General information for g11168.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Chymotrypsin-2.
Parent gene
- The parent gene g11168 is located on chr_1: 14383532..14384461 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11168 | g11168.t1 | isoform | g11168.t1 | 14383532 | 14384461 |
| chr_1 | g11168 | g11168.t1 | exon | g11168.t1.exon1 | 14383532 | 14383758 |
| chr_1 | g11168 | g11168.t1 | cds | g11168.t1.CDS1 | 14383532 | 14383758 |
| chr_1 | g11168 | g11168.t1 | exon | g11168.t1.exon2 | 14383813 | 14384214 |
| chr_1 | g11168 | g11168.t1 | cds | g11168.t1.CDS2 | 14383813 | 14384214 |
| chr_1 | g11168 | g11168.t1 | exon | g11168.t1.exon3 | 14384269 | 14384334 |
| chr_1 | g11168 | g11168.t1 | cds | g11168.t1.CDS3 | 14384269 | 14384334 |
| chr_1 | g11168 | g11168.t1 | exon | g11168.t1.exon4 | 14384389 | 14384461 |
| chr_1 | g11168 | g11168.t1 | cds | g11168.t1.CDS4 | 14384389 | 14384461 |
| chr_1 | g11168 | g11168.t1 | TTS | g11168.t1 | 14384522 | 14384522 |
| chr_1 | g11168 | g11168.t1 | TSS | g11168.t1 | NA | NA |
Sequences
>g11168.t1 Gene=g11168 Length=768
ATGATTGCATTTAAAATCATTGTCTTGATTTGTATTTTGCATGGAGTTTGTTCAAAAAAT
ATTGATTCAGGTGAACGTAAAGTTGATAACAGAATTGTAGGAGGTCAGGATGCTGAAAAA
GATTCAGCTCCATATCAGGTCTCACTTCAAACAAAATCGCGTCACAATTGTGGAGGTGCG
ATCATTGATTCTCGTTTTATTTTAACAGCAGCACATTGTCTTTATGGGAGGTTTGCAAGC
GACTATACAGTCATGGTTGGAAGTAATAAATTAAGTGAAGCTTCAACATTTTATAAACCA
GATAAATTAATAATTCATAGCCGCTATAATCAACCGACATATCATAACGATATTGGACTA
ATAAGATTAAAAGAAGCAATACAATTTACTGATAACATCAAACCAATTGACTATGAATGG
CGAGAAGTACCTGAAAATGCATCGATTCGTTTAACAGGTTGGGGTAAGCTATCAACTATC
GGAGCGATCCCTGATAAACTCCAAGAAATTGAATTAAAACATATTAGTTATGAAGAATGT
AAAAAGAGACATGATGATGACGAAAATGTCGATTATGGTCATTTATGTACATTTAATAAA
GCTGGTGAAGCAGCTTGTAATGGAGATTCTGGATCACCTTTAACTTATAATGGAAAAGTT
GTAGCAGTTGTCAATTGGGGAGTACCATGCGGGCGTGGATATCCCGACGTACATGCTCGT
GTTGCATATTATCATGATTGGATTCGTACTACTATCAATTCCAATTAA
>g11168.t1 Gene=g11168 Length=255
MIAFKIIVLICILHGVCSKNIDSGERKVDNRIVGGQDAEKDSAPYQVSLQTKSRHNCGGA
IIDSRFILTAAHCLYGRFASDYTVMVGSNKLSEASTFYKPDKLIIHSRYNQPTYHNDIGL
IRLKEAIQFTDNIKPIDYEWREVPENASIRLTGWGKLSTIGAIPDKLQEIELKHISYEEC
KKRHDDDENVDYGHLCTFNKAGEAACNGDSGSPLTYNGKVVAVVNWGVPCGRGYPDVHAR
VAYYHDWIRTTINSN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g11168.t1 | CDD | cd00190 | Tryp_SPc | 32 | 251 | 8.55595E-80 |
| 9 | g11168.t1 | Gene3D | G3DSA:2.40.10.10 | - | 33 | 242 | 3.4E-67 |
| 10 | g11168.t1 | Gene3D | G3DSA:2.40.10.10 | - | 44 | 248 | 3.4E-67 |
| 2 | g11168.t1 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 8 | 252 | 3.6E-65 |
| 3 | g11168.t1 | PANTHER | PTHR24276:SF63 | RE08710P | 8 | 252 | 3.6E-65 |
| 5 | g11168.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 58 | 73 | 4.3E-13 |
| 4 | g11168.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 113 | 127 | 4.3E-13 |
| 6 | g11168.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 203 | 215 | 4.3E-13 |
| 1 | g11168.t1 | Pfam | PF00089 | Trypsin | 32 | 248 | 1.3E-57 |
| 12 | g11168.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 13 | g11168.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 14 | g11168.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 13 | - |
| 15 | g11168.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 11 | g11168.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 255 | - |
| 18 | g11168.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 68 | 73 | - |
| 19 | g11168.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 204 | 215 | - |
| 20 | g11168.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 32 | 253 | 29.169 |
| 17 | g11168.t1 | SMART | SM00020 | trypsin_2 | 31 | 248 | 8.4E-76 |
| 7 | g11168.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 8 | 254 | 1.4E-74 |
| 8 | g11168.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
