Gene loci information

Transcript annotation

  • This transcript has been annotated as 40S ribosomal protein S4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13143 g13143.t75 TSS g13143.t75 28150713 28150713
chr_1 g13143 g13143.t75 isoform g13143.t75 28150731 28152591
chr_1 g13143 g13143.t75 exon g13143.t75.exon1 28150731 28150784
chr_1 g13143 g13143.t75 exon g13143.t75.exon2 28151298 28151467
chr_1 g13143 g13143.t75 cds g13143.t75.CDS1 28151401 28151467
chr_1 g13143 g13143.t75 exon g13143.t75.exon3 28151695 28151792
chr_1 g13143 g13143.t75 cds g13143.t75.CDS2 28151695 28151792
chr_1 g13143 g13143.t75 exon g13143.t75.exon4 28152163 28152591
chr_1 g13143 g13143.t75 cds g13143.t75.CDS3 28152163 28152591
chr_1 g13143 g13143.t75 TTS g13143.t75 28152716 28152716

Sequences

>g13143.t75 Gene=g13143 Length=751
CCTAAGTAAGTGCACACAAATTTTTTAAAAATAATCAAATTGACTTGAATTATTATCAAC
CGGTCCTCATAAATTGCGTGAATCACTTCCTTTGGTCATTTTCTTGCGCAATCGCTTGAA
GTATGCTTTGACCAACACTGAAACTAAGAAAATTGTCATGCAAAGACACATCAAAGTTGA
TGGAAAAGTCAGAACAGATATCAATTATCCAGCAGGTTTCATGGATGTCATCACCATTGA
GAAAACTGGTGAATTCTTCCGTTTGATCTATGATGTAAAGGGTCGTTTTACAATCCATCG
CATTACTGCCGATGAAGCAAAATACAAGTTATGCAAAGTGAAGCGTGTAACAATTGGACC
TAAGAAGATTCCATACTTGGTCACACATGATGGTCGTACACTCCGTTATCCAGATCCAAA
CATTCATGTAAACGACACAATTCAATTAGATATTGCAACTGGAAAAATCACAGATCATAT
TCGTTTTGAATCCGGCAATTTGGTTATGGTTACTGGAGGTCGTAACTTGGGTCGAGTTGG
TACTGTTGTGAATCGTGAAAAGCATCCTGGTTCATTTGAAATCATTCATGTGAAAGACGC
TAATGGACATATGTTTGCTACTCGTTTGACAAACATTTTCATCATCGGAAAAGGCGCAAA
GGCATACATTTCATTGCCTAAAGGCAAGGGTATTAAACTCAGTGTTGCCGAAGAACGCGA
TAAGCGATTGGCACAAAAAACCACTAACTAA

>g13143.t75 Gene=g13143 Length=197
MQRHIKVDGKVRTDINYPAGFMDVITIEKTGEFFRLIYDVKGRFTIHRITADEAKYKLCK
VKRVTIGPKKIPYLVTHDGRTLRYPDPNIHVNDTIQLDIATGKITDHIRFESGNLVMVTG
GRNLGRVGTVVNREKHPGSFEIIHVKDANGHMFATRLTNIFIIGKGAKAYISLPKGKGIK
LSVAEERDKRLAQKTTN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g13143.t75 CDD cd00165 S4 1 47 0.0024118
10 g13143.t75 CDD cd06087 KOW_RPS4 110 164 0.0000000
7 g13143.t75 Gene3D G3DSA:3.10.290.40 - 1 52 0.0000000
9 g13143.t75 Gene3D G3DSA:2.40.50.740 - 57 107 0.0000000
8 g13143.t75 Gene3D G3DSA:2.30.30.30 - 110 180 0.0000000
4 g13143.t75 PANTHER PTHR11581:SF30 40S RIBOSOMAL PROTEIN S4, X ISOFORM 1 194 0.0000000
5 g13143.t75 PANTHER PTHR11581 30S/40S RIBOSOMAL PROTEIN S4 1 194 0.0000000
3 g13143.t75 Pfam PF00900 Ribosomal family S4e 30 104 0.0000000
1 g13143.t75 Pfam PF00467 KOW motif 113 145 0.0000027
2 g13143.t75 Pfam PF16121 40S ribosomal protein S4 C-terminus 147 194 0.0000000
6 g13143.t75 SMART SM00739 kow_9 109 136 0.0023000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005840 ribosome CC
GO:0003723 RNA binding MF
GO:0006412 translation BP
GO:0003735 structural constituent of ribosome MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values