Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Myosin regulatory light chain sqh.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11383 g11383.t47 TTS g11383.t47 15878931 15878931
chr_1 g11383 g11383.t47 isoform g11383.t47 15879154 15880834
chr_1 g11383 g11383.t47 exon g11383.t47.exon1 15879154 15879261
chr_1 g11383 g11383.t47 exon g11383.t47.exon2 15879344 15879709
chr_1 g11383 g11383.t47 cds g11383.t47.CDS1 15879485 15879709
chr_1 g11383 g11383.t47 exon g11383.t47.exon3 15879796 15879915
chr_1 g11383 g11383.t47 cds g11383.t47.CDS2 15879796 15879906
chr_1 g11383 g11383.t47 exon g11383.t47.exon4 15880716 15880834
chr_1 g11383 g11383.t47 TSS g11383.t47 15880819 15880819

Sequences

>g11383.t47 Gene=g11383 Length=713
ATGTAGTGTAGAAGTTGATTTCGAGAATAAAAACACTTTTTTTTGATTGAAAAATTTTAA
TTGATTTTGCATCTTATAGTGTTTTAACTAATTTGTATTGAATTTAATATAATATTTAGA
GAATCATCATGTCGTCACGAAAAACAGCAGGCCGACGTGCTACTACAAAAAAACGAGCTC
AACGAGCCACTTCAAATGTCTTTGCCATGTTCGATCAAGCCCAGATTGCAGAGTTCAAAG
AAGCTTTCAATATGATTGATCAAAATCGCGATGGATTCATTGAAAAAGAAGATCTTCATG
ATATGCTTGCTTCACTTGGAAAGAATCCAACAGATGACTATTTGGAGTCAATGATGAATG
AAGCTCCTGGTCCAATTAATTTTACAATGTTTCTCACTTTATTTGGAGAAAGATTACAAG
GCAAGAATTGTGTCACTTCTACATTAGAGTATTATTATCAATAATTTTTCGCTCCAGGCA
CAGACCCTGAAGATGTAATTAAAAATGCATTCGGATGCTTTGATGAAGACAATAATGGCC
ATATTTCTGAGGATCGTCTGAGAGAGTTATTAACTACAATGGGTGATCGTTTTACTGATG
AAGAGGTTGACGAAATGTATCGTGAAGCGCCAATTAAAAACAATCTTTTTGACTATATCG
AATTCACAAGAATCTTAAAACATGGCGCTAAAGACAATGAGAATGAACAATAA

>g11383.t47 Gene=g11383 Length=111
MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFIEKEDLHDML
ASLGKNPTDDYLESMMNEAPGPINFTMFLTLFGERLQGKNCVTSTLEYYYQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g11383.t47 CDD cd00051 EFh 35 79 2.31633E-10
5 g11383.t47 Gene3D G3DSA:1.10.238.10 - 16 97 9.8E-27
2 g11383.t47 PANTHER PTHR23049 MYOSIN REGULATORY LIGHT CHAIN 2 1 100 1.9E-51
3 g11383.t47 PANTHER PTHR23049:SF57 MYOSIN REGULATORY LIGHT CHAIN 12A 1 100 1.9E-51
1 g11383.t47 Pfam PF13405 EF-hand domain 35 64 1.4E-7
7 g11383.t47 ProSitePatterns PS00018 EF-hand calcium-binding domain. 44 56 -
9 g11383.t47 ProSiteProfiles PS50222 EF-hand calcium-binding domain profile. 31 66 15.49
8 g11383.t47 SMART SM00054 efh_1 35 63 5.6E-7
4 g11383.t47 SUPERFAMILY SSF47473 EF-hand 28 100 1.07E-19

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005509 calcium ion binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values