General information for g12527.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g12527 is located on chr_1: 24251772..24255140 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12527 | g12527.t1 | isoform | g12527.t1 | 24251772 | 24255140 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon1 | 24251772 | 24251808 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS1 | 24251772 | 24251808 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon2 | 24251903 | 24252461 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS2 | 24251903 | 24252461 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon3 | 24252527 | 24252899 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS3 | 24252527 | 24252899 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon4 | 24252958 | 24253071 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS4 | 24252958 | 24253071 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon5 | 24253137 | 24253149 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS5 | 24253137 | 24253149 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon6 | 24253217 | 24253264 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS6 | 24253217 | 24253264 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon7 | 24253326 | 24253517 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS7 | 24253326 | 24253517 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon8 | 24253572 | 24254236 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS8 | 24253572 | 24254236 |
| chr_1 | g12527 | g12527.t1 | exon | g12527.t1.exon9 | 24254301 | 24255140 |
| chr_1 | g12527 | g12527.t1 | cds | g12527.t1.CDS9 | 24254301 | 24255140 |
| chr_1 | g12527 | g12527.t1 | TTS | g12527.t1 | 24255348 | 24255348 |
| chr_1 | g12527 | g12527.t1 | TSS | g12527.t1 | NA | NA |
Sequences
>g12527.t1 Gene=g12527 Length=2841
ATGATAAGAATTATCAGATTACCTGATTGCTATTTAATGATAATCGGGAATGTAGAGTTG
AAAAAAACACCACCTTATATAGCAAGAAAATTAGCTTTGCAAGAATCCGGCTTTAGTCTT
GCTAGACCAACGTCAGACGTTGAGGCTCAAGCAGATGTCATCAATTATCGCTTACCAAAC
AATACTAAACCATTGTATTATGACATTCATATGACAACTCATATTCATGAAAATAAATTT
GAGTTTAATGGTCATGTGATTATAAAGTTTGAAGTTAAAACTTTACCATTAAATGAAATT
GTAATTCATATGAAAGAATTAGAAAATCTTGAAATAAAACTTTTAAATTCAACTCTTGGA
TTGATTGAAAATAATGTGGATTATGATCATGAAGAAGAAACTGATTTTCTCATAATTAAA
ACAAAAAATAATCTGGTTTTAAATGGAATTTATCATCTTGATATAACATATGATGGTGAA
TTAAGAGACGACAATTTGGGTTTTTATAGATCTTCATATTTAAATGACAAGAATGAAAAT
ATTTGGCTAGCAACGACACAATTTGAAAGTGTAGAAGCAAGAAGTGCTTTCCCATGCTAT
GATGAACCGCAAATTCGCGCACCTTTTAAAATTAAGATAACATATGGAAAAAATTACAAA
GCTGTTTCAAACATGCCAGTAATAGAAACAATCGATGAGAGTAGCTTACAGGGCTACGTG
ACCACATCATTTAAAGAAAGTCCTCCCATACAAACATATCTGATTGCTTTCATCATTTCT
GACTTTGATTATGTGTCGAAAGGTGTACAAAAAGTTTATGCAAAACCCAATTCTATTAAA
GATGGTGAAGCTGATCTTGCATTACGTTATGGAATAGAAATTATAGCTAAATTCGAAGAG
TATTTGGGTGTTGATTATTTTACAGAGAAAATGGATCAATTTGCCCTACCCGATTTTGAT
GCTGGTGCTATGGAGAATTTTGGAATTGTAACTTATAGAGAAGAATATTTACTACATAAT
AATGCAACTGACACAACTCAACAAAAAGAAAATGTTTTGACAATAATTTCACACGAATTT
GCTCACCAATGGTTTGGTGATTTAGTTGCTCCAGACTGGTGGGTTTATTTGTGGCTGAAT
GAAGGTTTTGCGACTCTTTATGAATTTCAACTAGTCAGCTTAATTTATGAAACTGATCGT
TTAATGGATTCATTTATATTAAATGCTGTTCATCCTGCATTAGAATATGATGGCAATGAT
GTTAGACCTATGTCACATTATGTTGAAAGTCCTGATGATATTGATGATTTATTTGATTAT
ATTGCTTATGAAAAAGCTGGAAGTGTTTTGCGAATGATTCAAAATGCAATTGGTGATGAT
GTATGGAGCAAAGGTTTAGAAAATTATCTTAAAAATCGTTCATTACTTGCGGCAACACCA
GATCATCTTCATACAGCTATTCAACAAGCAATAAATGAAAAAGGAAACATTGATCTTGAC
ATTGCAAAAATTATGCTCTCATGGGAAACTCAATCAGGTTATCCAGTGATAAATGTGAAA
AAAACAAATGACACACTTACCTTTACTCAAGAACGTTTTTTATATGATGAAGAAGATAAA
GAAACTCTTTGGTATGTTCCGATAAATTTCATCACTGCATCTGACTCATTAGTCATAAAT
ACAAAACCAGATTTGTGGTTAAATCAAGAGAGATCAATCTCACTTTCAAGAGTAAAATCA
TCAAGAAATTGGAATGAAAAAGATTGGATTGTCGCAAACTTGCAGCAAGGTTATTATTAT
CGTGTAAATTACGATGAAAGTATCTGGCAAGCACTTATCGATCAATTAAATGAATGTCAT
AATGTTATCGATTTGCGCAGTCGTGGTCAATTAATTGATGATTCACTGAATCTGGCGCGT
GCCGGTAAAATTTCATATGAAATTCCTTTCGGTATTGTAAAACACCTGAAGAAGGAAACA
GATTATTTGCCATGGGCTGCGGCCTACGGTGACTTGTTACTACTGAATCGTTGGCTAAGT
GGCTTGAACATGCACGCAAAGTATCAAAAATATATTCGCACTCTTGTTGAGCCATTTTAT
ACACATGTCGGTTTTGAGATTATGGAGAATGAGCACAAATTCAATCGCTACTCTCGAAGA
ATTGCTATAAATCTCGCGTGTCAATTTGGTCATGATGATTGTCTCGAAACAACAAAGTCA
AAATTGAAAGATGAAAAGATTCATCCGGATATGCAGGAAGCAATTTATTCAAATGGATTG
AGAGGAGCTGATGCGGAAACTTACAACAAAACAATCGAAATGATGTTGAATTTAGAGAAT
CAGGCTCAAAGAACATTAATCATAAGAGCATTAGGATGCGCTCAAAATGAAGCAAATTTA
AAAACTTTCTTGTTATTAGCTATTAAAGATGAACCAAAAATTCGACTTCAAGAAAAATAT
AGAATCTTCAGTTCATGGACAAATGCTGGTCACGTAGGTGTAAAAGTTGTAATAGATTTT
GTTAAAGAGCATTATGAAGACATCAGTGACATTTCATCATCTTTAGTCAGATCAATTCTG
AACAATGCCGCTTCTTATATTTCATCTGAAGAATTGACTACTCAATTCATTGATCTCCTT
GACTTCTTAAAAGCCAAAAATGTTATTGATGATACCCATAAAAATGACTATTTAAATACG
GTGAACGCCACAATTTTGTGGCAAGAAAAAAATGTTAAAGTTATTGAAGACTGGCTTGAT
CGTAATCGCGCAAACTCTGTTGTTATATCGACAGCAGTACTTGCTTTGGTTTTTTCCGCA
ATAATAAAATTGTTTTTCTAG
>g12527.t1 Gene=g12527 Length=946
MIRIIRLPDCYLMIIGNVELKKTPPYIARKLALQESGFSLARPTSDVEAQADVINYRLPN
NTKPLYYDIHMTTHIHENKFEFNGHVIIKFEVKTLPLNEIVIHMKELENLEIKLLNSTLG
LIENNVDYDHEEETDFLIIKTKNNLVLNGIYHLDITYDGELRDDNLGFYRSSYLNDKNEN
IWLATTQFESVEARSAFPCYDEPQIRAPFKIKITYGKNYKAVSNMPVIETIDESSLQGYV
TTSFKESPPIQTYLIAFIISDFDYVSKGVQKVYAKPNSIKDGEADLALRYGIEIIAKFEE
YLGVDYFTEKMDQFALPDFDAGAMENFGIVTYREEYLLHNNATDTTQQKENVLTIISHEF
AHQWFGDLVAPDWWVYLWLNEGFATLYEFQLVSLIYETDRLMDSFILNAVHPALEYDGND
VRPMSHYVESPDDIDDLFDYIAYEKAGSVLRMIQNAIGDDVWSKGLENYLKNRSLLAATP
DHLHTAIQQAINEKGNIDLDIAKIMLSWETQSGYPVINVKKTNDTLTFTQERFLYDEEDK
ETLWYVPINFITASDSLVINTKPDLWLNQERSISLSRVKSSRNWNEKDWIVANLQQGYYY
RVNYDESIWQALIDQLNECHNVIDLRSRGQLIDDSLNLARAGKISYEIPFGIVKHLKKET
DYLPWAAAYGDLLLLNRWLSGLNMHAKYQKYIRTLVEPFYTHVGFEIMENEHKFNRYSRR
IAINLACQFGHDDCLETTKSKLKDEKIHPDMQEAIYSNGLRGADAETYNKTIEMMLNLEN
QAQRTLIIRALGCAQNEANLKTFLLLAIKDEPKIRLQEKYRIFSSWTNAGHVGVKVVIDF
VKEHYEDISDISSSLVRSILNNAASYISSEELTTQFIDLLDFLKAKNVIDDTHKNDYLNT
VNATILWQEKNVKVIEDWLDRNRANSVVISTAVLALVFSAIIKLFF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 20 | g12527.t1 | CDD | cd09601 | M1_APN-Q_like | 65 | 508 | 5.236E-175 |
| 14 | g12527.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 46 | 261 | 2.3E-61 |
| 15 | g12527.t1 | Gene3D | G3DSA:1.10.390.60 | - | 264 | 513 | 1.5E-80 |
| 13 | g12527.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 514 | 604 | 7.1E-19 |
| 16 | g12527.t1 | Gene3D | G3DSA:1.25.50.20 | - | 605 | 921 | 7.1E-78 |
| 4 | g12527.t1 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 44 | 916 | 7.6E-250 |
| 5 | g12527.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 44 | 916 | 7.6E-250 |
| 6 | g12527.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 194 | 209 | 4.0E-25 |
| 9 | g12527.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 244 | 259 | 4.0E-25 |
| 8 | g12527.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 319 | 329 | 4.0E-25 |
| 10 | g12527.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 355 | 370 | 4.0E-25 |
| 7 | g12527.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 374 | 386 | 4.0E-25 |
| 3 | g12527.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 63 | 254 | 1.4E-43 |
| 2 | g12527.t1 | Pfam | PF01433 | Peptidase family M1 domain | 288 | 508 | 2.3E-63 |
| 1 | g12527.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 589 | 874 | 5.9E-53 |
| 18 | g12527.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 926 | - |
| 19 | g12527.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 927 | 945 | - |
| 17 | g12527.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 946 | 946 | - |
| 12 | g12527.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 50 | 261 | 1.28E-51 |
| 11 | g12527.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 271 | 519 | 1.47E-66 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11140 (aminopeptidase N)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed