Gene loci information

Transcript annotation

  • This transcript has been annotated as Vinculin.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13030 g13030.t4 isoform g13030.t4 27594573 27595760
chr_1 g13030 g13030.t4 exon g13030.t4.exon1 27594573 27595564
chr_1 g13030 g13030.t4 cds g13030.t4.CDS1 27594575 27595105
chr_1 g13030 g13030.t4 exon g13030.t4.exon2 27595621 27595760
chr_1 g13030 g13030.t4 TSS g13030.t4 NA NA
chr_1 g13030 g13030.t4 TTS g13030.t4 NA NA

Sequences

>g13030.t4 Gene=g13030 Length=1132
GTCAACAAAGTAAAGGAACATCACCACAAGCTGAATCATTGGCTCGTGAAATGATTGACA
AATTGGATGAATTAGATCAAAATATTTTACAAGCTGTTATTAATGTTGATAAAGCTGGTC
TTCAACAAACTGCGCATACTGTTCAAGGACGTTTAGAACAAGCAATTCGTTGGTTAACAA
ATCCTGGGGCTGATGACAAAGGCTTGGGTCAACGTGCTATTGCACTTATCATTGAAGAAG
GAAAGAAAGTTGCCGATGGCTTGCCAGGACATCAAAAGTCCGATATTCTCTATCTTTGTG
ATGAAGTAGAAAATCTTGCCAATTAATATTCAAAATTGTGTTCAGCGGGCTTGGCTCACA
CACCCGAAGCTCAAGAAATTGCTCGCAAATTAAATGCAAAATTGCATGAGTTGAAGAATT
TGATTCAATCAGCCGTTGTTGGTCGTGTAGTTGAAGATTTTATCGATATTTCAACGCCAT
TGAAACAATTTACTGAAGCTGTCAATGCACCAGAAGGAACACCGAATCGCGAACAAAATT
TCAACCAAAAGGCGGCAAATTTGCAAGCATTCAGTGACCGTGCATCAAAGACAAGTCGAA
TGGTTGCTGCTGGTGGTTCAGGTGGAAACAAAAAACTCGCTGAAGTTCTTCTTGCATCAG
CTGCACAAATTGATAGTCTTACACCACAACTTATTAGTGCTGGTCGTATTCGTATGAATT
ATCCAGGATCAAAGGCTGCTGAAGAACATTATAATAATCTTAAGCAACAATATGCTGACA
CTATTTTGAGAATGAGAACTCTCTGCGATCAAGCAACTGATCCAGCTGATTTCATCAAAG
CCAGTGAAGAACAAATGCAAAAACACACATTATTGTGCGAAGATGCAATTAGAAATCGAG
TCCCACAAAAAATGGTTGACAACACAAGCAGCATTGCACGTTTGGCCAATCGTGTATTGT
TAGTTGCCAAACAAGAAGCTGACAATTCTGAAGATCCACAATTTACCTCAGAATTGCTTA
ATGCTTCCGATAAGCTTCAAAATTCAGTTCCGACAATGGTACAAAATGCTAAATCTGTTG
CAACTGACATTACGAATGGGGCTGCTGGTGCTAATTGGCGTGATGCGAATAA

>g13030.t4 Gene=g13030 Length=177
MVAAGGSGGNKKLAEVLLASAAQIDSLTPQLISAGRIRMNYPGSKAAEEHYNNLKQQYAD
TILRMRTLCDQATDPADFIKASEEQMQKHTLLCEDAIRNRVPQKMVDNTSSIARLANRVL
LVAKQEADNSEDPQFTSELLNASDKLQNSVPTMVQNAKSVATDITNGAAGANWRDAN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g13030.t4 Coils Coil Coil 79 99 -
6 g13030.t4 Gene3D G3DSA:1.20.120.810 - 1 73 7.8E-19
7 g13030.t4 Gene3D G3DSA:1.20.120.230 - 76 177 7.6E-41
2 g13030.t4 PANTHER PTHR46180:SF1 VINCULIN 1 176 2.4E-56
3 g13030.t4 PANTHER PTHR46180 VINCULIN 1 176 2.4E-56
1 g13030.t4 Pfam PF01044 Vinculin family 2 176 1.0E-62
4 g13030.t4 SUPERFAMILY SSF47220 alpha-catenin/vinculin-like 4 74 1.73E-18
5 g13030.t4 SUPERFAMILY SSF47220 alpha-catenin/vinculin-like 76 177 9.42E-34

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0051015 actin filament binding MF
GO:0003779 actin binding MF
GO:0007155 cell adhesion BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values