General information for g12991.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aspartate aminotransferase, cytoplasmic.
Parent gene
- The parent gene g12991 is located on chr_1: 27385450..27386700 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12991 | g12991.t3 | TSS | g12991.t3 | 27384347 | 27384347 |
| chr_1 | g12991 | g12991.t3 | isoform | g12991.t3 | 27384408 | 27386348 |
| chr_1 | g12991 | g12991.t3 | exon | g12991.t3.exon1 | 27384408 | 27384519 |
| chr_1 | g12991 | g12991.t3 | cds | g12991.t3.CDS1 | 27384408 | 27384519 |
| chr_1 | g12991 | g12991.t3 | exon | g12991.t3.exon2 | 27385237 | 27385386 |
| chr_1 | g12991 | g12991.t3 | cds | g12991.t3.CDS2 | 27385237 | 27385386 |
| chr_1 | g12991 | g12991.t3 | exon | g12991.t3.exon3 | 27385450 | 27386348 |
| chr_1 | g12991 | g12991.t3 | cds | g12991.t3.CDS3 | 27385450 | 27386348 |
| chr_1 | g12991 | g12991.t3 | TTS | g12991.t3 | 27386632 | 27386632 |
Sequences
>g12991.t3 Gene=g12991 Length=1161
ATGGCAAGCATTTTCGATGGCATCGAGAAAGGACCTCCAATTGAAGTATTTGCATTAAAT
AAACTCTTCCTTGAAGACACTGATAGCAGAAAAGTCAATCTTGGTGTCGGAGCCTATCGT
ACGGAAGAAGGAAAACCTTGGGTTTTGCCAGTTGTAAAAAAGGCAGAAAAGGCAGTTGTC
GATGATGACTCTTTGAATCATGAGTATCTTCCAGTGCTTGGTCTAGATACTTATGCATCA
GCAGCAACTGCACTTTTGCTCGGTGCTTTACGTTTGGGAGCTGAATTTCTTGCTCGTGTA
ATGGGCAGGAGAGTTTTTTACTACTCAGAACCTACATGGGAAAATCATCAAAAAGTTTTT
CTTAGTGCTGGTTTTACTGATGGACATGCTTATCGTTACTGGGATCCTACAACTCGTGGT
ATTGATTTTGATGGATTGATTGCCGATTTAAAAAAAGCTCCTGAAGGTGCTGTAATCATT
TTACATGCTTGTGCTCATAATCCAACTGGATGTGATCCAACAAAAGAGCAATGGGTTAAA
ATTGCTGATATTATTGAAGAAAAGAAACTTTTCCCCTTCTTTGATTCGGCCTATCAAGGC
TTTGCAAGTGGAGATCCAGTTCATGATGCTTTCGCTGTTCGTTATTTTGTTGAACGCGGT
TTCGAAATTTTCTGCTCACAATCATTTGCCAAAAATTTTGGTTTGTATAATGAACGTATT
GGAAACTTGACTGTGGTTCAAAAAAATCCAGCAACTTCAGCAGCAATTCAATCACGAATG
ACTTTGCTCGTGAGATTTATGTATTCGAACCCACCAGCTTTTGGTGGTCGAATTGTTGGA
AAAGTACTCAACTCACCAGAATTGCGTCAAGAATGGATGGACTCTATTAAAATTATGTCA
TCGAGAATCATTAAAATGCGTAAATTGTTGAGAGAGGAATTGGAAAACTTAAAAACACCA
GGAACATGGGAGCACATAACACAACAAATTGGAATGTTCTCTTATACAGGATTAACTGAA
GCACAAGTAAAGATTCTCATTGAAAAATATCACATTTACTTACTTGCAAGTGGACGTATT
AATATGTGTGGTCTAAATGAGAAAAATTACAAATATGTTGCGCAATCAATTTATGATGCC
ATACAAGCCACTAAGGTATAA
>g12991.t3 Gene=g12991 Length=386
MASIFDGIEKGPPIEVFALNKLFLEDTDSRKVNLGVGAYRTEEGKPWVLPVVKKAEKAVV
DDDSLNHEYLPVLGLDTYASAATALLLGALRLGAEFLARVMGRRVFYYSEPTWENHQKVF
LSAGFTDGHAYRYWDPTTRGIDFDGLIADLKKAPEGAVIILHACAHNPTGCDPTKEQWVK
IADIIEEKKLFPFFDSAYQGFASGDPVHDAFAVRYFVERGFEIFCSQSFAKNFGLYNERI
GNLTVVQKNPATSAAIQSRMTLLVRFMYSNPPAFGGRIVGKVLNSPELRQEWMDSIKIMS
SRIIKMRKLLREELENLKTPGTWEHITQQIGMFSYTGLTEAQVKILIEKYHIYLLASGRI
NMCGLNEKNYKYVAQSIYDAIQATKV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g12991.t3 | CDD | cd00609 | AAT_like | 32 | 369 | 5.08597E-37 |
| 9 | g12991.t3 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 22 | 377 | 7.6E-153 |
| 10 | g12991.t3 | Gene3D | G3DSA:3.40.640.10 | - | 48 | 300 | 7.6E-153 |
| 2 | g12991.t3 | PANTHER | PTHR11879 | ASPARTATE AMINOTRANSFERASE | 2 | 381 | 1.1E-158 |
| 4 | g12991.t3 | PRINTS | PR00799 | Aspartate aminotransferase signature | 159 | 178 | 3.4E-34 |
| 6 | g12991.t3 | PRINTS | PR00799 | Aspartate aminotransferase signature | 190 | 202 | 3.4E-34 |
| 5 | g12991.t3 | PRINTS | PR00799 | Aspartate aminotransferase signature | 258 | 283 | 3.4E-34 |
| 3 | g12991.t3 | PRINTS | PR00799 | Aspartate aminotransferase signature | 326 | 344 | 3.4E-34 |
| 1 | g12991.t3 | Pfam | PF00155 | Aminotransferase class I and II | 30 | 377 | 1.9E-77 |
| 8 | g12991.t3 | ProSitePatterns | PS00105 | Aminotransferases class-I pyridoxal-phosphate attachment site. | 228 | 241 | - |
| 7 | g12991.t3 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 1 | 382 | 1.12E-107 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009058 | biosynthetic process | BP |
| GO:0006520 | cellular amino acid metabolic process | BP |
| GO:0008483 | transaminase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
KEGG
Orthology
- This transcript has been annotated with K14454 (aspartate aminotransferase, cytoplasmic)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
