General information for g12834.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Multifunctional protein ADE2.
Parent gene
- The parent gene g12834 is located on chr_1: 26473015..26477102 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12834 | g12834.t1 | TSS | g12834.t1 | 26471425 | 26471425 |
| chr_1 | g12834 | g12834.t1 | isoform | g12834.t1 | 26471536 | 26473015 |
| chr_1 | g12834 | g12834.t1 | exon | g12834.t1.exon1 | 26471536 | 26471554 |
| chr_1 | g12834 | g12834.t1 | cds | g12834.t1.CDS1 | 26471536 | 26471554 |
| chr_1 | g12834 | g12834.t1 | exon | g12834.t1.exon2 | 26471751 | 26473015 |
| chr_1 | g12834 | g12834.t1 | cds | g12834.t1.CDS2 | 26471751 | 26473015 |
| chr_1 | g12834 | g12834.t1 | TTS | g12834.t1 | 26473111 | 26473111 |
Sequences
>g12834.t1 Gene=g12834 Length=1284
ATGGCAGATATTAAACAAATCGGTGATTACAAACTCGGAAAACTTTTGAATGAAGGAAAG
ACTAAACAAATTTATGATCTTCCTAATAATCCGGGCTATGTCTTGCTATTAAATAAAGAT
AGAATCAGTGCTCATAATGGTGTCCGCGTTCACGATCTTGAGGGAAAAGCTGAAATTTCT
AATAAGACAAATGCAAAAGTTTTTGAGCTTTTGAATATTGCTGGAGTCAAAACAGCTTTT
GTTAAATTGGCTTCTGATAATGCATTTATTGCTCGCAAATGTGAGATGGTGCCGATTGAA
TGGGTCACAAGACGTTTGGCTACTGGTTCATTTTTGAAGAGAAATCCAGGAATTCAAGAA
GGACTTCGCTTCTGTCCACCAAAACAGGAAACATTTTTCAAAGATGATGCAAACGATGAT
CCACAATGGAGTGAAGAACAGATTGTAGCAGCAAAGTTCAAAGTCAATAACTTATTAATC
ACACAAGATGAAGTTGATATTATGAGACAAACAACAATTCTCGTCTTTGAAATCCTCGAA
CGTGCATGGACAGAGAAAAACTGTGCACTCATTGATATGAAAATTGAATTTGGAGTAGAT
GAAAACGGCGAAATACTTGTTGCTGATGTGATAGACAGCGATTCATGGCGTTTGTGGCCT
TCGGGTGATAAACGTTTGATGGTCGATAAGCAAGTATACAGAAATTTAACTACAGTTACA
AGTAAAGATTTGGAAACTGTCAAAAGAAATTTCCAATGGGTTCATGATCAATTGTCTGAA
ATTTTACCTAAAAATGATCATTTGGTTGTGATTATTATGGGTTCACCTTCTGATAAGGAA
CACTGTCAAAAAATTGCCAAACATTGTAATGAACTTGGACTTAATACAGAAATGCGCGTT
TCTTCAGCACACAAAGTTACAAAGGGCACACTTCAAATCATGTCAGAATATGAAGGAATT
ATCAAAAATCTCGTCTTTATCACAGTTGCTGGTCGTTCAAACGGACTCGGACCAGTTTTA
TCTGGCAACACATCTTATCCAGTGATTAACTGTCCACCAATCAAAAGTGATACCATGAAC
ATTGACTTGTGGTCTTCAATCAACTTGCCATCCGGTTTAGGATGTAGCACAGTAATCTAT
CCAGAAGCCGCAGCACTCAATGCAGCTCAAATTTTGGGTCTCAGCAACTTCATGATTTGG
TCACGACTTAGAGTCAAGATGATTAATAATCTGATAAGCTTGAAAAAAGCTGATAAAGAA
TTGCGTGGAGTCAAGGAAGCTTAA
>g12834.t1 Gene=g12834 Length=427
MADIKQIGDYKLGKLLNEGKTKQIYDLPNNPGYVLLLNKDRISAHNGVRVHDLEGKAEIS
NKTNAKVFELLNIAGVKTAFVKLASDNAFIARKCEMVPIEWVTRRLATGSFLKRNPGIQE
GLRFCPPKQETFFKDDANDDPQWSEEQIVAAKFKVNNLLITQDEVDIMRQTTILVFEILE
RAWTEKNCALIDMKIEFGVDENGEILVADVIDSDSWRLWPSGDKRLMVDKQVYRNLTTVT
SKDLETVKRNFQWVHDQLSEILPKNDHLVVIIMGSPSDKEHCQKIAKHCNELGLNTEMRV
SSAHKVTKGTLQIMSEYEGIIKNLVFITVAGRSNGLGPVLSGNTSYPVINCPPIKSDTMN
IDLWSSINLPSGLGCSTVIYPEAAALNAAQILGLSNFMIWSRLRVKMINNLISLKKADKE
LRGVKEA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g12834.t1 | CDD | cd01416 | SAICAR_synt_Ade5 | 10 | 258 | 3.76588E-160 |
| 10 | g12834.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 16 | 94 | 2.6E-81 |
| 9 | g12834.t1 | Gene3D | G3DSA:3.30.470.20 | - | 95 | 237 | 2.6E-81 |
| 11 | g12834.t1 | Gene3D | G3DSA:3.40.50.7700 | - | 267 | 395 | 1.3E-47 |
| 5 | g12834.t1 | Hamap | MF_00137 | Phosphoribosylaminoimidazole-succinocarboxamide synthase [purC]. | 13 | 326 | 20.844673 |
| 6 | g12834.t1 | Hamap | MF_02045 | Phosphoribosylaminoimidazole carboxylase [purE]. | 267 | 422 | 34.577671 |
| 3 | g12834.t1 | PANTHER | PTHR43599:SF3 | MULTIFUNCTIONAL PROTEIN ADE2 | 9 | 424 | 1.4E-193 |
| 4 | g12834.t1 | PANTHER | PTHR43599 | MULTIFUNCTIONAL PROTEIN ADE2 | 9 | 424 | 1.4E-193 |
| 1 | g12834.t1 | Pfam | PF01259 | SAICAR synthetase | 16 | 242 | 1.3E-54 |
| 2 | g12834.t1 | Pfam | PF00731 | AIR carboxylase | 269 | 405 | 6.8E-35 |
| 14 | g12834.t1 | ProSitePatterns | PS01057 | SAICAR synthetase signature 1. | 96 | 110 | - |
| 13 | g12834.t1 | ProSitePatterns | PS01058 | SAICAR synthetase signature 2. | 190 | 198 | - |
| 15 | g12834.t1 | SMART | SM01001 | AIRC_2 | 267 | 414 | 7.9E-28 |
| 7 | g12834.t1 | SUPERFAMILY | SSF56104 | SAICAR synthase-like | 22 | 254 | 6.54E-65 |
| 8 | g12834.t1 | SUPERFAMILY | SSF52255 | N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE) | 268 | 423 | 1.27E-36 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004639 | phosphoribosylaminoimidazolesuccinocarboxamide synthase activity | MF |
| GO:0006164 | purine nucleotide biosynthetic process | BP |
| GO:0006189 | ‘de novo’ IMP biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01587 (phosphoribosylaminoimidazole carboxylase / phosphoribosylaminoimidazole-succinocarboxamide synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
