFSWQGVKDVINADNRIAFKMIY
ESFDGIDGLAKTWLGPMLFVIISSPEDVKIVMNSRDCFDKPYFVRWPGSIPKGSLFGSLD
YWHKHRKILDPYFGQQKLKKFATLFDEKSKILTGNVAQKLNQGDFDIFHYMTALTLEIIL
NAMELDVDIQNLETKLRDVTIKGLETASKLVAIRMFKFWLHPDFIYKRTKMYIDETKAKS
YAYTYAEDVIDNIRKKLNDDIDEEDKPTTFMRALMSPKFNLSESEISDEIKTLLVAAQDT
TGISMSATLLLLAMHKDKQAKVIDEMHRVIGNNPDIPFTDFDKLNELHYLEMVINEALRL
MPVVPFVFRSVDSEITSHEGYIIPPNANLIIPIFKIQRNKTFWGEDADKFRPERFEKENI
KKVNSYAFLPFTRGPRMCLGWRYAMMLMKIQLCNFLVRYEVDTSLKYEELEFELNVTLNI
CQGFKISIKERNI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g1091.t1 Coils Coil Coil 182 202 -
14 g1091.t1 Gene3D G3DSA:1.10.630.10 Cytochrome p450 32 493 4.6E-89
2 g1091.t1 PANTHER PTHR24291:SF140 CYTOCHROME P450 4C3-RELATED 8 491 1.1E-80
3 g1091.t1 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 8 491 1.1E-80
8 g1091.t1 PRINTS PR00463 E-class P450 group I signature 285 302 1.7E-12
10 g1091.t1 PRINTS PR00385 P450 superfamily signature 296 313 7.0E-9
4 g1091.t1 PRINTS PR00463 E-class P450 group I signature 305 331 1.7E-12
7 g1091.t1 PRINTS PR00463 E-class P450 group I signature 351 369 1.7E-12
9 g1091.t1 PRINTS PR00385 P450 superfamily signature 352 363 7.0E-9
5 g1091.t1 PRINTS PR00463 E-class P450 group I signature 428 438 1.7E-12
12 g1091.t1 PRINTS PR00385 P450 superfamily signature 429 438 7.0E-9
6 g1091.t1 PRINTS PR00463 E-class P450 group I signature 438 461 1.7E-12
11 g1091.t1 PRINTS PR00385 P450 superfamily signature 438 449 7.0E-9
1 g1091.t1 Pfam PF00067 Cytochrome P450 71 471 1.3E-69
17 g1091.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
18 g1091.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 22 -
16 g1091.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 23 493 -
13 g1091.t1 SUPERFAMILY SSF48264 Cytochrome P450 57 491 3.93E-86

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed