Gene loci information

Transcript annotation

  • This transcript has been annotated as Vacuolar protein sorting-associated protein 4B.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13384 g13384.t8 TTS g13384.t8 29619487 29619487
chr_1 g13384 g13384.t8 isoform g13384.t8 29619496 29621850
chr_1 g13384 g13384.t8 exon g13384.t8.exon1 29619496 29620015
chr_1 g13384 g13384.t8 cds g13384.t8.CDS1 29619914 29620015
chr_1 g13384 g13384.t8 exon g13384.t8.exon2 29620075 29620778
chr_1 g13384 g13384.t8 cds g13384.t8.CDS2 29620075 29620383
chr_1 g13384 g13384.t8 exon g13384.t8.exon3 29620852 29621086
chr_1 g13384 g13384.t8 exon g13384.t8.exon4 29621146 29621284
chr_1 g13384 g13384.t8 exon g13384.t8.exon5 29621346 29621509
chr_1 g13384 g13384.t8 exon g13384.t8.exon6 29621708 29621850
chr_1 g13384 g13384.t8 TSS g13384.t8 29621850 29621850

Sequences

>g13384.t8 Gene=g13384 Length=1905
ACAACAACAACATTGAAAATAATAAAATTGTGCAAAAAATTTATAAATTTGAGAAAAATA
GCATTAAAATCAAATATCGACACTTTATTATCCTTCATTTGTTAAAATAACAACAATTGC
TGCTTAAGTGACAGTAAAACAAGAAGTTGGATCAAAAATCAATAACTTATAATGGCTGCT
GGAACAACTTTACAAAAAGCTATTGATATAGTAACGAAAGCAACAGAGGAAGATAGAAAT
AAAAATTATGAGGAAGCATTAAGGCTATATGAGCATGGTGTTGAATATTTTCTTCACGCT
ATTAAATACGAAGCTCAAGGTGACAAAGCAAAAGAATCGATTAGAGCAAAATGTTTACAG
TATCTTGATCGTGCAGAGAAACTCAAACAATATTTGAAAAAAGGAAAAAAGCAGAAACCT
GTAAAAGATGGAGATACAGGTTCCAAAGATAAAGATAAGAAAAGTGATAGTGATTCAGAT
GAAGATGATCCAGAGAAAAAGAAACTTCAGAGTAAATTGGAAGGCGCAATTGTTGTTGAG
AAACCTAAGGTTAAATGGTCAGATGTAGCCGGTCTCGAAGGCGCTAAGGAAGCGCTTAAA
GAAGCAGTCATTCTGCCAATAAAATTTCCTCATTTATTTACTGGAAAAAGAGTTCCATGG
AAAGGAATTTTACTCTTTGGGGTACTGGTAAATCTTATTTGGCTAAAGCTGTTGCAACAG
AAGCAAATAATTCTACATTCTTCTCTGTATCGAGTGCTGATTTGGTTTCAAAATGGCTCG
GTGAATCAGAAAAGCTCGTTAGAAATCTCTTTGACTTGGCTCGTGCTCACAAGCCTAGTA
TCATCTTTATCGATGAAGTTGACTCTTTATGTTCTTCTCGTTCTGAAAACGAAAGTGAAA
GTGCCCGTCGTATCAAAACCGAATTTCTTGTTCAAATGCAAGGTGTAGGCAATGATAATG
AAGGAATTCTCGTTCTTGGTGCGACAAATATTCCATGGGTTCTTGATTCTGCTATTAGAA
GACGTTTTGAAAAAAGAATTTATATACCTTTGCCTGAAGAAAACGCTCGCTTGGTCATGT
TTAAAATTCATTTGGGCAACACATCGCATGTATTGACAGAAGAAAATTTAAGACAACTCG
CAAAACAAACTGAAGGTTATTCAGGTGCTGATATTTCAATTGTAGTCCGTGATGCTCTTA
TGCAACCTGTTCGAAAAGTTCAGACAGCAACACATTTTAAAAGGATTCGCGGTCCATCGC
CTTTGGATAAAAATGTAATGGTAGATGATCTTCTTACACCTTGCTCGCCTGGTGAAAAAG
GAGCAATTGAAATGACTTGGATGGAAGTTGAGGGAGAGAAACTTTTTGAACCTCCAGTAA
CAATGGGTGACATGCTTAATTCACTTGCAAGAACAAAACCAACAATCAATGAAGACGATA
TGAAGAAACTGCAAAAGTTCACTGACGATTTTGGTCAGGAGGGTTAAAAAATTGTACGGA
ATTCAACTATTTTCTATTTAAATATTATATATTGCGAGAGTAAAATGTGCATGAGAAGAA
GAACGTTGCCTATATTTAAACATACATACTTTTAGATATGATGAAAAGTTATTTCATTCA
CAATAAGCGATGATGATGATGAAGTTGATTTTACGCATACATGTCTCGTGATGAAATATG
CTATTAATATTAATATAACCCAAGAGAGAAATACAAAGTTTTATTATACAGAAAAACCGG
ATTTTTCATACCAACTCTGAAACAATATGGAATTTTTTACTTAACAGATTCAAATCACAC
AGAAAAATTAAGTAATCAAATGACTAATTTTTAATATCTTTTCTTCTCTGTTTGTTCTTA
AAAAATAATTGATGTTGCGTTTAAAAAGAAATAAAGTAAAAAAAT

>g13384.t8 Gene=g13384 Length=136
MFKIHLGNTSHVLTEENLRQLAKQTEGYSGADISIVVRDALMQPVRKVQTATHFKRIRGP
SPLDKNVMVDDLLTPCSPGEKGAIEMTWMEVEGEKLFEPPVTMGDMLNSLARTKPTINED
DMKKLQKFTDDFGQEG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g13384.t8 Gene3D G3DSA:1.10.8.60 - 1 115 0
5 g13384.t8 Gene3D G3DSA:3.40.50.300 - 116 125 0
3 g13384.t8 PANTHER PTHR23074:SF72 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4B 1 134 0
4 g13384.t8 PANTHER PTHR23074 AAA DOMAIN-CONTAINING 1 134 0
2 g13384.t8 Pfam PF17862 AAA+ lid domain 17 50 0
1 g13384.t8 Pfam PF09336 Vps4 C terminal oligomerisation domain 73 133 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values