Gene loci information

Transcript annotation

  • This transcript has been annotated as Phosphatidylinositide phosphatase SAC1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g12763 g12763.t1 TTS g12763.t1 25838028 25838028
chr_1 g12763 g12763.t1 isoform g12763.t1 25838250 25840333
chr_1 g12763 g12763.t1 exon g12763.t1.exon1 25838250 25838459
chr_1 g12763 g12763.t1 cds g12763.t1.CDS1 25838250 25838459
chr_1 g12763 g12763.t1 exon g12763.t1.exon2 25838519 25839966
chr_1 g12763 g12763.t1 cds g12763.t1.CDS2 25838519 25839966
chr_1 g12763 g12763.t1 exon g12763.t1.exon3 25840096 25840187
chr_1 g12763 g12763.t1 cds g12763.t1.CDS3 25840096 25840187
chr_1 g12763 g12763.t1 exon g12763.t1.exon4 25840308 25840333
chr_1 g12763 g12763.t1 cds g12763.t1.CDS4 25840308 25840333
chr_1 g12763 g12763.t1 TSS g12763.t1 25840439 25840439

Sequences

>g12763.t1 Gene=g12763 Length=1776
ATGGAGGTCCATGATGATATGAACTTCTACATCACAATAGACAAGTTCTTTATAGAACCT
AATGGGAAGAGCGAAGTATTAATTATAGACCGTCTAACTAAAGAGTTTAGTGTTCAAGTT
AGAACAAATCAAATTCCCAATACGTCAAGTGTGAGAAAAATATGTGGAATACTCGGAACA
ATTAACTTAATTTCGGGCACTCATTTAGTAGTAGCAACGCATCGAATAGCAGTTGGTTTT
ATAAATAATCAAGTAATATGGCGTCTCGCAGGATTCGATATTATTCAATACATTCCATCG
ATGCTTCATTTAAACGAAGTACAAAAGAAGGAGAATGAAAATTATTTGGCGATGATTCGT
ACAATTTTGGATACGCCATATCTTTATTTTAGTTACTCGTATGATTTAACACACTCGCTT
CAGCGGTTACATTCAATGTCGCCCGATCAACTGTCATTGAGTTTGTTTGAAAGAGCAGAT
CCACGATTCGTTTGGAATAGAAACTTGTTGAAACCTTTTCAAAGACCAGAGATGCGTCGT
TACTGTCTTCCTCTACTATTAGGATTTATATCAATTAATCAAGTGAGCATCAATGGTCAT
GTCTTTTCATGGATTTTAATTTCACGACGTTCAACTAGACGTGCTGGTACTCGTCTTTTC
TGTCGTGGGATTGATAATGATGGAAATGTCTCAAATTTTGTTGAGACTGAGCAAATTGTT
GAATACAATAGTGAAAAAGTGTCCTTTGTACAAATTCGCGGAAGTATACCGATGTTTTGG
TATCAAACCCCAAATCTTCGTCTTAAGCCACGTCCAAAAATCGATAAAACTCGTGATAGC
TTACCGCCATATCAAATTCACATGGATGAATTAGTGATGCACTATGGAAAAATTGTTCTT
GTCAATTTAATTGATCATCGTGGTGCTGAAGATGAGCTAGAAAAAGCATTTGCTCAACTT
GTAAGCAGAGCCAATAGTGTAAATGAACGCTATGAAGCTTTTGATTTTCATGCGGAATGT
AGAAAAATGCGTTGGGATAGATTACATATTTTAATTGATCGATTAGCACATGAACAAGAT
GAATTTGGAGTTTTTCATCTTCGGTCAGGAACATTGCATTCAGCTCAAGATGGTGTTTTT
AGAACAAACTGTATTGATTGTTTAGATCGAACAAATGTTGTGCAAAGTATGTTAGCGAAA
CGATCACTACAACAGACTTTAACGAAGCTTGGAATTCTATATAGTGGACAGACTCTAGAA
AGTGTCGGTAGTGGTATGATAGATTTGTTCAAAAATATTTGGGCTGACAATGCCGATTTA
ATTTCAATTCAATATTCTGGCACTGGTGCTTTAAAAACTGATTTCACTAGAACTGGCAAA
AGAACGAAGCAAGGATTGCTGAAAGATGGGATAAATTCATTGACACGATATTATAAAAAT
AATTTTAATGATGGATTTCGACAAGATGCTATTGACTTGTTTGTAGGAAACTATTTTGTT
AGAGATGGAGAAGGTGCAGCATTCCCTTGTCCGCTGCAAACACAAAAAGATTGGAAATAT
GGAACGTTCCCAAGTATTTTATTACTTGCAATTTCAATGTTCTTCGCAACTGTATTATTC
TCACATGAGTACAATACTGAATCATTATTGTTCCTAATGTTTTGGGGCGCAATGGTATTT
GGAACCGGAAGTATGATTTTACGTAATGGAAGAGAATTTGTTGATGCCCCAAAATTAAAT
ATTCTCACACTTCCACCGAAAGAATTTGGACCTTAG

>g12763.t1 Gene=g12763 Length=591
MEVHDDMNFYITIDKFFIEPNGKSEVLIIDRLTKEFSVQVRTNQIPNTSSVRKICGILGT
INLISGTHLVVATHRIAVGFINNQVIWRLAGFDIIQYIPSMLHLNEVQKKENENYLAMIR
TILDTPYLYFSYSYDLTHSLQRLHSMSPDQLSLSLFERADPRFVWNRNLLKPFQRPEMRR
YCLPLLLGFISINQVSINGHVFSWILISRRSTRRAGTRLFCRGIDNDGNVSNFVETEQIV
EYNSEKVSFVQIRGSIPMFWYQTPNLRLKPRPKIDKTRDSLPPYQIHMDELVMHYGKIVL
VNLIDHRGAEDELEKAFAQLVSRANSVNERYEAFDFHAECRKMRWDRLHILIDRLAHEQD
EFGVFHLRSGTLHSAQDGVFRTNCIDCLDRTNVVQSMLAKRSLQQTLTKLGILYSGQTLE
SVGSGMIDLFKNIWADNADLISIQYSGTGALKTDFTRTGKRTKQGLLKDGINSLTRYYKN
NFNDGFRQDAIDLFVGNYFVRDGEGAAFPCPLQTQKDWKYGTFPSILLLAISMFFATVLF
SHEYNTESLLFLMFWGAMVFGTGSMILRNGREFVDAPKLNILTLPPKEFGP

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g12763.t1 PANTHER PTHR45662 PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1 14 579 2.1E-182
1 g12763.t1 Pfam PF02383 SacI homology domain 56 341 5.0E-84
8 g12763.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 184 -
11 g12763.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 185 207 -
7 g12763.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 208 522 -
10 g12763.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 523 542 -
9 g12763.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 543 547 -
12 g12763.t1 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 548 567 -
6 g12763.t1 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 568 591 -
5 g12763.t1 ProSiteProfiles PS50275 Sac phosphatase domain profile. 119 447 74.892
3 g12763.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 520 542 -
4 g12763.t1 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 549 567 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0042578 phosphoric ester hydrolase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values