Gene loci information

Transcript annotation

  • This transcript has been annotated as Elongation factor 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g12287 g12287.t28 isoform g12287.t28 22172624 22173484
chr_1 g12287 g12287.t28 exon g12287.t28.exon1 22172624 22173484
chr_1 g12287 g12287.t28 cds g12287.t28.CDS1 22172751 22173107
chr_1 g12287 g12287.t28 TTS g12287.t28 22173564 22173564
chr_1 g12287 g12287.t28 TSS g12287.t28 NA NA

Sequences

>g12287.t28 Gene=g12287 Length=861
AGCTGTTCGTTTCAACATTTATGATGTTACACTGCATGCTGACGCTATCCATCGTGGAGG
TGGTCAAATCATTCCAACAACTCGTCGTTGCTTGTATGCATCATACATCACAGCTTCTCC
ACGTTTGATGGAGCCAGTTTATTTGTGTGAAATTCAATGTCCAGAAGTTGCTGTCGGTGG
TATTTATGGTGTGCTTAATCGTCGTCGTGGTCATGTCTTTGAAGAATCACAAGTTGCCGG
TACACCTATGTTCGTTGTCAAAGCTTATTTGCCTGTAAATGAATCATTTGGATTCACTGC
TGATTTGCGCTCAAATACTGGAGGTCAAGCTTTCCCACAATGTGTATTTGACCATTGGCA
GATCTTCCCAGGTGACCCTAACGAACCATCATCAAAACCATACCAGATTGTGCAAGATAC
AAGAAAACGTAAAGGTCTCAAAGAAGGTTTACCAGATTTGAACCAATACTATGATAAAAT
GTAAAAGTCTTTCCGAGCTTCTTCAGTTATACTTATTATTTTTTCCCTCTTCATTTTTTG
CTTATAATTTTTTGATTTTTATATTTTTATCATAACATTACGCATAAAAGATAAAACAAC
ATCAAAGCAAGAAAAAAGCGTTGATTATTTTATATCAATTAATCGTAATATTGTAATCAA
ACTTAAAATATTTTTTGAGCGTCTTAATATGAGTTAAGTTAAAAGCATTCTCCTATGAAA
ATTAAAAGCGTTTATGAGTTCGCCATAAATATGCTGTTACATCTACGCGCGAAAGTGGTT
GAAATGAAAAAGAATTTCATATAAATATTAACAATGATATCCAGCAACATGGAAGAAAAA
TTAGAACGACGGAAACGGAAA

>g12287.t28 Gene=g12287 Length=118
MEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADL
RSNTGGQAFPQCVFDHWQIFPGDPNEPSSKPYQIVQDTRKRKGLKEGLPDLNQYYDKM

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g12287.t28 CDD cd04096 eEF2_snRNP_like_C 2 81 0
6 g12287.t28 Gene3D G3DSA:3.30.70.240 - 1 118 0
2 g12287.t28 PANTHER PTHR42908 TRANSLATION ELONGATION FACTOR-RELATED 1 118 0
3 g12287.t28 PANTHER PTHR42908:SF28 EUKARYOTIC TRANSLATION ELONGATION FACTOR 2,-LIKE 2-RELATED 1 118 0
1 g12287.t28 Pfam PF00679 Elongation factor G C-terminus 1 85 0
5 g12287.t28 SMART SM00838 EFG_C_a 1 88 0
4 g12287.t28 SUPERFAMILY SSF54980 EF-G C-terminal domain-like 2 116 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values