Gene loci information

Transcript annotation

  • This transcript has been annotated as Mitogen-activated protein kinase kinase kinase kinase 3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g12126 g12126.t8 isoform g12126.t8 21184066 21185582
chr_1 g12126 g12126.t8 exon g12126.t8.exon1 21184066 21184435
chr_1 g12126 g12126.t8 cds g12126.t8.CDS1 21184067 21184435
chr_1 g12126 g12126.t8 exon g12126.t8.exon2 21184495 21184526
chr_1 g12126 g12126.t8 cds g12126.t8.CDS2 21184495 21184526
chr_1 g12126 g12126.t8 exon g12126.t8.exon3 21184595 21184688
chr_1 g12126 g12126.t8 cds g12126.t8.CDS3 21184595 21184688
chr_1 g12126 g12126.t8 exon g12126.t8.exon4 21184795 21185089
chr_1 g12126 g12126.t8 cds g12126.t8.CDS4 21184795 21185089
chr_1 g12126 g12126.t8 exon g12126.t8.exon5 21185272 21185399
chr_1 g12126 g12126.t8 cds g12126.t8.CDS5 21185272 21185399
chr_1 g12126 g12126.t8 exon g12126.t8.exon6 21185496 21185582
chr_1 g12126 g12126.t8 cds g12126.t8.CDS6 21185496 21185582
chr_1 g12126 g12126.t8 TSS g12126.t8 21185641 21185641
chr_1 g12126 g12126.t8 TTS g12126.t8 NA NA

Sequences

>g12126.t8 Gene=g12126 Length=1006
ATGCAGCCGATATACAAAAGTAATCCGCATGAAGATTACGAGTTGTTGCAGAAAATAGGC
TCAGGCACTTATGGGAGTGTGTATAAAGGAAGGAATGTAAAAACGGGCGAATATGCAGCT
ATTAAAGTCATAAAAATAGAAACGGGCGATGACTTTCAACAGATCGAACAAGAAATCACC
ATGCTCAAGGGCTGCAATCATAAAAACATCATAAGTTACTATGGCTCATATCTTCGTCGA
GATAAATTGTGGATATGCATGGAATTGTGTGCGTACAGCTTGCAAGATATTTATCAAGTC
ATTGGACCACTGACAGAACAGCAAATAGCATACGTGTGTCGAGAAACGTTACTTGGTTTA
CAATATTTGCATAATCGAGGAAAGATGCATAGAGATATCAAGGGAGCCAATATTTTGTTG
ACTGACAATGGTGATGTAAAATTAGCTGATTTTGGAGTGTCTGCACAAATCACTGCAACA
ATTAATAAGCGGAAAAGTTTTATTGGAACTCCATATTGGATGAGTCCAGAAGTTGCTGCA
GTTGAAAGAAAAGGAGGATATAATCATTTGTGTGATATTTGGGCGGTTGGGATTACTGCT
ATTGAACTTGCTGAGCTTCAACCCCCGATGTTTGAGATGCATCCAATGAGAGTTTTATTT
CTTATGTCTAAAAGCAACTTTAAACCTCCTACGTTAAAGGAGAAAGAGCTGTGGTCACCT
ATATTTCATTCCTTTGTTAAAATCGCACTTACGAAAAATCCGAAAAAGCGTCCAGATTCT
GAGCGATTACTTCAGCATCCGTTTGTTAATGGTGGTGGTGCTGGAACGGGCGAAATGTCG
GTTCGTTTGATGCGAGAACTTTTGCACAAATATCAAAATCCTCACAATTACAATGATCAT
GAGATTGACGATGAAGGTGCTGTAGGCATTTCTTCTGTTCCAAAACGCATTCCAAGTAAA
ATCGGGACTGTAGAAAGAGTTCATCATATGCATGGAGAAAGCTCAA

>g12126.t8 Gene=g12126 Length=335
MQPIYKSNPHEDYELLQKIGSGTYGSVYKGRNVKTGEYAAIKVIKIETGDDFQQIEQEIT
MLKGCNHKNIISYYGSYLRRDKLWICMELCAYSLQDIYQVIGPLTEQQIAYVCRETLLGL
QYLHNRGKMHRDIKGANILLTDNGDVKLADFGVSAQITATINKRKSFIGTPYWMSPEVAA
VERKGGYNHLCDIWAVGITAIELAELQPPMFEMHPMRVLFLMSKSNFKPPTLKEKELWSP
IFHSFVKIALTKNPKKRPDSERLLQHPFVNGGGAGTGEMSVRLMRELLHKYQNPHNYNDH
EIDDEGAVGISSVPKRIPSKIGTVERVHHMHGESS

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g12126.t8 CDD cd06613 STKc_MAP4K3_like 12 269 0.0
5 g12126.t8 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 9 315 3.1E-109
2 g12126.t8 PANTHER PTHR48012:SF18 HAPPYHOUR, ISOFORM A 4 320 4.9E-175
3 g12126.t8 PANTHER PTHR48012 STERILE20-LIKE KINASE, ISOFORM B-RELATED 4 320 4.9E-175
1 g12126.t8 Pfam PF00069 Protein kinase domain 13 269 3.7E-61
7 g12126.t8 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 19 42 -
9 g12126.t8 ProSiteProfiles PS50011 Protein kinase domain profile. 13 269 46.251
8 g12126.t8 SMART SM00220 serkin_6 13 269 4.0E-91
4 g12126.t8 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 6 271 2.56E-84

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values