Gene loci information

Transcript annotation

  • This transcript has been annotated as Acyl-CoA desaturase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g115 g115.t1 TTS g115.t1 996357 996357
chr_3 g115 g115.t1 isoform g115.t1 996419 998969
chr_3 g115 g115.t1 exon g115.t1.exon1 996419 996694
chr_3 g115 g115.t1 cds g115.t1.CDS1 996419 996694
chr_3 g115 g115.t1 exon g115.t1.exon2 996752 997010
chr_3 g115 g115.t1 cds g115.t1.CDS2 996752 997010
chr_3 g115 g115.t1 exon g115.t1.exon3 997069 997197
chr_3 g115 g115.t1 cds g115.t1.CDS3 997069 997197
chr_3 g115 g115.t1 exon g115.t1.exon4 997284 997360
chr_3 g115 g115.t1 cds g115.t1.CDS4 997284 997360
chr_3 g115 g115.t1 exon g115.t1.exon5 998503 998633
chr_3 g115 g115.t1 cds g115.t1.CDS5 998503 998633
chr_3 g115 g115.t1 exon g115.t1.exon6 998702 998969
chr_3 g115 g115.t1 cds g115.t1.CDS6 998702 998969
chr_3 g115 g115.t1 TSS g115.t1 NA NA

Sequences

>g115.t1 Gene=g115 Length=1140
ATGGCACCAGAAATAATGCAACCATCAAATCAGTCACAAAATGATAAAAATATTATACAT
CGTGTAAGCACAAATAAATTAGATGACAATCACAATTACAATCATGCAAATGGAAATGGA
AAAGTCGAAGCAGAAAATTCTCATGAAGTATTTAAGCCAAAAATACGATGGCCAGATTTT
ATAGTGCAATTATCATTACATATTGGATTTTTGTATGGTCTCTATTACTTAATCGCACTA
AAAGCAAAATTCTACACATACATTTGGTTCTTTGTCCTCATTTATGTAACAGGTTTAGGT
ATTACAGCCGGTGCGCATCGTCTATGGAGTCATCGTTCATACAAAGCAAAACTTCCATTG
CGTGTCCTTCTTTTGATATTTTTCACAATTGCTGGACAGAGAGACGCATATACATGGGCA
CACGATCATCGCATTCATCATAAATATTCTGAAACTGATTCTGATCCACATAATGCAAAA
CGTGGATTCTTTTTTGCTCATGTCGGCTGGTTATTTTTAACACCACATCCTGATGTCGTT
GAAAAAAGAAAGTCAATTGATATGCGAGATTTGCAAGCAGATCCTTTACTTATGTGGCAG
AAAAAATATTATCCACCATTATTTGCTCTTCTAGTCATATTTTTTCCTACAATCGTGCCT
TATTATTTTTGGAATGAAGACTTGTGGATTTCTTTTTGGACAGCTTTTGTTTGTCGTTTT
GTAACTGTCTTAAATATTGCATTTTTTGTGAATAGCGTTGCACACATGTATGGAACAAAA
CCATATGATAAGAAAATTATGCCAGTAGAAAATTTAGCAGTTGCAATAACAGCAATGGGT
GAAGGTTGGCACAATTATCATCATGTCTTTCCCTATGATTATAAAACTGGTGAATTTGGT
GGATGGCAAGGATATAAATTTAATATCACAACAGCTCACATTGACTTTTTTGCTAAAATT
GGCTGGGCTTATGATCGAAAATATGCAAGTGCTGAAATGATTGCAAGAAAAGTTGCAAAA
TCAGGCGATGGAAGTCATTTTTTATCACATGAAGAAGCACACAAAACTTCAATTTATGGT
TATGGTGATAAAGATCTTGAAATTGAAGATGCTAAAGCATTAGAAAATTTAAAAGAATGA

>g115.t1 Gene=g115 Length=379
MAPEIMQPSNQSQNDKNIIHRVSTNKLDDNHNYNHANGNGKVEAENSHEVFKPKIRWPDF
IVQLSLHIGFLYGLYYLIALKAKFYTYIWFFVLIYVTGLGITAGAHRLWSHRSYKAKLPL
RVLLLIFFTIAGQRDAYTWAHDHRIHHKYSETDSDPHNAKRGFFFAHVGWLFLTPHPDVV
EKRKSIDMRDLQADPLLMWQKKYYPPLFALLVIFFPTIVPYYFWNEDLWISFWTAFVCRF
VTVLNIAFFVNSVAHMYGTKPYDKKIMPVENLAVAITAMGEGWHNYHHVFPYDYKTGEFG
GWQGYKFNITTAHIDFFAKIGWAYDRKYASAEMIARKVAKSGDGSHFLSHEEAHKTSIYG
YGDKDLEIEDAKALENLKE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
24 g115.t1 CDD cd03505 Delta9-FADS-like 86 327 1.9921E-70
14 g115.t1 Coils Coil Coil 368 379 -
2 g115.t1 PANTHER PTHR11351 ACYL-COA DESATURASE 13 377 2.3E-182
3 g115.t1 PANTHER PTHR11351:SF61 RH14937P 13 377 2.3E-182
9 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 57 77 2.0E-51
7 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 106 126 2.0E-51
8 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 143 172 2.0E-51
4 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 204 222 2.0E-51
6 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 237 258 2.0E-51
5 g115.t1 PRINTS PR00075 Fatty acid desaturase family 1 signature 280 294 2.0E-51
1 g115.t1 Pfam PF00487 Fatty acid desaturase 89 291 6.0E-16
15 g115.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 59 -
23 g115.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 60 78 -
19 g115.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 79 83 -
21 g115.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 84 105 -
16 g115.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 106 206 -
20 g115.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 207 224 -
18 g115.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 225 229 -
22 g115.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 230 250 -
17 g115.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 251 379 -
13 g115.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 60 79 -
12 g115.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 84 106 -
10 g115.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 207 224 -
11 g115.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 228 250 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055114 NA NA
GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water MF
GO:0006629 lipid metabolic process BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values