Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13107 g13107.t21 TTS g13107.t21 27991332 27991332
chr_1 g13107 g13107.t21 isoform g13107.t21 27991463 27992591
chr_1 g13107 g13107.t21 exon g13107.t21.exon1 27991463 27991985
chr_1 g13107 g13107.t21 cds g13107.t21.CDS1 27991464 27991985
chr_1 g13107 g13107.t21 exon g13107.t21.exon2 27992042 27992208
chr_1 g13107 g13107.t21 cds g13107.t21.CDS2 27992042 27992208
chr_1 g13107 g13107.t21 exon g13107.t21.exon3 27992322 27992354
chr_1 g13107 g13107.t21 cds g13107.t21.CDS3 27992322 27992354
chr_1 g13107 g13107.t21 exon g13107.t21.exon4 27992413 27992591
chr_1 g13107 g13107.t21 cds g13107.t21.CDS4 27992413 27992581
chr_1 g13107 g13107.t21 TSS g13107.t21 27993083 27993083

Sequences

>g13107.t21 Gene=g13107 Length=902
AATTTCCGGCATGTTTGAATTCAATCGTCCAGCATATTTTATTCGTGACCCAGAGATGAT
TAAACGACTTTGTATTAAAGAATTTGATTCATTCAGCGATCATCGACTTATTCTTTCTAA
AGAAGCAGAACCTCTCTTTGCTTCTGCACTTTTCGGTTTGACGGGTCAAAAATGGAGAGA
CATGCGAGCAACACTCTCTCCAGCGTTTACTGGCAGCAAAATGCGTCTAATGTTTAAGCT
CATAAACGACGTTGGCTCTCAAATGTCGAATACTGTTCTTGAACAAATAAATAAAGGAGC
TGACAACAAAATTGAGTTTAAAGAATTTGCTCAGAAATTTACAATCGATATTATAGCAAG
TTGCGCATTTGGATTAGAGATAAATTCATTCAATAATCCCAATAATGACTTCCTGAGAAT
TGCAAGAAAAGCAACAAATTTCGCTACCTTTAAGACCACAATAAAATTAGTGGGTTTACT
TGGTGCACCATGGTTAATGAAAATATTTAAAATCAAGTTTTTGGATGAAGAATTTTATAA
ATTCTTTGGTGCAATGATGACAGATACTATAAAAACTAGAGAAGAGAAGAAAATAACTCG
TAATGATATGATCGATTTGATATTGCAAGCAAAACACGGTAAACAACTTGATTATGACAA
TGAAGAGCATACAGTTGATGGTTTCGCAACAGTTGAGGAATCACAATTAGGAAAAGAAAA
AGTTAAGAGAACATGGGAAGATGATGAACTTTTAGCACAATGTGTTGGCTTCTTTTTTGG
TGGATTTGAAACAGTTTCGAGTGTCATGACTTTTATGGCTGCTGAATTGATTATTGATCC
TAATATTCAGAAAAAATTACAAGACGAAATTGATGAAGTTCACAAGTCATTGAACGGAGA
AA

>g13107.t21 Gene=g13107 Length=297
MFEFNRPAYFIRDPEMIKRLCIKEFDSFSDHRLILSKEAEPLFASALFGLTGQKWRDMRA
TLSPAFTGSKMRLMFKLINDVGSQMSNTVLEQINKGADNKIEFKEFAQKFTIDIIASCAF
GLEINSFNNPNNDFLRIARKATNFATFKTTIKLVGLLGAPWLMKIFKIKFLDEEFYKFFG
AMMTDTIKTREEKKITRNDMIDLILQAKHGKQLDYDNEEHTVDGFATVEESQLGKEKVKR
TWEDDELLAQCVGFFFGGFETVSSVMTFMAAELIIDPNIQKKLQDEIDEVHKSLNGE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g13107.t21 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 296 0
2 g13107.t21 PANTHER PTHR24292 CYTOCHROME P450 1 295 0
3 g13107.t21 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 1 295 0
5 g13107.t21 PRINTS PR00464 Group II E-class P450 signature 52 72 0
4 g13107.t21 PRINTS PR00464 Group II E-class P450 signature 109 127 0
1 g13107.t21 Pfam PF00067 Cytochrome P450 6 291 0
6 g13107.t21 SUPERFAMILY SSF48264 Cytochrome P450 4 293 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values