Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable multidrug resistance-associated protein lethal(2)03659.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g11523 g11523.t1 TSS g11523.t1 16788486 16788486
chr_1 g11523 g11523.t1 isoform g11523.t1 16789143 16797471
chr_1 g11523 g11523.t1 exon g11523.t1.exon1 16789143 16789241
chr_1 g11523 g11523.t1 cds g11523.t1.CDS1 16789143 16789241
chr_1 g11523 g11523.t1 exon g11523.t1.exon2 16789325 16789410
chr_1 g11523 g11523.t1 cds g11523.t1.CDS2 16789325 16789410
chr_1 g11523 g11523.t1 exon g11523.t1.exon3 16791704 16792012
chr_1 g11523 g11523.t1 cds g11523.t1.CDS3 16791704 16792012
chr_1 g11523 g11523.t1 exon g11523.t1.exon4 16792323 16792594
chr_1 g11523 g11523.t1 cds g11523.t1.CDS4 16792323 16792594
chr_1 g11523 g11523.t1 exon g11523.t1.exon5 16792736 16792859
chr_1 g11523 g11523.t1 cds g11523.t1.CDS5 16792736 16792859
chr_1 g11523 g11523.t1 exon g11523.t1.exon6 16793647 16794001
chr_1 g11523 g11523.t1 cds g11523.t1.CDS6 16793647 16794001
chr_1 g11523 g11523.t1 exon g11523.t1.exon7 16794098 16795567
chr_1 g11523 g11523.t1 cds g11523.t1.CDS7 16794098 16795567
chr_1 g11523 g11523.t1 exon g11523.t1.exon8 16795622 16795778
chr_1 g11523 g11523.t1 cds g11523.t1.CDS8 16795622 16795778
chr_1 g11523 g11523.t1 exon g11523.t1.exon9 16795908 16796060
chr_1 g11523 g11523.t1 cds g11523.t1.CDS9 16795908 16796060
chr_1 g11523 g11523.t1 exon g11523.t1.exon10 16796152 16796424
chr_1 g11523 g11523.t1 cds g11523.t1.CDS10 16796152 16796424
chr_1 g11523 g11523.t1 exon g11523.t1.exon11 16796505 16796616
chr_1 g11523 g11523.t1 cds g11523.t1.CDS11 16796505 16796616
chr_1 g11523 g11523.t1 exon g11523.t1.exon12 16796702 16797024
chr_1 g11523 g11523.t1 cds g11523.t1.CDS12 16796702 16797024
chr_1 g11523 g11523.t1 exon g11523.t1.exon13 16797098 16797471
chr_1 g11523 g11523.t1 cds g11523.t1.CDS13 16797098 16797471
chr_1 g11523 g11523.t1 TTS g11523.t1 16797551 16797551

Sequences

>g11523.t1 Gene=g11523 Length=4107
ATGGACTATTCAAAAAAGCCAACGAAAAAAAATCCAAGAAATGGAGCGTTGGCTTTATCA
CAATTATTTTTTCTCTGGACAATACCGACAATGCTTCGAGGATGCTTTTACGGTCTGAAT
CGTGAGGACTTAACAAAATGTTTGAAGAAAGATCGTTCACAAGATCTGGGTGAAAAGCTT
GAAGTAGAGTGGGAAAAAGAGTTGGAACAAGCGAGGAAAAAGAACCGAAAACCACAATTT
AGGAATGCGCTTTTTCACACATTCTGGAGAAGTTGTGTTGTAGATGGCATGTTAGTGTTC
ATATTCGTTCTCTTGAAATCAATTGGTCCAGTATTTTTTTCTCAACTTTTGCTGCAATTT
CAAGAACCACAAAATGCAATTATAAATGACTCGTCATCAAATTTTAATGAAACTGTCGAA
TTATCAAATACCACAACGCCGCTACCATCAACATTCAATTCAAATGACTTTCTTCAATAT
ATGGAGTATATTTGGACTGACGTATATTGGATGGCGACAATAGTTATATTAATTACCTTT
ATCTCCTGCATCATTGGTCATCACATGGACTTGCGACAGAGAATGGTCGGTGCTCGAATG
CGCATTGCTTGTTCAGCAGCAATTTATCGTAAATCATTGCGAATGTCAAAGAGAGCCGTT
ACGCAAACATCTGCTGGAAACATTGTAAATCTTCTATCGAATGATGTGAGCCGTTTAGAT
TATGGGTTCATTTTTGTCCATTTCATCTGGATATTGCCATTTCAAGCCAGCTTAATTTGT
TATTTAATATGGAGAGAAGTGAAATACGCTGCAATAGTTGGTGTGCTTGGATTGATAATA
AAAACAGTTCCAATTCAAACTGGATTATCAAAAATTTCCTCGATTCTAAGAATGCGAATA
GCAATAAGAACCGACAATCGTGTATCCATTATGAATGAACTCATTCAGGGAATACAAGTA
ATCAAAATGTATGCATGGGAGACGCCTTTCAGAAATGTTGTTAAGCTGGCACGAAATAAA
GAGGTGGATCAAATTCAGAAGGCGCAATATATAAGAGGCATATATTTGTCAACTATGGTT
TTTACTGAACGTTCTACACTTTTTTTGGCCATTCTCGCATGCATATTGGAAGGACGTGCG
ATCACAGCTGATTTAGTGTTTTCGATGGCAACATTTTTTAACGTCCTTCAATTGACGGCA
GCAATATTTTATCCACTAGCAGTATCGTTAGGAGCTGAAGCATTGGTCTCTATAAAAAGA
ATTCAAGATTTTTTGAGTTTAGAGGAGCAAGATTCAGCAATGCCTGGCTTACATCAAAAT
AAAATAGAAGTAATTGAATCAGAAACGAATGTCATCGAGATGATTAATGTCGACGCTACA
TGGACAAATGATTATCGTTGTAAAACTTTGTCGAATCTCAATATTAAAGTAAAAGCAGGA
AAACTCTGTGCAATCATTGGTCATGTCGGAAGTGGAAAAAGCTCAATTTTTCAGCTTCTA
CTTGGCGAACTTTCAATTTACTCAGGTGACGTTGTTATAAATGGTGAATTGTCATATGCC
TCTCAGGATCCATGGTTATTTTCTGCATCAATAAAGAACAACATTCTTTTTGGATTACAA
TACAATAAGACTCGTTATCAGGACGTTATAAAGCATTGTGCACTTGCTACAGATTTCCAA
CAGTTACAATATGGTGATAAAACTTTCGTTGGTGAACGAGGGTGCGCACTTTCGGGAGGT
CAAAGAGCACGCGTGAGTCTCGCTCGTGCAGTTTATAGAAATGCATCAATTTATCTCCTC
GATGATCCGCTATCTGCAGTAGACGCTCAAGTTGGACAACATATTTTCAATGAATGTATT
GGTCCACATGGTTATCTTGCACGACAGAGGGCAACGAGAATTTTGATTACACATCAGGTG
CATTTTTTGAAGGAAGCAGATTGGATTGTTGTTATGGATCAAGGTAGAATTATTAGGCAA
GGTACATGGAACGATGTAATGGATATTGACATTTCACAATATGTTCTTTCACTCGAAACA
AAAGAACAAAGCAGTGATGATGATTTGGATGAAAAAGAATCTAAAATGAATTTAACCAAT
GACATCGACGATATTCCATATATTGATGACGTGCCAGAGAGCAATAATGGTTACATAAAG
CTTAGAGCAACAGATTTAAAGAAATCACTGTCACGTGCTTCAAGTGCCAATAGTGTTGAG
GAAAATCTTGAGCTTATCGAGCAAACTGAAGCACGCGATGAAGAGAAAAGCACCCAGAAC
ATTCACTTTATAAAAGTTTTTTATGAATATTTTCGTGCTGGGGCAAGTCCTTTAGTGTTG
CTCTCAATTGTCATTTATTTACTCTTCTCACAAGCAGTGACTTCCGGAAGCGATTTTTTC
CTTACATTTTGGACAAATCAAGAGCTATTACGCTTACATGGTGGGAAGACAGTCATCGAA
ACAACAGATGGCCTTTATATTTATGGATTTTTAATACTTGCTGTCATCTTTGTTACCATG
TCGCGCGGCTTCATTTTCTTTGCCGTTTGTATGCGCGCATCAAAGAAGCTTCATGATAAG
AGCTTTCTTTGTTTGCTTCACAGCCCCATGCGGTTCTTCGATTTGAATCCCGTTGGGCGA
ATCTTGAATAGATTTAGTAAAGACATGGGTGCGGTAGATGAGCTCTTGCCAAAAGCAATT
ATTGAAGCAATTCAGAATTTGCTTGTGATGCTTGGTGTATTAGTTTTAATTGGAATTACT
ACAACTGGATATGTACTTGTGGTTGCGTTAATAGGTGCAATTATTCTCTATGTATTAATT
CTGCGTCTTTATTTGCAACCAGCTAGAGATTTGAAAAGACTAGAGGGAATTACCAAGAGT
CCCGTGTTCAGTCAAATGTCGGCTACTTTAATTGGTATATCGACCATTCGAGCGTCCAAT
ATGAATTCTCAATTAACTCTTGAATTTGATAAACTCCAGAATGTTCATTCAAGTGTATGG
CAGACACTCTGGAGCATCAATACAGCTTTGGGACTTTGGTTAGATTGTGTATCATGTACA
TTTGTGGCATGTATCTGCTTTAGTTTCATCATGATGAGTCAGGAGAATACAAACAGCTCA
AATGTTGGTCTTGCTATTTCGCAAGCCTTGATTTTGACTGGAATGGTACAGTATGGAATC
AAGCAAATGATGGAGTCATTTCAACTTTTTACTAACACTGAACGTGTTCTTGAATACACG
AGATTAGATCAGGAACCTTTAATTTCTAGAAAGCCCACACGAGATTGGCCATTTAAAGGC
TTGATTGAAATCAAAAACATGTCGCTCTATTATGATATCCATTCATCTCCCGCTTTAAAG
AATTTAAATTTAGTTATTGAACCTGGATCAAAAATAGGAATAGTTGGGCGAACGGGTGCG
GGAAAGAGCTCAATCATAGCTGCACTATTCCGTTTGTCATATATTGATGGTCGTATATTG
ATTGATGGCATCGACACGAGTGAAATAAATCTCGAAAGCTTACGTTCAAAAATTTCAATT
ATTCCACAAAATCCAGTTTTATTCAGCGCTACAATACGTTACAACTTAGATCCATTCTCG
TTATTTTCAGACGAGGAAATATGGCGCGCATTAGAGCTAGTTGAGCTAAAGTCAGCCATA
AATGGATTGCAATTTATGGTCACTGAGGGTGGAAGCAATTTTTCAGTTGGTCAAAGGCAA
TTATTGTGTTTAGCGCGAGCGACATTGAGAAAAAACAAAATCCTTCTCCTTGATGAAGCC
ACTGCCAACATAGATCAACAGACGGATGCATTAATTCAAAAAACCATTCGAGAGAAGTTT
TCGCATTGTACTGTTCTTACAATTGCTCATCGGTTACATACAGTAATGGATTCTGATAAA
ATACTTGTTATGGATGATGGTTATGCAAAAGAATTTGATATTCCATACAAATTACTGCAA
CAACCATTTGGTGTTTTTAAAGATCTTGTTAATAATTTAGACGATATTGATAGACAAAAT
TTAATTGAAATTGCAAAACGAAAATATGAAGAAATTTTTGATCAAGAGGAGCAGAGGGAT
AGTCTTTGGAAATTTCGAAATGATTGA

>g11523.t1 Gene=g11523 Length=1368
MDYSKKPTKKNPRNGALALSQLFFLWTIPTMLRGCFYGLNREDLTKCLKKDRSQDLGEKL
EVEWEKELEQARKKNRKPQFRNALFHTFWRSCVVDGMLVFIFVLLKSIGPVFFSQLLLQF
QEPQNAIINDSSSNFNETVELSNTTTPLPSTFNSNDFLQYMEYIWTDVYWMATIVILITF
ISCIIGHHMDLRQRMVGARMRIACSAAIYRKSLRMSKRAVTQTSAGNIVNLLSNDVSRLD
YGFIFVHFIWILPFQASLICYLIWREVKYAAIVGVLGLIIKTVPIQTGLSKISSILRMRI
AIRTDNRVSIMNELIQGIQVIKMYAWETPFRNVVKLARNKEVDQIQKAQYIRGIYLSTMV
FTERSTLFLAILACILEGRAITADLVFSMATFFNVLQLTAAIFYPLAVSLGAEALVSIKR
IQDFLSLEEQDSAMPGLHQNKIEVIESETNVIEMINVDATWTNDYRCKTLSNLNIKVKAG
KLCAIIGHVGSGKSSIFQLLLGELSIYSGDVVINGELSYASQDPWLFSASIKNNILFGLQ
YNKTRYQDVIKHCALATDFQQLQYGDKTFVGERGCALSGGQRARVSLARAVYRNASIYLL
DDPLSAVDAQVGQHIFNECIGPHGYLARQRATRILITHQVHFLKEADWIVVMDQGRIIRQ
GTWNDVMDIDISQYVLSLETKEQSSDDDLDEKESKMNLTNDIDDIPYIDDVPESNNGYIK
LRATDLKKSLSRASSANSVEENLELIEQTEARDEEKSTQNIHFIKVFYEYFRAGASPLVL
LSIVIYLLFSQAVTSGSDFFLTFWTNQELLRLHGGKTVIETTDGLYIYGFLILAVIFVTM
SRGFIFFAVCMRASKKLHDKSFLCLLHSPMRFFDLNPVGRILNRFSKDMGAVDELLPKAI
IEAIQNLLVMLGVLVLIGITTTGYVLVVALIGAIILYVLILRLYLQPARDLKRLEGITKS
PVFSQMSATLIGISTIRASNMNSQLTLEFDKLQNVHSSVWQTLWSINTALGLWLDCVSCT
FVACICFSFIMMSQENTNSSNVGLAISQALILTGMVQYGIKQMMESFQLFTNTERVLEYT
RLDQEPLISRKPTRDWPFKGLIEIKNMSLYYDIHSSPALKNLNLVIEPGSKIGIVGRTGA
GKSSIIAALFRLSYIDGRILIDGIDTSEINLESLRSKISIIPQNPVLFSATIRYNLDPFS
LFSDEEIWRALELVELKSAINGLQFMVTEGGSNFSVGQRQLLCLARATLRKNKILLLDEA
TANIDQQTDALIQKTIREKFSHCTVLTIAHRLHTVMDSDKILVMDDGYAKEFDIPYKLLQ
QPFGVFKDLVNNLDDIDRQNLIEIAKRKYEEIFDQEEQRDSLWKFRND

Protein features from InterProScan

Transcript Database ID Name Start End E.value
46 g11523.t1 CDD cd18579 ABC_6TM_ABCC_D1 155 421 4.02287E-85
48 g11523.t1 CDD cd03250 ABCC_MRP_domain1 452 656 1.56895E-96
45 g11523.t1 CDD cd18580 ABC_6TM_ABCC_D2 780 1080 1.30205E-79
47 g11523.t1 CDD cd03244 ABCC_MRP_domain2 1100 1315 2.49047E-119
17 g11523.t1 Coils Coil Coil 736 756 -
16 g11523.t1 Gene3D G3DSA:1.20.1560.10 - 145 424 1.7E-10
13 g11523.t1 Gene3D G3DSA:3.40.50.300 - 425 704 4.1E-72
15 g11523.t1 Gene3D G3DSA:1.20.1560.10 - 744 1086 2.8E-30
14 g11523.t1 Gene3D G3DSA:3.40.50.300 - 1087 1331 1.4E-77
6 g11523.t1 PANTHER PTHR24223:SF334 FI20190P1 1 132 0.0
8 g11523.t1 PANTHER PTHR24223 ATP-BINDING CASSETTE SUB-FAMILY C 1 132 0.0
5 g11523.t1 PANTHER PTHR24223:SF334 FI20190P1 156 1334 0.0
7 g11523.t1 PANTHER PTHR24223 ATP-BINDING CASSETTE SUB-FAMILY C 156 1334 0.0
1 g11523.t1 Pfam PF00664 ABC transporter transmembrane region 146 397 5.2E-19
3 g11523.t1 Pfam PF00005 ABC transporter 470 604 3.8E-21
2 g11523.t1 Pfam PF00664 ABC transporter transmembrane region 779 1054 1.9E-28
4 g11523.t1 Pfam PF00005 ABC transporter 1119 1262 3.0E-30
28 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 19 -
37 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 20 39 -
20 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 40 82 -
42 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 83 105 -
27 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 106 167 -
39 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 168 191 -
18 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 192 240 -
33 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 241 264 -
25 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 265 269 -
41 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 270 289 -
23 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 290 366 -
35 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 367 389 -
29 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 390 394 -
34 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 395 416 -
24 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 417 777 -
43 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 778 805 -
31 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 806 824 -
40 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 825 850 -
21 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 851 898 -
38 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 899 919 -
30 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 920 924 -
36 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 925 945 -
19 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 946 1009 -
44 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 1010 1030 -
26 g11523.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1031 1041 -
32 g11523.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 1042 1060 -
22 g11523.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1061 1368 -
64 g11523.t1 ProSitePatterns PS00211 ABC transporters family signature. 577 591 -
63 g11523.t1 ProSitePatterns PS00211 ABC transporters family signature. 1234 1248 -
66 g11523.t1 ProSiteProfiles PS50929 ABC transporter integral membrane type-1 fused domain profile. 98 412 24.328
67 g11523.t1 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile. 452 679 22.136
65 g11523.t1 ProSiteProfiles PS50929 ABC transporter integral membrane type-1 fused domain profile. 779 1072 28.148
68 g11523.t1 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile. 1102 1331 16.713
61 g11523.t1 SMART SM00382 AAA_5 479 662 1.5E-8
62 g11523.t1 SMART SM00382 AAA_5 1128 1318 7.3E-11
10 g11523.t1 SUPERFAMILY SSF90123 ABC transporter transmembrane region 80 589 3.4E-24
12 g11523.t1 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 447 669 4.53E-55
9 g11523.t1 SUPERFAMILY SSF90123 ABC transporter transmembrane region 784 1085 8.5E-42
11 g11523.t1 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 1093 1330 2.09E-66
51 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 15 37 -
54 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 82 104 -
59 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 163 185 -
52 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 243 265 -
56 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 270 292 -
58 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 353 375 -
60 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 390 412 -
49 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 773 795 -
50 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 824 846 -
57 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 907 941 -
53 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 1009 1031 -
55 g11523.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 1038 1060 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055085 transmembrane transport BP
GO:0005524 ATP binding MF
GO:0042626 ATPase-coupled transmembrane transporter activity MF
GO:0016021 integral component of membrane CC
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values