General information for g12855.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heparan-alpha-glucosaminide N-acetyltransferase.
Parent gene
- The parent gene g12855 is located on chr_1: 26570404..26571213 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12855 | g12855.t1 | TTS | g12855.t1 | 26569336 | 26569336 |
| chr_1 | g12855 | g12855.t1 | isoform | g12855.t1 | 26569421 | 26571213 |
| chr_1 | g12855 | g12855.t1 | exon | g12855.t1.exon1 | 26569421 | 26570353 |
| chr_1 | g12855 | g12855.t1 | cds | g12855.t1.CDS1 | 26569421 | 26570353 |
| chr_1 | g12855 | g12855.t1 | exon | g12855.t1.exon2 | 26570404 | 26570765 |
| chr_1 | g12855 | g12855.t1 | cds | g12855.t1.CDS2 | 26570404 | 26570765 |
| chr_1 | g12855 | g12855.t1 | exon | g12855.t1.exon3 | 26570820 | 26571213 |
| chr_1 | g12855 | g12855.t1 | cds | g12855.t1.CDS3 | 26570820 | 26571213 |
| chr_1 | g12855 | g12855.t1 | TSS | g12855.t1 | 26571224 | 26571224 |
Sequences
>g12855.t1 Gene=g12855 Length=1689
ATGTCGTGGCTTGAACGCACTGACAGTCATTTTCGTGATTTAAATTTAAAAAGTTTGTGG
GTTGATGAAGCTTTTCTAAATATCAGTAATGGCATGAATTTGCCATTGTATTTGTATACA
CTTTCACCAGACTGTGTTAAATGTCCATTTCGTAAATTTAATGAGATTGCAGCAAATAAT
GACACAGTGATGAAAATAAAAGTTGCAAGAACACCACAAATGAAACTTTATTATAAAGAT
CATGAAAAATATATTTTCAATAATCAATCAATGGATGGAATTTATGCACATATTGAACCT
AAAATGGGACAATTTGGTGTTTATGATTGGATAATTAATGAAAATGAAGACAATGACTTT
GAAGTTGCTAAAGAACCAGTACCAATTTATTGGTCCATGCTTGTTGTTGCTGTAATCGTT
TTTGCATTCTATTGGTTCTGTAAAGGAGTTGATCGATTAATTTTATCTCGCTTTAAAAAC
ACAAACAAGAACACATCGATAAATGGACCACAAGTGAAAAAACGATTGAAAAGTTTAGAT
ATTTTTCGAGGGATTTCGATTGTTTTGATGATTTTTGTATGGTATGGTGCTGGTTTTTAT
TGGTGGTTGCAACATGCTGTTTGGAATGGACTGTTAGCTGCTGACATTGTTTTTCCATGG
TTCTTATTCATAATGGGCGTTTGCATACCAATTTCTATTAAATCACAACTCTCTAAGAAT
TTTGCGAAAATTAAAATCATTAAAAAAATTATCAAGAGGTCAATTCTACTTTTCCTAATT
GGACTTTGTATTTTTGCACAAGGAGTTATTTTCCGTGAAGTCAGAATCATGAACGTTTTG
CAGCGTTTTGCAATTTCTTATCTTGTCGTGTGTTTAGTTCATGTTGCATTTACAAAAACG
ACTGAAGTCAAAACACCTCAATTATTAGATGATATTTTGATTATTTGGAAAGAATGGATA
GTTATCCTTGTAATTCTTGTAATTCATCTTGCAACAGTTTTTGGAATAAAAATAGATGGC
TGTGATCGTGGATATGTTGGTCCAGGAGGAATTCATGATGATATGAAAAATGCGTTATGC
ACTGGTGGACTTGTTCAATATATTGATGAAAAAATTTTAACTGAAAATCATTTATTTCGT
TGGGCTGATATCAGATCAATTTATGATGCTACAAAAAGATTCGATCCTGAAGGACTTTAC
GGTTCATTGACATCAATTGTACATACAATGATAGGCTTTCAATGTGGAATGACATTGATG
ACTTTTACAGGACATAAACAAAGAATCATTAGATGGGTAATATGGGGCGCATTTATGGCA
ATATTGACAGCAATTCTAACATTTTGCAAAATTGAAGATGCACCAATTCCAATTAACAAA
CAAATGTGGTCGCTTTCATTTGTTACACTAACATCAGCAACATCGTTTTGGTTGCTTGCT
CTAATTTATTTCCTTGTTGACGTGAAAAATTATTGCGAAGATTTTTGGACAATTTTTCAA
TATCCTGGAATGAATGCGATAATTTTGTACATGGGAAATGCTATTTTTAGCTCACATTGG
CCATTTCATTTCTTTGTAGCTGACATGAATACACATTTTATATATTTACTGAAACATTGC
TATACGGCTGCAATTTGGATTCTCGCAGCTCACATCATGTACCATAAAAAGTTTTTCTTT
AATCTTTAA
>g12855.t1 Gene=g12855 Length=562
MSWLERTDSHFRDLNLKSLWVDEAFLNISNGMNLPLYLYTLSPDCVKCPFRKFNEIAANN
DTVMKIKVARTPQMKLYYKDHEKYIFNNQSMDGIYAHIEPKMGQFGVYDWIINENEDNDF
EVAKEPVPIYWSMLVVAVIVFAFYWFCKGVDRLILSRFKNTNKNTSINGPQVKKRLKSLD
IFRGISIVLMIFVWYGAGFYWWLQHAVWNGLLAADIVFPWFLFIMGVCIPISIKSQLSKN
FAKIKIIKKIIKRSILLFLIGLCIFAQGVIFREVRIMNVLQRFAISYLVVCLVHVAFTKT
TEVKTPQLLDDILIIWKEWIVILVILVIHLATVFGIKIDGCDRGYVGPGGIHDDMKNALC
TGGLVQYIDEKILTENHLFRWADIRSIYDATKRFDPEGLYGSLTSIVHTMIGFQCGMTLM
TFTGHKQRIIRWVIWGAFMAILTAILTFCKIEDAPIPINKQMWSLSFVTLTSATSFWLLA
LIYFLVDVKNYCEDFWTIFQYPGMNAIILYMGNAIFSSHWPFHFFVADMNTHFIYLLKHC
YTAAIWILAAHIMYHKKFFFNL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g12855.t1 | PANTHER | PTHR31061 | LD22376P | 153 | 558 | 1.9E-107 |
| 3 | g12855.t1 | PANTHER | PTHR31061:SF19 | HEPARAN-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE | 153 | 558 | 1.9E-107 |
| 1 | g12855.t1 | Pfam | PF16401 | Domain of unknown function (DUF5009) | 177 | 304 | 9.8E-7 |
| 23 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 128 | - |
| 28 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 129 | 147 | - |
| 16 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 148 | 180 | - |
| 32 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 181 | 202 | - |
| 26 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 203 | 207 | - |
| 29 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 208 | 233 | - |
| 17 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 234 | 253 | - |
| 34 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 254 | 274 | - |
| 25 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 275 | 279 | - |
| 35 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 280 | 298 | - |
| 20 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 299 | 318 | - |
| 36 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 319 | 338 | - |
| 24 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 339 | 398 | - |
| 31 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 420 | - |
| 19 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 421 | 431 | - |
| 37 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 432 | 451 | - |
| 22 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 452 | 462 | - |
| 27 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 463 | 486 | - |
| 18 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 487 | 506 | - |
| 33 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 507 | 527 | - |
| 21 | g12855.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 528 | 532 | - |
| 30 | g12855.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 533 | 554 | - |
| 15 | g12855.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 555 | 562 | - |
| 8 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 128 | 147 | - |
| 4 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 181 | 203 | - |
| 5 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 216 | 233 | - |
| 9 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 254 | 271 | - |
| 13 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 276 | 298 | - |
| 11 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 319 | 336 | - |
| 14 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 400 | 422 | - |
| 12 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 429 | 448 | - |
| 6 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 463 | 485 | - |
| 10 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 498 | 517 | - |
| 7 | g12855.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 532 | 554 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005765 | lysosomal membrane | CC |
| GO:0015019 | heparan-alpha-glucosaminide N-acetyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K10532 (heparan-alpha-glucosaminide N-acetyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed