Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable cytochrome P450 6a13.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g12864 g12864.t5 TTS g12864.t5 26631534 26631534
chr_1 g12864 g12864.t5 isoform g12864.t5 26631566 26633456
chr_1 g12864 g12864.t5 exon g12864.t5.exon1 26631566 26632517
chr_1 g12864 g12864.t5 cds g12864.t5.CDS1 26631715 26632517
chr_1 g12864 g12864.t5 exon g12864.t5.exon2 26632817 26633049
chr_1 g12864 g12864.t5 cds g12864.t5.CDS2 26632817 26633049
chr_1 g12864 g12864.t5 exon g12864.t5.exon3 26633107 26633456
chr_1 g12864 g12864.t5 cds g12864.t5.CDS3 26633107 26633456
chr_1 g12864 g12864.t5 TSS g12864.t5 26633479 26633479

Sequences

>g12864.t5 Gene=g12864 Length=1535
ATGAGCACAACTTTAGTAGCATTGATCATCATTTTATTTAGTGTTGCTTACATTTTTGTA
AAGCGACAATTTAGTTATTGGTCACGAAGAGGATTTTTTCAAGCTGACGCTTCGTTTCCT
TTTGGATCAATTAAAGGAGCAAATTTTTCTATAACATTGACTGAAAAATTGGATGAAATT
TATAAACAATTCAAAGGAAAAGCTTCTGTCGTAGGTTTTTATAATTTCTTAGGAGCATCA
ATTTTACCTATAGATCCAGAAATTATAAAACACATTTTTGTCAAAGATTTTGCTTCATTT
CATGATCGAGGATTTTTTTATAATAAAAGAGATGATCCGCTTACTAATAACATTCTTTCT
ATCAAAGGTCAAGAATGGAAAGATCGTAGGGCTAAATTCACTCCTGTATTTACTTCAGCT
AAAATGAAGCAAATGTATGAAATTATTGAAGAAATTAGTGACAAATTTGCTCTTAAACTC
GGTGAAAGTTTAAAAGACTCAAATATTCAAGACATGAGAATTTGGGCACGAAGATTTACA
AATGATAATATTGGTAGTACTGTGTTTGGAATTGACGCAAAATGTCTTGAAGATATAAAT
TCTGATTTGATGGTGTTTGGTCAAAAATCCCTTAACATGACACTTGCGGGTGCATTTAAA
TTTGCATTTGGTTCAACTTTTCCTGAAATTGCAAGATTTTTACGATTTAAAATTCTTGAC
AAAGATTCACATGAGTTTTTCCTAAATACATTTCTTCAAACTTTAGAATACCGAAGAAAT
AATAAGCAAATTGTTCGTCATGATTTTGTGTCAATGCTGTTAGAATTATCGGACCAATTA
CCACCAATTGATCTTGCTGCTGAAGCCACAATAATGTATTTAGCTGGTTTTGAAACAAGT
GCAACTTTGATTTCTTTTGCTTTGTATGAATTGGCTTTAAACTCAGAAATGCAAGATCGA
TTGAGAGATGAAATTTTAAATGGAATAAATGAAAATGATGGAAAAATTACTTATGAAATG
CTTTATGGATTTGAATATCTTGACATGGTTATAAACGAAACTTTAAGAAAACTTCCATCA
ATTCCACACATTTTTAGAAAATGCACAAAAGATTTTGTAGTGCCAAATACGAATCTTGTT
ATTCCAAAAGGAACTCAAGTTATTATTAATAATTACTCACTACAACATGATCCAGAATAT
TTTCCAAATCCTGAAAAATTTGATCCAGAAAGATTCAACAGTGAAAATAAAAAGAAAATT
AATCCGTATACAAATATGCCATTTGCACTTGGTAATCGTAATTGTTTGGTAAGAATTTTC
ACTTTTATTAAATTCAATTTATGTGTAAAGTTTTCTAATTTTTATAGGGCGAGCGCTTTG
GATTAATGCAAGCTAAAATTGGCGTAGTAAAAATGATTAAAAATTTTATTTTTACACCAT
GTAATAAAACTACTATTCCAATGAAATTTGTTCCTAATCAATTTTTCTTAACTCCAGTTG
GTGGAATTCAATTATGCATCAAAAAAATTCATTAA

>g12864.t5 Gene=g12864 Length=461
MSTTLVALIIILFSVAYIFVKRQFSYWSRRGFFQADASFPFGSIKGANFSITLTEKLDEI
YKQFKGKASVVGFYNFLGASILPIDPEIIKHIFVKDFASFHDRGFFYNKRDDPLTNNILS
IKGQEWKDRRAKFTPVFTSAKMKQMYEIIEEISDKFALKLGESLKDSNIQDMRIWARRFT
NDNIGSTVFGIDAKCLEDINSDLMVFGQKSLNMTLAGAFKFAFGSTFPEIARFLRFKILD
KDSHEFFLNTFLQTLEYRRNNKQIVRHDFVSMLLELSDQLPPIDLAAEATIMYLAGFETS
ATLISFALYELALNSEMQDRLRDEILNGINENDGKITYEMLYGFEYLDMVINETLRKLPS
IPHIFRKCTKDFVVPNTNLVIPKGTQVIINNYSLQHDPEYFPNPEKFDPERFNSENKKKI
NPYTNMPFALGNRNCLVRIFTFIKFNLCVKFSNFYRASALD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g12864.t5 Gene3D G3DSA:1.10.630.10 Cytochrome p450 15 449 9.1E-108
2 g12864.t5 PANTHER PTHR24292 CYTOCHROME P450 7 444 3.6E-134
5 g12864.t5 PRINTS PR00463 E-class P450 group I signature 284 301 3.6E-16
10 g12864.t5 PRINTS PR00385 P450 superfamily signature 295 312 1.9E-8
7 g12864.t5 PRINTS PR00463 E-class P450 group I signature 304 330 3.6E-16
4 g12864.t5 PRINTS PR00463 E-class P450 group I signature 348 366 3.6E-16
9 g12864.t5 PRINTS PR00385 P450 superfamily signature 349 360 1.9E-8
6 g12864.t5 PRINTS PR00463 E-class P450 group I signature 390 414 3.6E-16
3 g12864.t5 PRINTS PR00463 E-class P450 group I signature 425 435 3.6E-16
8 g12864.t5 PRINTS PR00385 P450 superfamily signature 426 435 1.9E-8
1 g12864.t5 Pfam PF00067 Cytochrome P450 49 436 3.1E-76
15 g12864.t5 Phobius SIGNAL_PEPTIDE Signal peptide region 1 16 -
16 g12864.t5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 3 -
17 g12864.t5 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 4 12 -
18 g12864.t5 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 13 16 -
14 g12864.t5 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 17 461 -
12 g12864.t5 SUPERFAMILY SSF48264 Cytochrome P450 55 436 4.98E-93
11 g12864.t5 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 5 27 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed