Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Vacuolar protein sorting-associated protein 4B.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13384 g13384.t4 TTS g13384.t4 29619487 29619487
chr_1 g13384 g13384.t4 isoform g13384.t4 29619496 29620442
chr_1 g13384 g13384.t4 exon g13384.t4.exon1 29619496 29620442
chr_1 g13384 g13384.t4 cds g13384.t4.CDS1 29620045 29620383
chr_1 g13384 g13384.t4 TSS g13384.t4 NA NA

Sequences

>g13384.t4 Gene=g13384 Length=947
GAAGACGTTTTGAAAAAAGAATTTATATACCTTTGCCTGAAGAAAACGCTCGCTTGGTCA
TGTTTAAAATTCATTTGGGCAACACATCGCATGTATTGACAGAAGAAAATTTAAGACAAC
TCGCAAAACAAACTGAAGGTTATTCAGGTGCTGATATTTCAATTGTAGTCCGTGATGCTC
TTATGCAACCTGTTCGAAAAGTTCAGACAGCAACACATTTTAAAAGGATTCGCGGTCCAT
CGCCTTTGGATAAAAATGTAATGGTAGATGATCTTCTTACACCTTGCTCGCCTGGTGAAA
AAGGAGCAATTGAAATGACTTGGATGGAAGTTGAGGGAGAGAAACTTTTTGAACCTCCAG
TAACAATGGTAATGGTATCATTTTATTTAAGTATTTAAGAAATAAAAAAGTATTTTTTTT
TTCATAGGGTGACATGCTTAATTCACTTGCAAGAACAAAACCAACAATCAATGAAGACGA
TATGAAGAAACTGCAAAAGTTCACTGACGATTTTGGTCAGGAGGGTTAAAAAATTGTACG
GAATTCAACTATTTTCTATTTAAATATTATATATTGCGAGAGTAAAATGTGCATGAGAAG
AAGAACGTTGCCTATATTTAAACATACATACTTTTAGATATGATGAAAAGTTATTTCATT
CACAATAAGCGATGATGATGATGAAGTTGATTTTACGCATACATGTCTCGTGATGAAATA
TGCTATTAATATTAATATAACCCAAGAGAGAAATACAAAGTTTTATTATACAGAAAAACC
GGATTTTTCATACCAACTCTGAAACAATATGGAATTTTTTACTTAACAGATTCAAATCAC
ACAGAAAAATTAAGTAATCAAATGACTAATTTTTAATATCTTTTCTTCTCTGTTTGTTCT
TAAAAAATAATTGATGTTGCGTTTAAAAAGAAATAAAGTAAAAAAAT

>g13384.t4 Gene=g13384 Length=112
MFKIHLGNTSHVLTEENLRQLAKQTEGYSGADISIVVRDALMQPVRKVQTATHFKRIRGP
SPLDKNVMVDDLLTPCSPGEKGAIEMTWMEVEGEKLFEPPVTMVMVSFYLSI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g13384.t4 Gene3D G3DSA:1.10.8.60 - 1 111 0
3 g13384.t4 PANTHER PTHR23074:SF72 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4B 1 104 0
4 g13384.t4 PANTHER PTHR23074 AAA DOMAIN-CONTAINING 1 104 0
2 g13384.t4 Pfam PF17862 AAA+ lid domain 17 50 0
1 g13384.t4 Pfam PF09336 Vps4 C terminal oligomerisation domain 73 103 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values