General information for g1333.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein phosphatase 2B catalytic subunit 2.
Parent gene
- The parent gene g1333 is located on chr_3: 9842905..9859177 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1333 | g1333.t1 | TTS | g1333.t1 | 9842242 | 9842242 |
| chr_3 | g1333 | g1333.t1 | isoform | g1333.t1 | 9842905 | 9859177 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon1 | 9842905 | 9843086 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS1 | 9842905 | 9843086 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon2 | 9843154 | 9843251 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS2 | 9843154 | 9843251 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon3 | 9843394 | 9843478 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS3 | 9843394 | 9843478 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon4 | 9856198 | 9856405 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS4 | 9856198 | 9856405 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon5 | 9856613 | 9856706 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS5 | 9856613 | 9856706 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon6 | 9856848 | 9857211 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS6 | 9856848 | 9857211 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon7 | 9858064 | 9858175 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS7 | 9858064 | 9858175 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon8 | 9858304 | 9858410 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS8 | 9858304 | 9858410 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon9 | 9858544 | 9858765 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS9 | 9858544 | 9858765 |
| chr_3 | g1333 | g1333.t1 | exon | g1333.t1.exon10 | 9859072 | 9859177 |
| chr_3 | g1333 | g1333.t1 | cds | g1333.t1.CDS10 | 9859072 | 9859177 |
| chr_3 | g1333 | g1333.t1 | TSS | g1333.t1 | NA | NA |
Sequences
>g1333.t1 Gene=g1333 Length=1578
ATGTCGTCTGTAAAGCAAGAACAACAACAATCATTAGACCAAACACAGCAACGTGAATCC
GAAACGCAACAATCTAAGCCATCAACTACGGATAGGGTTATAGATAGCGTTAAATTTCCA
CCAAAACACAAACTAACGGTCGCTGAGGTCTTTAATGGTCGCAATGGACAGCCGAATTTA
GAACTTTTGAAAAAGTATTTTATCGCAGAAGGTCGACTGGACGAGACGACAGCAATGCGC
ATTATTCAAGATGGTGCCGCATTATTGAAAGCTGAAAATACAATGATTGATATTGAGGCT
CCTGTCACGGTGTGCGGTGACGTGCATGGTCAATTCTTCGATCTGATGAAATTGTTTGAA
GTCGGTGGCTCACCTGCGACCACCAAATATCTTTTTCTTGGCGACTATGTTGATAGAGGA
TATTTTAGCATAGAGTGCGTACTATATCTGTGGGCATTGAAGATTTGTTATCCAACAACC
TTATTTCTATTGCGAGGCAATCATGAATGTCGGCATTTGACTGAATATTTTACTTTTAAG
CAAGAATGTAAAATTAAATATTCAGAACGAGTTTACGATGCGTGCATGGAAGCGTTCGAT
TGTTTGCCATTAGCTGCATTAATGAATCAGCAATTTTTATGCGTGCACGGTGGACTGTCG
CCGGAAATTCAGGATTTGGATGATATACGAAAGCTCGATCGTTTTAAGGAACCGCCAGCA
TATGGGCCGATGTGCGATTTATTGTGGAGTGATCCATTGGAAGATTTTGGAAATGAGAAA
AATACCGACTTTTATTCACATAATACAGTTCGTGGATGTTCATACTTTTATAGCTATCGG
GCGTGTTGTGATTTCTTGCAAAATAACAATTTGTTATCCATCATTCGTGCTCACGAAGCT
CAAGATGCTGGATATCGAATGTACCGAAAGAGTCTCACAACAGGTTTTCCATCGCTTATA
ACAATTTTTTCAGCACCTAATTATCTTGATGTTTACAACAATAAGGCTGCTGTCCTTAAA
TATGAAAATAATGTAATGAATATAAGACAGTTTAACTGTTCACCACATCCCTATTGGCTT
CCAAACTTTATGGATGTTTTCACATGGTCGCTGCCATTCGTAGGTGAAAAGGTTACAGAG
ATGCTTGTTAACGTACTTAACATATGCTCGGATGATGAATTGATGAGTGAGAGTGATGAC
ACCCTAGAGGAAGGTAATGCAGCTAACCTACGCAAGGAGGTTATTAGAAATAAAATTCGT
GCAATCGGTAAAATGGCACGCGTATTTTCTGTGTTAAGAGAGGAGTCAGAATCTGTTTTG
CAATTGAAGGGGCTTACTCCTACTGGAGCGTTACCTCTTGGAGCATTATCTGGCGGCAAA
ACGTCACTTAAGAACGCATTACAAGGTTTCTCGCCGAATCATAAAATTACATCATTTGCG
GAAGCTAAGGGTCTCGATGCTATTAATGAACGCATGCCACCACGCAAAGATGCGCCACCA
ACACCATCGTCAGATGATAAACCATCGTCACATAAGGGCGATATCAAAAACGAGAAAGCA
ACCAACAATCATTCTTAA
>g1333.t1 Gene=g1333 Length=525
MSSVKQEQQQSLDQTQQRESETQQSKPSTTDRVIDSVKFPPKHKLTVAEVFNGRNGQPNL
ELLKKYFIAEGRLDETTAMRIIQDGAALLKAENTMIDIEAPVTVCGDVHGQFFDLMKLFE
VGGSPATTKYLFLGDYVDRGYFSIECVLYLWALKICYPTTLFLLRGNHECRHLTEYFTFK
QECKIKYSERVYDACMEAFDCLPLAALMNQQFLCVHGGLSPEIQDLDDIRKLDRFKEPPA
YGPMCDLLWSDPLEDFGNEKNTDFYSHNTVRGCSYFYSYRACCDFLQNNNLLSIIRAHEA
QDAGYRMYRKSLTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWL
PNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELMSESDDTLEEGNAANLRKEVIRNKIR
AIGKMARVFSVLREESESVLQLKGLTPTGALPLGALSGGKTSLKNALQGFSPNHKITSFA
EAKGLDAINERMPPRKDAPPTPSSDDKPSSHKGDIKNEKATNNHS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g1333.t1 | CDD | cd07416 | MPP_PP2B | 58 | 362 | 0.0 |
| 12 | g1333.t1 | Gene3D | G3DSA:3.60.21.10 | - | 23 | 444 | 5.8E-178 |
| 18 | g1333.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 32 | - |
| 19 | g1333.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 35 | - |
| 17 | g1333.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 485 | 525 | - |
| 16 | g1333.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 507 | 525 | - |
| 2 | g1333.t1 | PANTHER | PTHR45673:SF2 | SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT GAMMA ISOFORM | 23 | 516 | 1.4E-286 |
| 3 | g1333.t1 | PANTHER | PTHR45673 | SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED | 23 | 516 | 1.4E-286 |
| 5 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 101 | 128 | 6.0E-80 |
| 7 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 130 | 157 | 6.0E-80 |
| 10 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 163 | 187 | 6.0E-80 |
| 9 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 197 | 223 | 6.0E-80 |
| 6 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 226 | 253 | 6.0E-80 |
| 8 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 290 | 310 | 6.0E-80 |
| 4 | g1333.t1 | PRINTS | PR00114 | Serine/threonine phosphatase family signature | 318 | 334 | 6.0E-80 |
| 1 | g1333.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 101 | 301 | 2.3E-38 |
| 14 | g1333.t1 | ProSitePatterns | PS00125 | Serine/threonine specific protein phosphatases signature. | 164 | 169 | - |
| 15 | g1333.t1 | SMART | SM00156 | pp2a_7 | 73 | 364 | 4.6E-154 |
| 11 | g1333.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 31 | 493 | 4.91E-150 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0097720 | calcineurin-mediated signaling | BP |
| GO:0033192 | calmodulin-dependent protein phosphatase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04348 (serine/threonine-protein phosphatase 2B catalytic subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
