General information for g12251.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Activin receptor type-2A.
Parent gene
- The parent gene g12251 is located on chr_1: 22006495..22008263 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12251 | g12251.t1 | isoform | g12251.t1 | 22006495 | 22008263 |
| chr_1 | g12251 | g12251.t1 | exon | g12251.t1.exon1 | 22006495 | 22006665 |
| chr_1 | g12251 | g12251.t1 | cds | g12251.t1.CDS1 | 22006495 | 22006665 |
| chr_1 | g12251 | g12251.t1 | exon | g12251.t1.exon2 | 22006720 | 22006952 |
| chr_1 | g12251 | g12251.t1 | cds | g12251.t1.CDS2 | 22006720 | 22006952 |
| chr_1 | g12251 | g12251.t1 | exon | g12251.t1.exon3 | 22007017 | 22008144 |
| chr_1 | g12251 | g12251.t1 | cds | g12251.t1.CDS3 | 22007017 | 22008144 |
| chr_1 | g12251 | g12251.t1 | exon | g12251.t1.exon4 | 22008212 | 22008263 |
| chr_1 | g12251 | g12251.t1 | cds | g12251.t1.CDS4 | 22008212 | 22008263 |
| chr_1 | g12251 | g12251.t1 | TSS | g12251.t1 | 22008656 | 22008656 |
| chr_1 | g12251 | g12251.t1 | TTS | g12251.t1 | NA | NA |
Sequences
>g12251.t1 Gene=g12251 Length=1584
ATGCTCACAAGAATTTTACTTCTATTTCTTTTTCTAACAATATTTGTATCGGATGCAGCT
GCTTTTAATGACGATCTTGACCATAATGAAGATGAAAATTATGATTCATTTCATCCTGAT
TTAGTTATCACTTGCAAATATTTTAAACGTAAAGATTGTCAAGAGAAAGGTTTATGCAAA
GAAGAGAACAAAAAATGCCCAGCACCGAATGATAAACGACAAGGATGTTTTGTATTATGG
AAGCATGATAATATTACAAATGAAAATTATGTGGAAATGAAAGATTGCTTTATATTCCAT
GAAAATTGTGAACAAAATGAGTGCTACGATACAGATTATAATAAGACAAATGAAAACTAC
TTTTGTTGCTGCAGACATGATATGTGCAATGAAAAATTCGAATATAAGCCAGTTTTTAAA
GAAACAACAACTATTATAAATGAAGATTCTTCTGTAATAATTGATACTGAAACTGATACA
CCCAAATGGATTCTTGCATGTATTTTGATCCTTTCACTTATTGGATTTTGTTTCGCATTC
AAATATATTTATCATCGCAAGAAGGGACTTTTTAATGAAATTCCAACAATGGATCCAGAA
ATTTCAATTTCTTCACAATGCCTTGCAATTCGTCCAATTGATTTGATTGAAATTAAAGCT
CATGGTCGTTTTGGAACAGTTTGGAGAGGTAAAATGAAAACAGATGACGTTGCAGTAAAA
ATTTTCCCTACACAGGATAAAAATTCTTGGATTGCTGAACAGGAAATTTACAAACTTCCA
CGCATGAATCATTCAAATATTTTGCATTTTATTGGAGCTGAAAAACACATTTATCAGGAA
GGAAAAACTGAATTTTGGCTTATTACCGAATATCAACCAATTGGTTCATTGTGTGATTAT
TTAAAGTCTCACACCGTAACATGGTCAGAACTTTGCAAAATTGCTGAATCAATGGCTCGC
GGCTTGATGCACTTACATGAAGAAATTCCCGGCAATCGAAACGATTTAAAACCAGCAATT
GCTCATCGCGACTTTAAAAGTAAAAATGTTCTACTCAAATCTGATTTAACAGCTTGTATT
GCCGATTTTGGTCTCGCTCTTGTTTTTAATCCCGGTCAGCCTTGTGGTGATGTTCATGGA
CAAGTTGGAACTCGAAGATATATGGCTCCTGAAATTTTAGAAGGAGCAATAAATTTCAAT
CGCGATTCATTTTTGAGAATTGATGTTTATGCATGTGGTTTAGTGTTATGGGAATTAGTT
TCACGTTGCACTTGTCATGGTGGACCTGTTGCCGAATATCAATTGCCTTTTGAAAATGAG
CTTGGATCACATCCAACTTTGGAAGAAATGCAGGAAAATATTGCCGCCAAAAAATTGCGT
CCAAAACTTTACGATGAATGGAGAAATCACAATGGTTTAAGCGCAATTATTGATACGATT
GAAGAATGTTGGGATCATGACGCAGAAGCTCGATTATCATCGTCTTGCATAATGGAAAGA
ATTTCACAACACTCAAGATATCAAACGATAACAATTGATCAGACAACAAACTTTATAAGC
AGAAATAATGCGAATGATCAATGA
>g12251.t1 Gene=g12251 Length=527
MLTRILLLFLFLTIFVSDAAAFNDDLDHNEDENYDSFHPDLVITCKYFKRKDCQEKGLCK
EENKKCPAPNDKRQGCFVLWKHDNITNENYVEMKDCFIFHENCEQNECYDTDYNKTNENY
FCCCRHDMCNEKFEYKPVFKETTTIINEDSSVIIDTETDTPKWILACILILSLIGFCFAF
KYIYHRKKGLFNEIPTMDPEISISSQCLAIRPIDLIEIKAHGRFGTVWRGKMKTDDVAVK
IFPTQDKNSWIAEQEIYKLPRMNHSNILHFIGAEKHIYQEGKTEFWLITEYQPIGSLCDY
LKSHTVTWSELCKIAESMARGLMHLHEEIPGNRNDLKPAIAHRDFKSKNVLLKSDLTACI
ADFGLALVFNPGQPCGDVHGQVGTRRYMAPEILEGAINFNRDSFLRIDVYACGLVLWELV
SRCTCHGGPVAEYQLPFENELGSHPTLEEMQENIAAKKLRPKLYDEWRNHNGLSAIIDTI
EECWDHDAEARLSSSCIMERISQHSRYQTITIDQTTNFISRNNANDQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g12251.t1 | CDD | cd14053 | STKc_ACVR2 | 217 | 507 | 0.0 |
| 10 | g12251.t1 | Gene3D | G3DSA:2.10.60.10 | CD59 | 42 | 140 | 7.5E-13 |
| 11 | g12251.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 200 | 292 | 6.5E-33 |
| 12 | g12251.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 294 | 508 | 7.5E-57 |
| 2 | g12251.t1 | PANTHER | PTHR23255 | TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II | 41 | 509 | 6.0E-166 |
| 3 | g12251.t1 | PANTHER | PTHR23255:SF98 | SERINE/THREONINE-PROTEIN KINASE RECEPTOR | 41 | 509 | 6.0E-166 |
| 21 | g12251.t1 | PIRSF | PIRSF037393 | TGFRII | 1 | 211 | 0.035 |
| 20 | g12251.t1 | PIRSF | PIRSF037393 | TGFRII | 200 | 519 | 6.5E-80 |
| 5 | g12251.t1 | PRINTS | PR00653 | Activin type II receptor signature | 89 | 108 | 2.5E-5 |
| 4 | g12251.t1 | PRINTS | PR00653 | Activin type II receptor signature | 472 | 491 | 2.5E-5 |
| 1 | g12251.t1 | Pfam | PF00069 | Protein kinase domain | 222 | 498 | 9.5E-35 |
| 15 | g12251.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 16 | g12251.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 17 | g12251.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 19 | g12251.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 14 | g12251.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 162 | - |
| 18 | g12251.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 163 | 184 | - |
| 13 | g12251.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 185 | 527 | - |
| 25 | g12251.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 340 | 352 | - |
| 26 | g12251.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 213 | 507 | 38.04 |
| 6 | g12251.t1 | SUPERFAMILY | SSF57302 | Snake toxin-like | 44 | 137 | 1.28E-10 |
| 7 | g12251.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 195 | 500 | 1.5E-62 |
| 9 | g12251.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 23 | g12251.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 8 | g12251.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 21 | - |
| 24 | g12251.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 163 | 185 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0038023 | signaling receptor activity | MF |
| GO:0016020 | membrane | CC |
| GO:0005524 | ATP binding | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0007178 | transmembrane receptor protein serine/threonine kinase signaling pathway | BP |
| GO:0043235 | receptor complex | CC |
| GO:0046872 | metal ion binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0004675 | transmembrane receptor protein serine/threonine kinase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13596 (activin receptor type-2B)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
