General information for g11200.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-1,3/1,6-mannosyltransferase ALG2.
Parent gene
- The parent gene g11200 is located on chr_1: 14550191..14551483 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11200 | g11200.t3 | TSS | g11200.t3 | 14548728 | 14548728 |
| chr_1 | g11200 | g11200.t3 | isoform | g11200.t3 | 14548775 | 14550191 |
| chr_1 | g11200 | g11200.t3 | exon | g11200.t3.exon1 | 14548775 | 14548869 |
| chr_1 | g11200 | g11200.t3 | exon | g11200.t3.exon2 | 14548922 | 14550191 |
| chr_1 | g11200 | g11200.t3 | cds | g11200.t3.CDS1 | 14548968 | 14550191 |
| chr_1 | g11200 | g11200.t3 | TTS | g11200.t3 | 14550204 | 14550204 |
Sequences
>g11200.t3 Gene=g11200 Length=1365
GACTTTTATTTTTTTCTTTAAAAAATAAGGAATTATTGGCTCAAATAAGAGTATTTATTT
AAAAGTAATTATTTATCAAACATTTATCAATAAAGCCACGTAAATTTTTTTTCATAGATT
TGATAAACACAAGACAAAAGTATGCGAGTACTTTTTATGCACCCAGATTTGGGTATTGGC
GGAGCTGAAAGGCTTGTTGTCGATGCAGCACTCGCTTTACAAAATAAAGGTCATACAGTA
AGCTTTCTCACAAACCATCATGATCCATCACATTGCTTTGAAGAGACAAGAAATGGAAAA
TTAAAAGTTCGAGTTGTCGGCGATTGGATTCCGAGAAGTATTTTTGGCAAATTTTATGCA
TTCTGCGCATATTTTAGAATGATGTATGCAGCTTTTTACGTCGTATTATTTCAATCAAAA
GAAGAAAAAATCGATTTGATCTTTTGTGATCTCATATCATTAGGAATACCAATTTTAAAG
TTTGCCGAACACACACCCAAAATTCTTTTTTATTGTCATTTTCCTGATCAACTTCTCACA
AAACAAGGAGGCTTTTTAAAAAGAATTTATCGAATGCCATTAAATTATTTAGAAGAGAAA
ACGACTGGTGCTGCTGATGGCGTTTTAGTTAATAGCAAGTTTACAAGTAGAATATTTAGT
GAGACATTTAAAACACTTAATATTACTCCAGAAGTTCTCTATCCTTCTCTCGTTACCAGT
TCTTTTGATATAACTTCTCCAATTGATCATTCGGTAACTGACATCGATGATAATGCCACA
ATTATTTTATCAATAAATCGATATGAAAGAAAGAAAAATCTCCCTTTAGCATTAAAAGCA
TTCAAGTTACTTGAAGGAAATCTTACAAAACATGAATGGGATAGAGCTCATTTAATTTTA
GCTGGTGGTTATGATTCAAGAAATTTGGAAAATATTGAGCATTACGATGAACTTTCAGTT
ATTTGTGAAAACCTTGGATTAAAAGATAAAGTAACATTGTTAAGGTCACCAAGTGATGAG
ATAAAAGTTAAATTGCTAAATCGATGTCGAATGTTATTCTATACACCAACAAATGAGCAT
TTTGGAATTGTCCCTTTAGAAGCTATGTATTTGGCTCGACCTGTGATTGCAGTAAATAGT
GGTGGACCTACTGAAACTATCGTTCACGAACAGACTGGTTTTTTGTGTGAATCAACACCA
GAAGATTTTGCAAATGCAGCAACTCTCATTATCAAAGATCAGAAACTAGGTGAAAAAATG
GGAGACATGGGAAGAAAACGTGTTCAACAAAGATTCTCATTTGAAGCATTCACTGAAAAA
CTTAATCAAATTGTAATCAATTTAATAAATAGACGTAGTACATAG
>g11200.t3 Gene=g11200 Length=407
MRVLFMHPDLGIGGAERLVVDAALALQNKGHTVSFLTNHHDPSHCFEETRNGKLKVRVVG
DWIPRSIFGKFYAFCAYFRMMYAAFYVVLFQSKEEKIDLIFCDLISLGIPILKFAEHTPK
ILFYCHFPDQLLTKQGGFLKRIYRMPLNYLEEKTTGAADGVLVNSKFTSRIFSETFKTLN
ITPEVLYPSLVTSSFDITSPIDHSVTDIDDNATIILSINRYERKKNLPLALKAFKLLEGN
LTKHEWDRAHLILAGGYDSRNLENIEHYDELSVICENLGLKDKVTLLRSPSDEIKVKLLN
RCRMLFYTPTNEHFGIVPLEAMYLARPVIAVNSGGPTETIVHEQTGFLCESTPEDFANAA
TLIIKDQKLGEKMGDMGRKRVQQRFSFEAFTEKLNQIVINLINRRST
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g11200.t3 | CDD | cd03805 | GT4_ALG2-like | 1 | 394 | 0.0 |
| 7 | g11200.t3 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 1 | 204 | 5.9E-12 |
| 6 | g11200.t3 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 205 | 385 | 3.1E-41 |
| 3 | g11200.t3 | PANTHER | PTHR45918 | ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2 | 1 | 404 | 1.0E-167 |
| 4 | g11200.t3 | PANTHER | PTHR45918:SF1 | ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2 | 1 | 404 | 1.0E-167 |
| 2 | g11200.t3 | Pfam | PF13439 | Glycosyltransferase Family 4 | 12 | 177 | 2.3E-14 |
| 1 | g11200.t3 | Pfam | PF00534 | Glycosyl transferases group 1 | 202 | 380 | 6.0E-37 |
| 9 | g11200.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 70 | - |
| 11 | g11200.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 90 | - |
| 8 | g11200.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 91 | 96 | - |
| 12 | g11200.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 97 | 115 | - |
| 10 | g11200.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 116 | 407 | - |
| 5 | g11200.t3 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 3 | 403 | 1.22E-64 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006486 | protein glycosylation | BP |
| GO:0016757 | glycosyltransferase activity | MF |
| GO:0004378 | GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03843 (alpha-1,3/alpha-1,6-mannosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
