Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 9f2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13107 g13107.t32 TTS g13107.t32 27991332 27991332
chr_1 g13107 g13107.t32 isoform g13107.t32 27991917 27993039
chr_1 g13107 g13107.t32 exon g13107.t32.exon1 27991917 27991985
chr_1 g13107 g13107.t32 cds g13107.t32.CDS1 27991917 27991985
chr_1 g13107 g13107.t32 exon g13107.t32.exon2 27992042 27992220
chr_1 g13107 g13107.t32 cds g13107.t32.CDS2 27992042 27992220
chr_1 g13107 g13107.t32 exon g13107.t32.exon3 27992413 27992591
chr_1 g13107 g13107.t32 cds g13107.t32.CDS3 27992413 27992591
chr_1 g13107 g13107.t32 exon g13107.t32.exon4 27992658 27992885
chr_1 g13107 g13107.t32 cds g13107.t32.CDS4 27992658 27992860
chr_1 g13107 g13107.t32 exon g13107.t32.exon5 27993023 27993039
chr_1 g13107 g13107.t32 TSS g13107.t32 27993083 27993083

Sequences

>g13107.t32 Gene=g13107 Length=672
GCTGAAGTTAATATCAAGTCATTCTAATTCCAGTTTGTCATAATGATTATTTGGATTTTA
TTATTGATTGCCGCAGTCATTTTGCTGTACAAATATGGAACGAGAAATTTCGATTACTTT
CAAAAGAAAGGACTGGCTTTTAATAAGCCTCATTTTCTTGTTGGGTCACGCCTAAGTACA
GTTCTTGGTACAAAAAATATGATCGAAATGCTCACTGAAATTTATAATGAGCATAGAGAT
GAAAAAATTTCCGGCATGTTTGAATTCAATCGTCCAGCATATTTTATTCGTGACCCAGAG
ATGATTAAACGACTTTGTATTAAAGAATTTGATTCATTCAGCGATCATCGACTTATTCTT
TCTAAAGAAGCAGAACCTCTCTTTGCTTCTGCACTTTTCGGTTTGACGGGTCAAAAATGG
AGAGCATACACTTTAGGCAGCAAAATGCGTCTAATGTTTAAGCTCATAAACGACGTTGGC
TCTCAAATGTCGAATACTGTTCTTGAACAAATAAATAAAGGAGCTGACAACAAAATTGAG
TTTAAAGAATTTGCTCAGAAATTTACAATCGATATTATAGCAAGTTGCGCATTTGGATTA
GAGATAAATTCATTCAATAATCCCAATAATGACTTCCTGAGAATTGCAAGAAAAGCAACA
AATTTCGCTACC

>g13107.t32 Gene=g13107 Length=210
MIIWILLLIAAVILLYKYGTRNFDYFQKKGLAFNKPHFLVGSRLSTVLGTKNMIEMLTEI
YNEHRDEKISGMFEFNRPAYFIRDPEMIKRLCIKEFDSFSDHRLILSKEAEPLFASALFG
LTGQKWRAYTLGSKMRLMFKLINDVGSQMSNTVLEQINKGADNKIEFKEFAQKFTIDIIA
SCAFGLEINSFNNPNNDFLRIARKATNFAT

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g13107.t32 Gene3D G3DSA:1.10.630.10 Cytochrome p450 14 210 3.5E-31
2 g13107.t32 PANTHER PTHR24292 CYTOCHROME P450 5 208 5.8E-45
1 g13107.t32 Pfam PF00067 Cytochrome P450 35 201 3.3E-12
7 g13107.t32 Phobius SIGNAL_PEPTIDE Signal peptide region 1 19 -
8 g13107.t32 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 3 -
9 g13107.t32 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 4 15 -
10 g13107.t32 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 16 19 -
6 g13107.t32 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 20 210 -
4 g13107.t32 SUPERFAMILY SSF48264 Cytochrome P450 35 208 5.88E-19
3 g13107.t32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 2 19 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed