General information for g12821.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit STT3A.
Parent gene
- The parent gene g12821 is located on chr_1: 26432183..26433574 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12821 | g12821.t1 | TTS | g12821.t1 | 26428786 | 26428786 |
| chr_1 | g12821 | g12821.t1 | isoform | g12821.t1 | 26429064 | 26432183 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon1 | 26429064 | 26429598 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS1 | 26429064 | 26429598 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon2 | 26429745 | 26429761 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS2 | 26429745 | 26429761 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon3 | 26430071 | 26430193 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS3 | 26430071 | 26430193 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon4 | 26430248 | 26430650 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS4 | 26430248 | 26430650 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon5 | 26430709 | 26430811 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS5 | 26430709 | 26430811 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon6 | 26430866 | 26431378 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS6 | 26430866 | 26431378 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon7 | 26431440 | 26431681 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS7 | 26431440 | 26431681 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon8 | 26431736 | 26431815 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS8 | 26431736 | 26431815 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon9 | 26431882 | 26431925 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS9 | 26431882 | 26431925 |
| chr_1 | g12821 | g12821.t1 | exon | g12821.t1.exon10 | 26432084 | 26432183 |
| chr_1 | g12821 | g12821.t1 | cds | g12821.t1.CDS10 | 26432084 | 26432183 |
| chr_1 | g12821 | g12821.t1 | TSS | g12821.t1 | 26432456 | 26432456 |
Sequences
>g12821.t1 Gene=g12821 Length=2160
ATGAGTTCAAGCAGTAGTGCAAAGCAATCCTTTAAAATTAATTGGGAGAAACAAGAGAGC
TTAGTGAAATTAAGCATCTTAATTCTCGCTGCAATATTATCTTTTGCAACACGTCTCTTC
TCTGTTCTCCGTTTTGAATCAGTGATTCATGAATTCGATCCATACTTTAATTATCGAACT
ACCAAATTTCTCACTGAGAAAGGATTTTATAACTTCCACAACTGGTTCGATGAAATGGCT
TGGTATCCGCTTGGAAGAATTATCGGAGGAACAATTTATCCCGGCTTGATGGTGACTTCA
TCAGCAATTTATCGTATTTTATGGTTTTTCAATATGACTGTTGACATAAGAAACATTTGT
GTGTTTTTGGCTCCTCTTTTCTCCTCATTCACGACTATCGTCACATATTTACTAACTTCC
GAAATTTACTCGACGGGCGCTGGACTTGTTGCAGCTTCTTTCATTGCAATTGTTCCTGGT
TACATTTCAAGATCAGTAGCTGGTTCATATGATAACGAAGGAATTGCAATTTTTGCTATG
CTTCTTACTTATTATTTATGGATTAAAGCTGTTAAAACAGGCACAATTTGTTGGTCTGCT
CTTGCTAGTTTGGCATACTTTTATATGGTTTCTAGTTGGGGTGGTTACGTGTTTTTAATC
AACTTGATTCCTTTGCACGTGCTAGCTTTAATGATCACAGGAAGATTTTCACATCGTGTA
TATGTGGCATATAGCTCTGTTTATACAATCGGAACAATTTTGAGCATGCAAATCTCATTC
GTCGGATTTCAACCAGTTCAATCATCAGAACATATGCTTGCATTTGGAACATTTGGATTG
TGCCAACTTCATGCACTTGTTGATTTCCTCAAGTCAAAATTATCAAAAGAACAATTTGAA
GTATTATTCAAATCAATTGCTATAGCTATTCTTTCAATTTTCCTTGGTTTTGCTGCAGTT
CTTACTTTATCAGGAAAAATTTCACCTTGGACTGGACGTTTCTACTCTCTATTGGACCCT
TCATATGCCAAAAATCATATTCCTATCATTGCTTCTGTATCTGAGCATCAGCCTACAAGT
TGGTCATCATTCTATTTTGATCTTCAAATATTAGTTTTCTTATTCCCTGCTGGACTTTAC
TTCTGTTTTGATAAACTCACTGATGCCAACATCTTTATTATTCTCTATGGAGTTACCTCA
ATTTACTTTACAGGAGTTATGGTCCGATTAATGCTAGTTCTTGCACCAGTTATGTGCATT
CTCTCTGGAATTGCTGTTTCTCATTTGCTTTCAAAATACATAAGAAATGTGGATGCCTCA
GTTAGCGTATTGAGTAGCGATGGAAAGTCACAGCCACGTCAAGAATCTTCCAAAAAGTAC
AAGAAATATGATCAGCAACAAAAAGGAATTAAAAGTGAAATTGCTATTGGATTTGTTGTA
TTGACAACATTTATGTTGATAAGCTTTGTTTTCCATTCAGTTTGGGTTACTTCTGAGGCG
TATAGTTCACCATCAATTGTGTTATCAGCAAGATCTCACGATGGCAGTAGAATAATTTTT
GATGATTTCCGTGAAGCATATTATTGGCTAAGAATGAATACAGCTGAGGATGCAAATATT
GCAAGGTGGGATTATGGTTACCAAATAACTGCGATGGCAAATAGAACAGTGCTTGTGGAT
AATAATACTTGGAACAATACACATATTTCTCGAGTTGGACAAGCTATGGCATCAAGTGAA
GAAAAAGCGTATGAAATTTTACGTGAACTGGATGTCAATTATGTTTTGGTAATTTTTGGT
GGCTTGACTGGTTATTCATCTGATGATATCAATAAATTCTTGTGGATGGTGAGAATTGGT
GGTTCAACTGATCGTGGAAGTCATATTAAAGAACAAGATTATTATTCGTCGAATGGTGAC
TTTAGAGTTGATAAAGAAGGTTCTCAAACTTTATTGAATTGTTTAATGTACAAACTCTGT
TATTATCGCTTTGGTAATGTCTATACTGAAGCAGGAAAACCAACAGGTTATGATAGAGTT
AGAGGAGCGGAAATTGGTAACAAAGATTTTGAACTAGAAACACTTGAAGAAGTATACACA
ACTGAGCATTGGCTTGTGAGAATCTATAAAGTGAAAGATTTAGAAAATCGCGGCGTATAA
>g12821.t1 Gene=g12821 Length=719
MSSSSSAKQSFKINWEKQESLVKLSILILAAILSFATRLFSVLRFESVIHEFDPYFNYRT
TKFLTEKGFYNFHNWFDEMAWYPLGRIIGGTIYPGLMVTSSAIYRILWFFNMTVDIRNIC
VFLAPLFSSFTTIVTYLLTSEIYSTGAGLVAASFIAIVPGYISRSVAGSYDNEGIAIFAM
LLTYYLWIKAVKTGTICWSALASLAYFYMVSSWGGYVFLINLIPLHVLALMITGRFSHRV
YVAYSSVYTIGTILSMQISFVGFQPVQSSEHMLAFGTFGLCQLHALVDFLKSKLSKEQFE
VLFKSIAIAILSIFLGFAAVLTLSGKISPWTGRFYSLLDPSYAKNHIPIIASVSEHQPTS
WSSFYFDLQILVFLFPAGLYFCFDKLTDANIFIILYGVTSIYFTGVMVRLMLVLAPVMCI
LSGIAVSHLLSKYIRNVDASVSVLSSDGKSQPRQESSKKYKKYDQQQKGIKSEIAIGFVV
LTTFMLISFVFHSVWVTSEAYSSPSIVLSARSHDGSRIIFDDFREAYYWLRMNTAEDANI
ARWDYGYQITAMANRTVLVDNNTWNNTHISRVGQAMASSEEKAYEILRELDVNYVLVIFG
GLTGYSSDDINKFLWMVRIGGSTDRGSHIKEQDYYSSNGDFRVDKEGSQTLLNCLMYKLC
YYRFGNVYTEAGKPTGYDRVRGAEIGNKDFELETLEEVYTTEHWLVRIYKVKDLENRGV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g12821.t1 | Gene3D | G3DSA:3.40.50.12610 | - | 519 | 663 | 3.0E-31 |
| 2 | g12821.t1 | PANTHER | PTHR13872:SF43 | DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3A | 10 | 718 | 0.0 |
| 3 | g12821.t1 | PANTHER | PTHR13872 | DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT | 10 | 718 | 0.0 |
| 1 | g12821.t1 | Pfam | PF02516 | Oligosaccharyl transferase STT3 subunit | 24 | 502 | 1.1E-139 |
| 19 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 20 | - |
| 35 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 40 | - |
| 29 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 41 | 86 | - |
| 41 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 107 | - |
| 18 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 108 | 118 | - |
| 44 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 119 | 138 | - |
| 25 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 139 | 143 | - |
| 38 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 144 | 162 | - |
| 22 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 163 | 173 | - |
| 42 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 174 | 201 | - |
| 28 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 202 | 212 | - |
| 32 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 213 | 233 | - |
| 24 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 234 | 239 | - |
| 36 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 260 | - |
| 26 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 261 | 271 | - |
| 34 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 272 | 290 | - |
| 23 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 291 | 301 | - |
| 43 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 302 | 323 | - |
| 30 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 324 | 363 | - |
| 37 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 364 | 382 | - |
| 20 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 383 | 388 | - |
| 40 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 389 | 405 | - |
| 27 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 406 | 410 | - |
| 33 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 411 | 430 | - |
| 21 | g12821.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 431 | 473 | - |
| 39 | g12821.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 474 | 495 | - |
| 31 | g12821.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 496 | 719 | - |
| 11 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 42 | - |
| 4 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 109 | - |
| 14 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 116 | 138 | - |
| 7 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 142 | 161 | - |
| 16 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 174 | 191 | - |
| 5 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 206 | 228 | - |
| 12 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 262 | - |
| 6 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 272 | 289 | - |
| 13 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 301 | 323 | - |
| 10 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 361 | 383 | - |
| 9 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 390 | 407 | - |
| 8 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 411 | 430 | - |
| 15 | g12821.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 474 | 496 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006486 | protein glycosylation | BP |
| GO:0004576 | oligosaccharyl transferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07151 (dolichyl-diphosphooligosaccharide—protein glycosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
