General information for g13390.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 307a1.
Parent gene
- The parent gene g13390 is located on chr_1: 29663579..29665378 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13390 | g13390.t1 | isoform | g13390.t1 | 29663579 | 29665378 |
| chr_1 | g13390 | g13390.t1 | exon | g13390.t1.exon1 | 29663579 | 29663910 |
| chr_1 | g13390 | g13390.t1 | cds | g13390.t1.CDS1 | 29663579 | 29663910 |
| chr_1 | g13390 | g13390.t1 | exon | g13390.t1.exon2 | 29663992 | 29664262 |
| chr_1 | g13390 | g13390.t1 | cds | g13390.t1.CDS2 | 29663992 | 29664262 |
| chr_1 | g13390 | g13390.t1 | exon | g13390.t1.exon3 | 29664328 | 29664860 |
| chr_1 | g13390 | g13390.t1 | cds | g13390.t1.CDS3 | 29664328 | 29664860 |
| chr_1 | g13390 | g13390.t1 | exon | g13390.t1.exon4 | 29664928 | 29665378 |
| chr_1 | g13390 | g13390.t1 | cds | g13390.t1.CDS4 | 29664928 | 29665378 |
| chr_1 | g13390 | g13390.t1 | TSS | g13390.t1 | 29665657 | 29665657 |
| chr_1 | g13390 | g13390.t1 | TTS | g13390.t1 | NA | NA |
Sequences
>g13390.t1 Gene=g13390 Length=1587
ATGTACAACTCAATGCTTTTGCTGTTCAGTTCGAACACAATTTATATTATGATAACGTGC
TTCATTCTTACATTGATTCTTCTCGCAAATGAGCTTAATTTTACGCAAATTAAAAAGAAA
ATGCTCTTTAGAGGTCGCCATATGATGGAAATTCAATTAGGCCATAATGAAAAGAAAAAT
TTCGGCGCAAAACAGGCAACAGGACCAAAACGTAAATTTATTATTGGCAATCTTGATGTG
CTCGATGGCTACGAAATTCCATATATGGCATTCAGTGACTTGGCCCAAAAATATGGCAAT
ATTTTCAAGTTACAAATGGGAACTGTACCAAGTATTGTTGTCAATGGTTTGGAAAATATT
CGTGAAGTCCTCATTCATAAGGGTGACCATTTTGACTCACGTCCTGGCTTCAAACGTTAC
CATATGTTGTTCGATGGAAATAAGGAAAATTCACTCGCATTTTGCGATTGGTCAGATGTT
CAAAAAACCAGACGTGACATGCTCTCAACACACGCATTTCCACGCAAATTCTCGATGAGT
TTCAACCGTTTTAATGACATTACACTCGAACACTATCAGATGCTTTCCGACGATATTCGA
CGAGACATCATAAGTGGTAAGACGACTGCAATTAAGCCACTTATAGTTGAAGCATGTGGA
AATGTTTTCACTCAATACTTCACCACACGTTCATTTGAACGAGGCAACACCAAGTTCCAG
CAATTACTAAAGAATTTCGATAAAATTTTCTGGGAAGTAAACCAAGGCTATGCTGCTGAT
TTTCTGCCTTTCCTCTTGCCATTCCATAGCAAGCGTATGAAGAAATTGGAACAGTGGTCA
CACGAAATTCGTCACTTTATTGTCGAGAATATCATTGGTGATAGATATGAAACATGGACT
GTTAGCAACGAACCAAATGATTACATTGAATCACTTATTGACCACGTGAAACAAAAATTA
CAGCCAACTATTGAATGGGATACGGCTTTATTTGCATTGGAAGATATTATCGGTGGACAT
GCGGCTGTAGCAAATTTCTTAGTCAAAGTTATCGCTTTTATTGCACAAAATAAGGAAGTT
CAGAAAAATATTCAAGCGGAAGTTGACAATTTGCTAAATGAACGCAGTGAAAAGTCAGTG
CTGATAGGAGATCGCAATAAATTAATTTACACTGAAGCAACAGTAATGGAAGCATTGAGA
TTAGTCTCATCACCGATTGTACCGCATGTTGCGTCACAAGACAGCACCATTGATGGTTTT
GAAGTAAAAACCGGCACATTAATTTTCATTAACAATTACGATTTAAATATGTCAACAAAA
CTCTGGGAGGCACCTGAAAAATTTGAACCAAAAAGATTCATTTCAAACGGTCGACTACTG
AAGCCCGATCATTTTATTCCTTTTGGCAATGGAAAACGTTCATGTATGGGCTATAAACTC
GTTCAATTCTTATCATTCGCTATACTTGGAAATTTAATGAAGGACTTTGACATCAGTACA
GAAAATGGTGAGGAAATTAAAGTACCACTCGGTTCACTTGCAGTGACAGAAAATCCTTAT
CGATTGGCATTTACTCTAAGAAATTAA
>g13390.t1 Gene=g13390 Length=528
MYNSMLLLFSSNTIYIMITCFILTLILLANELNFTQIKKKMLFRGRHMMEIQLGHNEKKN
FGAKQATGPKRKFIIGNLDVLDGYEIPYMAFSDLAQKYGNIFKLQMGTVPSIVVNGLENI
REVLIHKGDHFDSRPGFKRYHMLFDGNKENSLAFCDWSDVQKTRRDMLSTHAFPRKFSMS
FNRFNDITLEHYQMLSDDIRRDIISGKTTAIKPLIVEACGNVFTQYFTTRSFERGNTKFQ
QLLKNFDKIFWEVNQGYAADFLPFLLPFHSKRMKKLEQWSHEIRHFIVENIIGDRYETWT
VSNEPNDYIESLIDHVKQKLQPTIEWDTALFALEDIIGGHAAVANFLVKVIAFIAQNKEV
QKNIQAEVDNLLNERSEKSVLIGDRNKLIYTEATVMEALRLVSSPIVPHVASQDSTIDGF
EVKTGTLIFINNYDLNMSTKLWEAPEKFEPKRFISNGRLLKPDHFIPFGNGKRSCMGYKL
VQFLSFAILGNLMKDFDISTENGEEIKVPLGSLAVTENPYRLAFTLRN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g13390.t1 | Coils | Coil | Coil | 354 | 374 | - |
| 11 | g13390.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 57 | 528 | 3.7E-89 |
| 2 | g13390.t1 | PANTHER | PTHR24303:SF27 | CYTOCHROME P450 307B1 | 20 | 527 | 5.2E-203 |
| 3 | g13390.t1 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 20 | 527 | 5.2E-203 |
| 6 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 95 | 114 | 3.7E-29 |
| 8 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 119 | 140 | 3.7E-29 |
| 9 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 347 | 373 | 3.7E-29 |
| 5 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 431 | 455 | 3.7E-29 |
| 7 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 465 | 475 | 3.7E-29 |
| 4 | g13390.t1 | PRINTS | PR00463 | E-class P450 group I signature | 475 | 498 | 3.7E-29 |
| 1 | g13390.t1 | Pfam | PF00067 | Cytochrome P450 | 68 | 522 | 3.1E-65 |
| 14 | g13390.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 11 | - |
| 15 | g13390.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 34 | - |
| 13 | g13390.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 35 | 528 | - |
| 17 | g13390.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 468 | 477 | - |
| 10 | g13390.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 68 | 527 | 4.45E-77 |
| 16 | g13390.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K14939 (cytochrome P450 family 307 subfamily A)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed