Chromosome | Gene | Transcript | Category | ID | Start | End |
---|---|---|---|---|---|---|
chr_1 | g12129 | g12129.t1 | isoform | g12129.t1 | 21203400 | 21212391 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon1 | 21203400 | 21203627 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS1 | 21203400 | 21203627 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon2 | 21205602 | 21205643 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS2 | 21205602 | 21205643 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon3 | 21205713 | 21205855 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS3 | 21205713 | 21205855 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon4 | 21205920 | 21207347 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS4 | 21205920 | 21207347 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon5 | 21207477 | 21211337 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS5 | 21207477 | 21211337 |
chr_1 | g12129 | g12129.t1 | exon | g12129.t1.exon6 | 21211425 | 21212391 |
chr_1 | g12129 | g12129.t1 | cds | g12129.t1.CDS6 | 21211425 | 21212391 |
chr_1 | g12129 | g12129.t1 | TSS | g12129.t1 | NA | NA |
chr_1 | g12129 | g12129.t1 | TTS | g12129.t1 | NA | NA |
>g12129.t1 Gene=g12129 Length=6669
ATGGACAAATATGGTGAAAGTAAATCAAATAGAAATCGATGGAAGGATGAACATCGTCAT
CCAAAATTGTATGATGAACGACTGGACACTCCAGGACAACAACAAAATGGAAAAATTACT
ACTGCCAGTTCCTCATCTTCTTCTTCAAAGACACAAAGTAGTAATAGCAGCAGTAATAAA
CTTTTGAAAACTGACATAGTCAATCAACTAGCTCAAAATCCAAATCTTCCTGAGGATGTT
CGCATTTCAAAGTTGTTGCGACGTTTAGAGACGGAAACTACTGTAACTGGAACAATTGAA
ATTTGTGGAAAACTTAAAACAGTCGTTCAAGACCAAGCAAATGCCAATTACATCAAGCGA
TCGTTTGACATGCTCACCAATTCATTAATTTCGGTTATGAAGGAATGTCACACAGGTGAA
GCAGTCAATCATGTTGCGGAAGTATATGGAATGATGGGTTTTGTGATGAGAAATGATTTT
CCTCTTTATAAATCATGGATTTGCAAGACTTACAAGAATGTTAAAACACTTCGTGTCGCA
ATGATGAAAGCTTTAGAAAAAACTCTCACAATGGACTCAAATGAGCTACGATTGAGTGAT
CAAATAGGAAGATTGATGGAATTATTGAGAGATTTTCTTGAACAGACTGATGATTATCGA
GTTTTTATGGCTATCACAAATGTTTTAATTGTCATTGCTAATAATTATAATATGAGATAT
TTTAAGTCACATTTTACAAATGTGGTCGATATTGTAATTGGTTGGATGATGGAAAATCAA
TTGACAAAAGTGAAAGTTCATTGTGCAGTTGTTTTACAAAGTTTGGGACAATTTTGGAAA
AATGATTCACAATTTTCACTTGATCTTATTGGTCAACTTTTAGAAGATATCGATGGATGC
ATTGAAAAAATGGAAGACAGCAACGAAGATAAAGTTAAAAATTTTAAAGATTTTGGTGCA
TTTGTTGGTGTCTTCAATGCGATAATAAAATCAGTTTATGTTTCGCCCGAAGCATTAATT
TATTTTATGGGAAAATCGACAATGGAAGGGTGCAAAGAAAAGATTTTTCGAGTAGCACAG
TTTGCCTTAAATAATGAAAACTGCATCAAAGAGACAGTTTTACCAGTGAATGAATACATA
TTAATTGTTCTTAATTGCCGCAAGTGTGAAATTGGAATAGAAATGAACTCAATTCTGACA
TTGATTGAAATCGAAATGGAAAATTTAACAAAATTTACAGAAAGTGAATTGGCTAGTTTC
CTTTGTGTCGTTAATGAATTTATCAAAGAGTTCAAAAGTGAACTTCCTGTGCAATTTATT
GAAAAGCTTTTCAATGTTGATAGTGTGCTGATTCAAAGTGTCAGTTTCAATAACGATAAA
CGAGTGAAAAGCGGATTAATTAAAATTTATCATGAAGTTCTTGCATTGAAAAATGTTCCA
TTATTACAAGCTGCATATAGATGTATAATTGAACAATTTGGCGTATGCCTTAAGCGAATT
CCACAATTAAAGGAAATTAAATGGATTTCCGATGTGGTGTCTAATTTGGTTAATAATGAA
ATAACTAATGAGAAAACAATTCTGAGAGCACAAGTTGCATTGAATTTTAATTTAACTGCT
CTTTCAAAATTGGCCACAACGCAAAATTCAATCATTGCCATGTATTCGCTTGTTCCGAGT
ATTTTAGAAGTATTGATAAATTTGCAAATTTGGCGATCTGAGTGGAATGAGTTTGAATTG
ACACAATATGCAATTTTGCTGGTAATTTCGGAGCATTGTCTGAAAAATAACAATTTTATT
TCATCAAGTGCACTGCTAATCAACAAAACTTCAACAATAACTACATCTTCATGGCTTTCT
GATTCGTCGCCAACGGAATCACCTGTTTCGCAACACTTCAAACTTATTCTTGAATTTATT
GATAAAATGCTCAAGTCTTCACCATCATTAAAACAATTCAAATTGATTCTTGATTGGCTT
GATAAAATCATTCAGCAAACATGTCAATATTCTGAACAATTAAAAGAAAATTCAAGTTTT
GTATCAATTCTGAAGCGAATAAATCAAATCGCAAATAATTATAAGAATGATGAAGTAATG
CTAAAGGTCGCAACATGTAATGATTCACTCTATTCCTTTGAGAGCATTCACAATGAAATT
TACACCGCTATGTCTGAAGCAGGTGTTGTACAAATGTGCTCAACAAATCCAGAAATAAGA
CGCCGTTATTCCTTCATTCTCTCGCGTATTCCGTTGAAATTTACGCTCGAACAAGCCAAG
TCACCATCGGGCATAAATCAAGAGACTATTAATAAAATCACCGAGATGGAAAACTGGCAT
CTATCACTCGGCAGCATTCATGGAGGAGAATTGCGCGCTCAATATTTCCAAAAATTCATA
AATCATATAACTTATTCGCCAAATGCCGCTAATATTGATCAGTTTATTTTGCATGCATTC
AGAAATTGTTGGTTTAATGGGACCGAAATGGCTGAAGAATATAAGCGAGTAACATTAAAG
GATGTTCGAACATTGTACTCTTGGATGCAATGGGAAGCAGCTCGCTTTTGTGTCAACAAT
AAATTACGAACGCCGCTGGGCAAGGCACAGGACACTTTCATTAAAATTGAAGGGATAATT
CGAGAGCACGCTCGTATTCTTACGCTCAAGGACAAGTCGAAACTGCGTGGTTATGAGCAC
GTCTTGGCAAATCAACGCAACGTGCGAATTCTTTTAGGTTTTATGGAAAGTTTAGAGAAA
GCTATTTATAATGCGGCAGAGGGTAGTGCTTTTGCAATACCACCTCCAGAAAAGCCAGCA
AGAACATTTTTCCGACTGAATGCGTCAACTTGTAATGAATGGTTTAATCGCAATCGACTT
GCAATTCATCAGTTAGCACTTCATGCACTAGAACTTGAGATGGTAATTAGATGTTCACAA
AGCATTTTAAAGGAAATGTGTGCTGCCGGAAAAATAAATGAGACTTATTTTGATCAAATA
TTAATGTCATTAACATGGGCCATGTTACGAAATTTCGAAAGCGACAGTCTATGTGGTTTG
TATGTTTGGGTAAAAAATGTCTCTGGTAGAAAATTACATTGGATTAAAATGGCAGCGGAA
CAAGCTGCAGGACATCGTGAAGTAGCAGCCGATGGTTATACAAAAGTTCTAAAAGATGAA
AAACTCGAACCGAAAATTTATGATTTCATTGCGGATCAAAGAAGATGTTGCTTGTTGTTT
ACAGGAAATATGGAGGAGTTGTATAATGTTTTGGTTGAAGAAGAAGAGAGAAATTATAAG
CCAACAAATATTCCAATATTAAAAATCACAAAGGAACAGGTGGCCAGTATCAAGAAATAT
GACAAGACGAAGGACTTTGATGCACTTAATGATTTATCACTGTGGGAGACGCTTGAGGAT
GAAGGAAATGTTTCAAACAACTTTTCAGTGCATAATTTACTCTCATTAACTGAGAACACG
CTCTCATGTGCCATATTAGACAAAGAGAATAGCAATCGAGATAAAGAGAAGACAAGCTGG
GAAATTTTGCACACTTTATTGCAAGAGAGCATTAGAACAAGTTCACAAGAATATTTAATT
GGACTAAATGTATTGAATCACATAGCACACAAATACAAAATAGTCATCAATAATGAAGGC
AGTGTCAATATTGAGTCACTCGAAATTGAGAAGAAATATGGTTCGTTGACAATGTTTTTT
GTTTCAATTTATGGTGAATTTCTCGACGCATGCAGCGAGAAGGGTGAACAACAAAATATC
AATCTGAAATTAGATTTGGTCGCATCTGCACGCAAAGAATTAAATTTTCGACAATGCACT
CGTGAGCTATCATCAGTTTATCGAAGTGTAGAATATACAGAGCACATAGGTCTCCCATTT
GAGCACGAATTGTCATCGATAAAAGAATTTTTCATGTCACCACGCAATGTCAAAGATAGT
AAAATTTGGTCTGAAAATGTATCACGTGCGATTTATGAGCATTGCAAAATGCTTTATGTT
GCGAAAAATCAACATTTTGATGCTATACAATTTGCTTCAACCGCAGCACTCGGTATTAAA
AATAGACTAAACATAACTGATGGCAATGTTTCTGAAAATTTAAATCAACAGTGCGTTAAA
TTTTACACGAAAATTTCAGAATGGATCCAAAATGAGCCCGAGGAATCACTGTTTTCACAC
GATGGTAACTCGCTAAATTCACTCGTGCAGTCAATAAATGATGAAGAATATATAGACGAA
CAAATGCCATTAGTTGATTCTGCGGTCGGAAAACTTCTTCAAAGTGGTGTAAAACAATGT
CCTGAAATGCCTAAAGCTTGGTCAAGTCTTGCAAATTGGTGCTATAAATGGGGCAGAAAA
ATGATTGAATCAAAAACCGACAGTCAAGGTCTTCGAGCCATCGACTCAAGTGCAATTGTA
GATTTAATTCCTGAAGCGCAACCGGAAGATGTTGAGAGTATCTTGAAAGTTCTAAACGAG
CAGCAAATTATGGTCGAGGACGAAGATATTGGACCGAATGAAAGTAGCTCAACAGAAATT
ATTGAGAGCAACTTGAGAATGATTTCATTGTTGAAAAACAAAAATAGTGACATCATTAAT
AGTATCATCACACTATGGAAGCAAGCTCATCGTGATGTGTACAAATATTATGAGATGTGT
GCTAGTGCTTACTTTAAATTTCTTTTATTATCATCAAGTAATACCGACATGAATAGCGAT
TCGTCAGTTGTCACTGCTACACTTCGCTTATTGCGGTTGATTGTAAAACATGCGTTAGGT
CTGCAAGAAATTTTAGAAGAAGGTTTAGCGATAACTCCTTCAGGTCCCTGGAAAGTGATA
ATACCACAATTATTTTCACGATTGGATCATCATGAGCCTTACGTGAGAAAACGTGTAAGC
GAACTTTTATGTCGTGTAGCTAGCGACTCACCTCATTTAATCATTTTTCCAACTGTTGTC
GGAGCGGCTCATAATCATTTAGAAGAAGAAAAGTTTAAAGCTCAGCCAATTGAAGACAGC
AAAATCTTGAGCGATTGCAAAAAGTTTCTCAATTCGAGCTTATCTTACTGCTTCACTTCA
CTTCTCAACACATTATCGATGCAAAGCCCTCAAACTGTCAGCCAAGTGCAACTTCTTGTT
AACGAACTTCGCAGAATTTCCATGTTGTACGACGAAATGTGGCTCACAACTCTTAACCAA
CTCTATGTTGAAAATGCCAAAAAGTTTGTGAACTTTGATGCAGAATTTCAAGCTACTGAC
AAGTCGCAAGAGAAAATAAATTTATTCGCGGAAAAATACAAATTGCTAACGAAACAAATG
TTATTCATCATGGAATGCATGCAGGATTGGACATCCAAACCGCCAGAAACAAACAATGAG
CGCGCTTTTCAAGAGAAATTTTCGCATTTTATCGAAATGACAATTGGCGAGTTGAAAAGA
CCATTCGACTCAAGTGAACCGCTTGCTGCATTCAACAAATTCAAATCTTTATTGCATTTG
ATGCAACAGCGTGTACATAAGCGTATGAATCACACGTTAAAGATGAGTGATATTTCACCA
ATTCTCGCGAATTTAAATAACACAAATATTTCAATGCCAGGGGTTTTGCCTTCAAATGAT
AGCGAAGCAGTTTACATTCAGTCAGTCGACGAGACAATTCAGATTTTACCAACAAAAACA
AAACCCAAAAAGCTCGCATTTTATGGCAGTGATGGTAAACGTTATACATTTCTATTCAAG
GGCATGGAAGATTTGCATTTGGATGAACGCATTATGCAGTTCTTGTCGATTGCTAATTTA
ATGATGAAAAAATCAGTGGATTCTGATGGTAAAGTCACTTACTATCGAGCACATCATTAT
TCTGTCATTCCATTAGGACCAAGATCTGGTTTAATATCATGGGTTGATGGCGTGATTCCA
ATATTTTCCATTTACAAAAAATGGCAACAAAGAGAAGCCGCAAATCCTCGGAAAGACAAG
CAATACGTTGTCATGCGACCATCAGAGTTATTTTATAGCAAAATTACGCCTCTTCTGAAA
GAACATGGCCTGAAGCCAACTGACAATCGAAAGGACTGGCCTGTGTCTGTTCAAATGAAA
GCTTATGAAGAACTCAAGTCAGAAACTCCACGAGATTTACTAGCAAAAGAATTTTGGTGC
ACTGCATCGACTTCTGCACATTGGCGACAAATAGTTAGAAATTATTCAGTGTCACTTGCA
GTAATGAGCGTAATTGGTTATATTATTGGGTTGGGCGATAGGCATTTAGATAATATCCTT
GTCAAGTTAGCTAGTGGAGACATTGTCCACATTGACTACAATGTGTGTTTTGAAAAAGGC
AAAACATTGCGAGTCCCAGAACGAGTTCCATTCAGAATGACACAAAACATTGAAGAAGCT
TTAGGACTAACAGGCATTGAGCTTGCTGTTGATGATAGTGGTTTTGATGAAGTTTTGAAT
CGTATTATTTTTATTACTGTAACGATGATATGCCTTGAATATATTTATTTAGAACTTTTT
CTTCCCTATGGAATTTTTCTTTCTTCGAATGACTTCTCTAATACACGTACATATAACATC
ATAAGTGACTTTATGCCGAAAGTATTTTATCTTACATCTGAGATGACTTGCCATCTTGAC
AGTGATTGA
>g12129.t1 Gene=g12129 Length=2222
MDKYGESKSNRNRWKDEHRHPKLYDERLDTPGQQQNGKITTASSSSSSSKTQSSNSSSNK
LLKTDIVNQLAQNPNLPEDVRISKLLRRLETETTVTGTIEICGKLKTVVQDQANANYIKR
SFDMLTNSLISVMKECHTGEAVNHVAEVYGMMGFVMRNDFPLYKSWICKTYKNVKTLRVA
MMKALEKTLTMDSNELRLSDQIGRLMELLRDFLEQTDDYRVFMAITNVLIVIANNYNMRY
FKSHFTNVVDIVIGWMMENQLTKVKVHCAVVLQSLGQFWKNDSQFSLDLIGQLLEDIDGC
IEKMEDSNEDKVKNFKDFGAFVGVFNAIIKSVYVSPEALIYFMGKSTMEGCKEKIFRVAQ
FALNNENCIKETVLPVNEYILIVLNCRKCEIGIEMNSILTLIEIEMENLTKFTESELASF
LCVVNEFIKEFKSELPVQFIEKLFNVDSVLIQSVSFNNDKRVKSGLIKIYHEVLALKNVP
LLQAAYRCIIEQFGVCLKRIPQLKEIKWISDVVSNLVNNEITNEKTILRAQVALNFNLTA
LSKLATTQNSIIAMYSLVPSILEVLINLQIWRSEWNEFELTQYAILLVISEHCLKNNNFI
SSSALLINKTSTITTSSWLSDSSPTESPVSQHFKLILEFIDKMLKSSPSLKQFKLILDWL
DKIIQQTCQYSEQLKENSSFVSILKRINQIANNYKNDEVMLKVATCNDSLYSFESIHNEI
YTAMSEAGVVQMCSTNPEIRRRYSFILSRIPLKFTLEQAKSPSGINQETINKITEMENWH
LSLGSIHGGELRAQYFQKFINHITYSPNAANIDQFILHAFRNCWFNGTEMAEEYKRVTLK
DVRTLYSWMQWEAARFCVNNKLRTPLGKAQDTFIKIEGIIREHARILTLKDKSKLRGYEH
VLANQRNVRILLGFMESLEKAIYNAAEGSAFAIPPPEKPARTFFRLNASTCNEWFNRNRL
AIHQLALHALELEMVIRCSQSILKEMCAAGKINETYFDQILMSLTWAMLRNFESDSLCGL
YVWVKNVSGRKLHWIKMAAEQAAGHREVAADGYTKVLKDEKLEPKIYDFIADQRRCCLLF
TGNMEELYNVLVEEEERNYKPTNIPILKITKEQVASIKKYDKTKDFDALNDLSLWETLED
EGNVSNNFSVHNLLSLTENTLSCAILDKENSNRDKEKTSWEILHTLLQESIRTSSQEYLI
GLNVLNHIAHKYKIVINNEGSVNIESLEIEKKYGSLTMFFVSIYGEFLDACSEKGEQQNI
NLKLDLVASARKELNFRQCTRELSSVYRSVEYTEHIGLPFEHELSSIKEFFMSPRNVKDS
KIWSENVSRAIYEHCKMLYVAKNQHFDAIQFASTAALGIKNRLNITDGNVSENLNQQCVK
FYTKISEWIQNEPEESLFSHDGNSLNSLVQSINDEEYIDEQMPLVDSAVGKLLQSGVKQC
PEMPKAWSSLANWCYKWGRKMIESKTDSQGLRAIDSSAIVDLIPEAQPEDVESILKVLNE
QQIMVEDEDIGPNESSSTEIIESNLRMISLLKNKNSDIINSIITLWKQAHRDVYKYYEMC
ASAYFKFLLLSSSNTDMNSDSSVVTATLRLLRLIVKHALGLQEILEEGLAITPSGPWKVI
IPQLFSRLDHHEPYVRKRVSELLCRVASDSPHLIIFPTVVGAAHNHLEEEKFKAQPIEDS
KILSDCKKFLNSSLSYCFTSLLNTLSMQSPQTVSQVQLLVNELRRISMLYDEMWLTTLNQ
LYVENAKKFVNFDAEFQATDKSQEKINLFAEKYKLLTKQMLFIMECMQDWTSKPPETNNE
RAFQEKFSHFIEMTIGELKRPFDSSEPLAAFNKFKSLLHLMQQRVHKRMNHTLKMSDISP
ILANLNNTNISMPGVLPSNDSEAVYIQSVDETIQILPTKTKPKKLAFYGSDGKRYTFLFK
GMEDLHLDERIMQFLSIANLMMKKSVDSDGKVTYYRAHHYSVIPLGPRSGLISWVDGVIP
IFSIYKKWQQREAANPRKDKQYVVMRPSELFYSKITPLLKEHGLKPTDNRKDWPVSVQMK
AYEELKSETPRDLLAKEFWCTASTSAHWRQIVRNYSVSLAVMSVIGYIIGLGDRHLDNIL
VKLASGDIVHIDYNVCFEKGKTLRVPERVPFRMTQNIEEALGLTGIELAVDDSGFDEVLN
RIIFITVTMICLEYIYLELFLPYGIFLSSNDFSNTRTYNIISDFMPKVFYLTSEMTCHLD
SD
Transcript | Database | ID | Name | Start | End | E.value | |
---|---|---|---|---|---|---|---|
7 | g12129.t1 | Gene3D | G3DSA:3.30.1010.10 | - | 1828 | 1976 | 4.0E-12 |
8 | g12129.t1 | Gene3D | G3DSA:1.10.1070.11 | - | 2035 | 2165 | 2.5E-24 |
17 | g12129.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 27 | - |
18 | g12129.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 60 | - |
16 | g12129.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 29 | 60 | - |
3 | g12129.t1 | PANTHER | PTHR11139:SF108 | AGAP000368-PA | 104 | 2131 | 1.1E-275 |
4 | g12129.t1 | PANTHER | PTHR11139 | ATAXIA TELANGIECTASIA MUTATED ATM -RELATED | 104 | 2131 | 1.1E-275 |
2 | g12129.t1 | Pfam | PF15785 | Serine/threonine-protein kinase smg-1 | 554 | 1138 | 1.1E-124 |
1 | g12129.t1 | Pfam | PF00454 | Phosphatidylinositol 3- and 4-kinase | 1915 | 2147 | 3.6E-41 |
10 | g12129.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 2161 | - |
11 | g12129.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2162 | 2186 | - |
9 | g12129.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 2187 | 2222 | - |
13 | g12129.t1 | ProSitePatterns | PS00916 | Phosphatidylinositol 3- and 4-kinases signature 2. | 2078 | 2098 | - |
20 | g12129.t1 | ProSiteProfiles | PS51189 | FAT domain profile. | 1364 | 1664 | 10.089 |
19 | g12129.t1 | ProSiteProfiles | PS50290 | Phosphatidylinositol 3- and 4-kinases family profile. | 1915 | 2137 | 32.374 |
14 | g12129.t1 | SMART | SM01345 | Rapamycin_bind_3 | 1722 | 1844 | 3.4E-4 |
15 | g12129.t1 | SMART | SM00146 | pi3k_hr1_6 | 1916 | 2222 | 5.6E-13 |
5 | g12129.t1 | SUPERFAMILY | SSF48371 | ARM repeat | 1527 | 1684 | 3.96E-5 |
6 | g12129.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1830 | 2147 | 8.77E-67 |
12 | g12129.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2165 | 2187 | - |
IUPRED3 score over 0.5 is predictive of a disordered region.
GOID | TERM | ONTOLOGY |
---|---|---|
GO:0016310 | phosphorylation | BP |
GO:0004674 | protein serine/threonine kinase activity | MF |
GO:0016301 | kinase activity | MF |
GO:0000184 | nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | BP |
TPM values are indicated as average +/- STDEV.
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.