General information for g12991.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aspartate aminotransferase, cytoplasmic.
Parent gene
- The parent gene g12991 is located on chr_1: 27385450..27386700 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12991 | g12991.t1 | TSS | g12991.t1 | 27384347 | 27384347 |
| chr_1 | g12991 | g12991.t1 | isoform | g12991.t1 | 27384408 | 27386348 |
| chr_1 | g12991 | g12991.t1 | exon | g12991.t1.exon1 | 27384408 | 27384519 |
| chr_1 | g12991 | g12991.t1 | cds | g12991.t1.CDS1 | 27384408 | 27384519 |
| chr_1 | g12991 | g12991.t1 | exon | g12991.t1.exon2 | 27385237 | 27386348 |
| chr_1 | g12991 | g12991.t1 | cds | g12991.t1.CDS2 | 27385237 | 27386348 |
| chr_1 | g12991 | g12991.t1 | TTS | g12991.t1 | 27386632 | 27386632 |
Sequences
>g12991.t1 Gene=g12991 Length=1224
ATGGCAAGCATTTTCGATGGCATCGAGAAAGGACCTCCAATTGAAGTATTTGCATTAAAT
AAACTCTTCCTTGAAGACACTGATAGCAGAAAAGTCAATCTTGGTGTCGGAGCCTATCGT
ACGGAAGAAGGAAAACCTTGGGTTTTGCCAGTTGTAAAAAAGGCAGAAAAGGCAGTTGTC
GATGATGACTCTTTGAATCATGAGTATCTTCCAGTGCTTGGTCTAGATACTTATGCATCA
GCAGCAACTGCACTTTTGCTCGGTGAAGACTCAATTCAAATTAAAAACAAAAATGCATTT
GGTATTCAAACATTGTCTGGTACAGGTGCTTTACGTTTGGGAGCTGAATTTCTTGCTCGT
GTAATGGGCAGGAGAGTTTTTTACTACTCAGAACCTACATGGGAAAATCATCAAAAAGTT
TTTCTTAGTGCTGGTTTTACTGATGGACATGCTTATCGTTACTGGGATCCTACAACTCGT
GGTATTGATTTTGATGGATTGATTGCCGATTTAAAAAAAGCTCCTGAAGGTGCTGTAATC
ATTTTACATGCTTGTGCTCATAATCCAACTGGATGTGATCCAACAAAAGAGCAATGGGTT
AAAATTGCTGATATTATTGAAGAAAAGAAACTTTTCCCCTTCTTTGATTCGGCCTATCAA
GGCTTTGCAAGTGGAGATCCAGTTCATGATGCTTTCGCTGTTCGTTATTTTGTTGAACGC
GGTTTCGAAATTTTCTGCTCACAATCATTTGCCAAAAATTTTGGTTTGTATAATGAACGT
ATTGGAAACTTGACTGTGGTTCAAAAAAATCCAGCAACTTCAGCAGCAATTCAATCACGA
ATGACTTTGCTCGTGAGATTTATGTATTCGAACCCACCAGCTTTTGGTGGTCGAATTGTT
GGAAAAGTACTCAACTCACCAGAATTGCGTCAAGAATGGATGGACTCTATTAAAATTATG
TCATCGAGAATCATTAAAATGCGTAAATTGTTGAGAGAGGAATTGGAAAACTTAAAAACA
CCAGGAACATGGGAGCACATAACACAACAAATTGGAATGTTCTCTTATACAGGATTAACT
GAAGCACAAGTAAAGATTCTCATTGAAAAATATCACATTTACTTACTTGCAAGTGGACGT
ATTAATATGTGTGGTCTAAATGAGAAAAATTACAAATATGTTGCGCAATCAATTTATGAT
GCCATACAAGCCACTAAGGTATAA
>g12991.t1 Gene=g12991 Length=407
MASIFDGIEKGPPIEVFALNKLFLEDTDSRKVNLGVGAYRTEEGKPWVLPVVKKAEKAVV
DDDSLNHEYLPVLGLDTYASAATALLLGEDSIQIKNKNAFGIQTLSGTGALRLGAEFLAR
VMGRRVFYYSEPTWENHQKVFLSAGFTDGHAYRYWDPTTRGIDFDGLIADLKKAPEGAVI
ILHACAHNPTGCDPTKEQWVKIADIIEEKKLFPFFDSAYQGFASGDPVHDAFAVRYFVER
GFEIFCSQSFAKNFGLYNERIGNLTVVQKNPATSAAIQSRMTLLVRFMYSNPPAFGGRIV
GKVLNSPELRQEWMDSIKIMSSRIIKMRKLLREELENLKTPGTWEHITQQIGMFSYTGLT
EAQVKILIEKYHIYLLASGRINMCGLNEKNYKYVAQSIYDAIQATKV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g12991.t1 | CDD | cd00609 | AAT_like | 32 | 390 | 4.24612E-47 |
| 9 | g12991.t1 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 8 | 398 | 1.7E-171 |
| 10 | g12991.t1 | Gene3D | G3DSA:3.40.640.10 | - | 64 | 306 | 1.7E-171 |
| 2 | g12991.t1 | PANTHER | PTHR11879 | ASPARTATE AMINOTRANSFERASE | 2 | 402 | 1.4E-173 |
| 6 | g12991.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 180 | 199 | 4.1E-34 |
| 5 | g12991.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 211 | 223 | 4.1E-34 |
| 4 | g12991.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 279 | 304 | 4.1E-34 |
| 3 | g12991.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 347 | 365 | 4.1E-34 |
| 1 | g12991.t1 | Pfam | PF00155 | Aminotransferase class I and II | 30 | 398 | 4.0E-87 |
| 8 | g12991.t1 | ProSitePatterns | PS00105 | Aminotransferases class-I pyridoxal-phosphate attachment site. | 249 | 262 | - |
| 7 | g12991.t1 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 1 | 403 | 1.79E-116 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009058 | biosynthetic process | BP |
| GO:0006520 | cellular amino acid metabolic process | BP |
| GO:0008483 | transaminase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
KEGG
Orthology
- This transcript has been annotated with K14454 (aspartate aminotransferase, cytoplasmic)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
