Gene loci information

Transcript annotation

  • This transcript has been annotated as MAP kinase-activated protein kinase 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11439 g11439.t1 TTS g11439.t1 16249170 16249170
chr_1 g11439 g11439.t1 isoform g11439.t1 16249383 16251899
chr_1 g11439 g11439.t1 exon g11439.t1.exon1 16249383 16249551
chr_1 g11439 g11439.t1 cds g11439.t1.CDS1 16249383 16249551
chr_1 g11439 g11439.t1 exon g11439.t1.exon2 16249611 16249836
chr_1 g11439 g11439.t1 cds g11439.t1.CDS2 16249611 16249836
chr_1 g11439 g11439.t1 exon g11439.t1.exon3 16249900 16249988
chr_1 g11439 g11439.t1 cds g11439.t1.CDS3 16249900 16249988
chr_1 g11439 g11439.t1 exon g11439.t1.exon4 16250046 16250139
chr_1 g11439 g11439.t1 cds g11439.t1.CDS4 16250046 16250139
chr_1 g11439 g11439.t1 exon g11439.t1.exon5 16250681 16250808
chr_1 g11439 g11439.t1 cds g11439.t1.CDS5 16250681 16250808
chr_1 g11439 g11439.t1 exon g11439.t1.exon6 16251190 16251250
chr_1 g11439 g11439.t1 cds g11439.t1.CDS6 16251190 16251250
chr_1 g11439 g11439.t1 exon g11439.t1.exon7 16251311 16251379
chr_1 g11439 g11439.t1 cds g11439.t1.CDS7 16251311 16251379
chr_1 g11439 g11439.t1 exon g11439.t1.exon8 16251437 16251518
chr_1 g11439 g11439.t1 cds g11439.t1.CDS8 16251437 16251518
chr_1 g11439 g11439.t1 exon g11439.t1.exon9 16251585 16251646
chr_1 g11439 g11439.t1 cds g11439.t1.CDS9 16251585 16251646
chr_1 g11439 g11439.t1 exon g11439.t1.exon10 16251773 16251899
chr_1 g11439 g11439.t1 cds g11439.t1.CDS10 16251773 16251899
chr_1 g11439 g11439.t1 TSS g11439.t1 16252138 16252138

Sequences

>g11439.t1 Gene=g11439 Length=1107
ATGTTTAAAATTAATACAAATACGACTAATCCACCTCAAATGAGTACATCACAGAATTTC
ACTTTGGTTCCGAAAACAACAAGAATCACTGACGAATATGAGATATCGAACAATGTTTTG
GGATTAGGCATAAATGGAAAAGTTGTGCAATGCACAAGAAAAAGTGATAATACTAAATAC
GCATTGAAGGTACTACATGATAATAGCAAAGCACGGAGAGAAGTGGAATTGCATTGGCGT
GTTAGTGGTTGTCGACATATTGTGAATATTGTTGATGTTTTTGAGAACACTTATGGTGGC
ACAAAATGTTTGTTAGTTGTAATGGAACTCATGACTGGTGGTGAGCTCTTCAATCGTATC
CAAGAACGTCAAGATGGACCTTTTACTGAACGTGAAGCTGCACAAATCATGCATGAGATA
TGCATTGCATTGAAATATCTTCATGATCGAAATATTGCTCATAGAGATTTAAAGCCAGAA
AATCTACTTTACTCAAGTCAAGATTGTGATGCGATTATTAAATTAACTGAGACACACATA
AAAGACACGCTCCAGACACCTTGTTATACTCCTTATTATGCTGCTCCAGAAATTCTAGGA
CCAGAAAAATACGATCGTTCTTGTGACTTATGGTCGTTGGGAGTGATAATGTATATTCTG
CTTTGCGGTTTCCCTCCATTTTATAGCAACCACGGTCTTGCCATTTCACCAGGTATGAAA
AATCGCATTCGAACTGGTCAATATGATTTTCCATTGCCCGAGTGGCAAAACGTCAGTAAT
GATGCTAAAGATTTGATTCGAGGAATGTTGAATGTAAACCCCAATGAACGACTTACTATT
GACGAAGTAATGAAAAATAATTGGATCGCACAATATACTAACGTTCCCCAAACACCTTTA
CACACAAATCGTGTCTTGAAAGAAGAAGAACAACAATGGCCTGAAGTACAAGAAGAAATG
AATCGTTCACTTGCTAGCATGAGAGTTGATTATGGCTTAGTACTCAAAAACATTGATAAC
TCGAGTAATTCACTTTTAAATAAACGCAAAAAAGCAAAAGGCACACAGGGTGAAGCTTCA
ACAACGAAATTTTCAACACAGTTTTAA

>g11439.t1 Gene=g11439 Length=368
MFKINTNTTNPPQMSTSQNFTLVPKTTRITDEYEISNNVLGLGINGKVVQCTRKSDNTKY
ALKVLHDNSKARREVELHWRVSGCRHIVNIVDVFENTYGGTKCLLVVMELMTGGELFNRI
QERQDGPFTEREAAQIMHEICIALKYLHDRNIAHRDLKPENLLYSSQDCDAIIKLTETHI
KDTLQTPCYTPYYAAPEILGPEKYDRSCDLWSLGVIMYILLCGFPPFYSNHGLAISPGMK
NRIRTGQYDFPLPEWQNVSNDAKDLIRGMLNVNPNERLTIDEVMKNNWIAQYTNVPQTPL
HTNRVLKEEEQQWPEVQEEMNRSLASMRVDYGLVLKNIDNSSNSLLNKRKKAKGTQGEAS
TTKFSTQF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g11439.t1 CDD cd14089 STKc_MAPKAPK 32 288 0.0
6 g11439.t1 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 16 111 6.8E-40
7 g11439.t1 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 112 293 1.1E-55
5 g11439.t1 Gene3D G3DSA:4.10.1170.10 MAP kinase activated protein kinase 2 297 362 3.5E-25
12 g11439.t1 MobiDBLite mobidb-lite consensus disorder prediction 346 368 -
2 g11439.t1 PANTHER PTHR24349 SERINE/THREONINE-PROTEIN KINASE 11 360 6.2E-152
3 g11439.t1 PANTHER PTHR24349:SF63 MAP KINASE-ACTIVATED PROTEIN KINASE 2 11 360 6.2E-152
1 g11439.t1 Pfam PF00069 Protein kinase domain 38 289 3.7E-59
10 g11439.t1 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 40 63 -
9 g11439.t1 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 152 164 -
13 g11439.t1 ProSiteProfiles PS50011 Protein kinase domain profile. 34 289 35.976
11 g11439.t1 SMART SM00220 serkin_6 34 289 3.0E-79
4 g11439.t1 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 22 329 9.84E-78

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values